The levels of high mobility group box 1 (HMGB1) and myeloperoxidase (MPO) in the plasma were measured by ELISA according to the manufacturer’s instructions by using HMGB1 kit (IBL International GmbH, Hamburg, Germany) and MPO kit (Hycult Biotech, Uden, Netherlands), respectively. The respective levels of proinflammatory cytokine (interleukin 6 (IL-6), IL-8, tumor necrosis factor α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory proteins-1β (MIP-1β), and granulocyte-colony stimulating factor (G-CSF)) and anti-inflammatory cytokine (IL-5, L-10, and IL-13) present in the plasma samples were analyzed using a Luminex assay kit (Bio-Rad Laboratories, Hercules, CA).
Mpo kit
Manufactured by Hycult Biotech
Sourced in Germany
The MPO kit is a laboratory equipment product designed for the quantitative measurement of myeloperoxidase (MPO) levels. MPO is an enzyme found in the azurophilic granules of neutrophils and plays a role in the immune response. The kit provides the necessary reagents and protocols to perform this measurement in a research setting.
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Lab products found in correlation
2 protocols using mpo kit
1
Plasma Biomarkers of Inflammation
2
Quantification of Neutrophil Extracellular Traps
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The myeloperoxidase–DNA (MPO–DNA) complexes were quantified using the Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Invitrogen, USA) according to the manufacturer's instructions [17 (link)]. To quantify NETs in the cell culture supernatant and plasma, a capture ELISA of myeloperoxidase (MPO) associated with DNA was performed. For the capture antibody, an MPO kit (Hycult Biotech, HK210-01) was used.
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