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3 protocols using cyfluthrin

1

Insecticides and Synergist Evaluation

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A total of 20 insecticides (representing diverse structures and known stereochemical composition) and three synergists were used (Fig 3): 1R-cis αS cypermethrin (100%, Bayer CropScience, Leverkusen, Germany), 1R-trans fenfluthrin (100%, Bayer CropScience), alpha-cypermethrin (99.5%, Chem Service, West Chester, PA, USA), bioallethrin (3.8% cis and 95.7% trans, Chem Service), bioresmethrin (99.5%, Chem Service), cis-permethrin (99.4%, Zeneca Ag products), cyfluthrin (99.2%, Chem Service), cyhalothrin (90.2%, ICI Americas), cypermethrin (44.8% cis and 53.9% trans, Chem Service), N-decarbomethoxylated JW062 (DCJW) (98%, DuPont), DDT (98%, Sigma-Aldrich, St. Louis, MO, USA), deltamethrin (99.5%, Chem Service), diethyl maleate (DEM) (97%, Sigma-Aldrich), etofenprox (96.3%, Mitsui Toatsu Chemicals, Tokyo Japan), flumethrin (100%, Bayer CropScience), permethrin (24% cis and 76% trans, Chem Service), piperonyl butoxide (PBO) (90%, Sigma-Aldrich), S,S,S-tributyl phosphorotrithioate (DEF) (97.3%, Chem Service), tau-fluvalinate (96%, Chem Service), tefluthrin (96%, Sigma-Aldrich), transfluthrin (99.5%, Chem Service), trans-permethrin (99%, FMC, Philadelphia, PA, USA).
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2

Synthesis and Characterization of Pyrethroids

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Bifenthrin (part #: N-11203; CAS: 82657-04-3), cyfluthrin (part #: N-11130; CAS: 68359-37-5), cyhalothrin (part #: N-12307; CAS: 91465-08-6), cypermethrin (part #: N-11545; CAS: 52315-07-8), esfenvalerate (part #: N-11102; CAS: 66230-04-4), and permethrin (part #: N-12848; CAS: 52645-53-1) were purchased from Chem Service (West Chester, PA, USA). HPLC-grade methanol used to make stock solutions was purchased from Fisher Scientific (Waltham, MA, USA).
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3

Analytical Standards for Insecticides

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Analytical standards of Bifenthrin, Tefluthrin, cyfluthrin, and tebupirimphos were used, which were the active ingredients in the formulated soil insecticides tested in the field. Bifenthrin 98%, cyfluthrin 99.2%, and tebupirimphos 97% were obtained from Chem Service Inc. (West Chester, PA, US; Cat. Nos. N-11203/CAS 82657-04-3, N-11130/ CAS 68359-37-5, and N-13503/CAS 96182-53-5, respectively). Tefluthrin ≥95% was provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, US; Cat. No. sc-236965/CAS 79538-32-2). All insecticides were dissolved in acetone ≥99.9% supplied by Sigma-Aldrich Corp. (St. Louis, MO, US; Cat. No. 650501/CAS 67-64-1) for stock solution preparation.
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