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Cyp2b6

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CYP2B6 is a cytochrome P450 enzyme that plays a role in the metabolism of various drugs and xenobiotics. It is responsible for the oxidative metabolism of substrates such as certain medications, environmental chemicals, and endogenous compounds. The core function of CYP2B6 is to catalyze the biotransformation of these substrates, which is an important step in their elimination from the body.

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Lab products found in correlation

Methoxyresorufin, by Merck Group (1 mentions) Pentoxyresorufin, by Merck Group (1 mentions) Ethoxyresorufin, by Merck Group (1 mentions) Benzyloxyresorufin, by Merck Group (1 mentions) Cyp2e1, by Santa Cruz Biotechnology (1 mentions) 1 chloro 2 4 dinitrobenzene, by Merck Group (1 mentions) O phenylenediamine dihydrochloride, by Merck Group (1 mentions) Microtiter plates maxisorp, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) C ebpα, by Santa Cruz Biotechnology (1 mentions) Ab36593, by Abcam (1 mentions) Ppp2r5a, by Abcam (1 mentions) Hdac1 05 100 1, by Merck Group (1 mentions) Gradient gel, by Bio-Rad (1 mentions) Parp 1, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using cyp2b6

1

Cytochrome P450 Activity Assays

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Bovine serum albumin (BSA), NADPH, 1-chloro-2,4-dinitrobenzene (CNDB), glutathione, o-phenylenediamine dihydrochloride (OPD), ethoxyresorufin, methoxyresorufin, benzyloxyresorufin and pentoxyresorufin, were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). Microtiter plates (MaxiSorp) were purchased from Nunc (Roskilde, Denmark). Polyclonal CYP1A1 antibody was purchased from Biosense Laboratories, (Bergen, Norway). CYP2B6, CYP2E1 and CYP2B1/2 antibodies were purchased from Santa Cruz Biotechnology (Dallas TX, USA). The CYP3A antiserum was a kind gift from Dr. Malin Celander. All other chemicals were of the highest commercially available grade.
Arukwe A., Røsbak R., Adeogun A.O., Langberg H.A., Venter A., Myburgh J., Botha C., Benedetti M, & Regoli F. (2015). Biotransformation and Oxidative Stress Responses in Captive Nile Crocodile (Crocodylus niloticus) Exposed to Organic Contaminants from the Natural Environment in South Africa. PLoS ONE, 10(6), e0130002.
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2

Protein Extraction and Immunoblotting Protocol

Cited 2 times
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Protein extracts were prepared as previously described.5 (link),6 (link) Nuclear extracts (50 μg) were loaded on 4%–20% gradient gels (Bio-Rad, Hercules, CA) and transferred to nitrocellulose membranes (Bio-Rad). For the investigations of PP2Ac, cytoplasmic nuclear and whole-cell extracts were used. Because C/EBPα is detected mainly in nuclei, Co-IP was performed with nuclear extracts using an improved Trueblot (Rockland Immunochemical, Limerick, PA) protocol as described.5 (link) Antibodies used for Western blot and Co-IP were as follows: PP2Ac (clone7A6; Millipore Sigma, Burlington, MA), PPP2R5A (ab89621; Abcam, Cambridge, MA), PARP1 (Santa Cruz), cyclin D1 (sc-753; Santa Cruz), Gank (12985S; Cell Signaling Technologies, Danvers, MA), cdc2 (sc-954; Santa Cruz), CY3PA4 (sc-30621; Santa Cruz), CYP2B6 (sc-55924; Santa Cruz), CYP7A1 (E-10; Santa Cruz), PEPCK (F-3; Santa Cruz), HDAC1 (05-100-I; EMD Millipore), C/EBPα (sc-61; Santa Cruz), p21 (sc-6246; Santa Cruz), HNF4α (PP-K9218-00; Perseus Proteomics), Sp5 (ab36593; Abcam), and β-actin (A5316; Sigma). Antibodies to ph-S190-C/EBPα have been described in our previous article.5 (link) Western blot and Co-IP experiments were performed 3 times which each biological replicate.
Rivas M., Johnston ME I.I., Gulati R., Kumbaji M., Margues Aguiar T.F., Timchenko L., Krepischi A., Shin S., Bondoc A., Tiao G., Geller J, & Timchenko N. (2021). HDAC1-Dependent Repression of Markers of Hepatocytes and P21 Is Involved in Development of Pediatric Liver Cancer. Cellular and Molecular Gastroenterology and Hepatology, 12(5), 1669-1682.
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