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C18 spherisorb s5 ods2

Manufactured by Waters Corporation
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The C18 Spherisorb S5 ODS2 is a reversed-phase high-performance liquid chromatography (HPLC) column. It features silica-based packing material with chemically bonded octadecyl (C18) functional groups. This column is designed for the separation and analysis of a wide range of compounds, including polar and non-polar molecules.

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Lab products found in correlation

P4000, by Thermo Fisher Scientific (3 mentions) As3000 autosampler, by Thermo Fisher Scientific (3 mentions) Fl3000 fluorescence detector, by Thermo Fisher Scientific (3 mentions) Scm1000, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

Spherisorb S5 ODS2 Spherisorb ODS2 (C18; Spherisorb ODS2 Spherisorb S5 Spherisorb C18

3 protocols using c18 spherisorb s5 ods2

1

HPLC-DAD-FD Analysis of Polyphenols

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Condensed water extracts were analyzed using HPLC-DAD-FD system. An HPLC gradient pump (P4000, ThermoFinnigan) was coupled with a vacuum membrane degasser (SCM1000, ThermoFinnigan), an AS3000 autosampler (ThermoFinnigan), a UV6000 diode array detector and a FL3000 fluorescence detector (ThermoFinnigan). Separations of polyphenols were carried out using a reversed-phase HPLC column C18 Spherisorb S5 ODS2 (Waters, 250 mm x 4 mm, 5 µm). Column temperature was set at 40 °C and injection volume was 20 μL.
Mobile phases for the determination of polyphenols in standard solutions and condensate water samples was 5% methanol-0.1% formic acid in water (eluent A) and 95% methanol-0.1% formic acid in water (eluent B). The gradient was as follows: 0-5 min, 100% A; 5-45 min, linear gradient up to 100% B; 45-55 min 100% B; 55-57 min, linear gradient up to 100% A. Post-run time was 15 min. Elution was performed at a solvent flow rate of 0.8 mL min-1. Table S1 summarized the retention time (tR), solvent used for stock solution preparation and spectra information (UV absorbance (λmax) and fluorescence (λexcitation/ λemission)) of 31 polyphenols standards separated using the above conditions. ChromQuest™ 4.2 Chromatography Data System was used to carry out HPLC-DAD/FD control, data acquisition and data analysis.
Gonzalez-Rivera J., Duce C., Campanella B., Bernazzani L., Ferrari C., Tanzini E., Onor M., Longo I., Ruiz J.C., Tinè M.R, & Bramanti E. (2021). In situ microwave assisted extraction of clove buds to isolate essential oil, polyphenols, and lignocellulosic compounds. Industrial Crops & Products., 161.
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2

HPLC Quantification of Antioxidant Compounds

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RA in the extracts was quantified using an HPLC with a diode array detector. An HPLC gradient pump (P4000, Thermo Finnigan, Thermo Finnigan Italia S.p.A., Milan, Italy) was coupled with a vacuum membrane degasser (SCM1000, ThermoFinnigan), an AS3000 autosampler (Thermo Finnigan), a UV6000 diode array detector, and a FL3000 fluorescence detector (Thermo Finnigan). Separations were carried out using a reversed-phase HPLC column C18 Spherisorb S5 ODS2 (Waters, 250 mm × 4 mm, 5 µm). The column temperature was set at 40 °C and the injection volume was 2 μL. Mobile phases were 5% methanol–95% of 5 mM sulphuric acid in water (eluent A) and 95% methanol–5% of 5 mM sulphuric acid in water (eluent B). The gradient was as follows: 0–5 min, 80% A; 5–20 min, linear gradient up to 80% B; 20–22 min, linear gradient up to 100% B; 22–45 min 100% B. Post-run time was 15 min. Elution was performed at a solvent flow rate of 0.8 mL min−1. Detection was performed in absorbance at 220, 280, and 350 nm and in fluorescence setting the excitation and emission wavelengths at 280 and 365 nm, respectively. The ChromQuest™ Thermo Finnigan 4.2 Chromatography Data System was used to carry out HPLC-DAD/FD control, data acquisition, and data analysis.
Campanella B., Simoncini M., Passaglia E., Cicogna F., Ciancaleoni G., González-Rivera J., Bernazzani L, & Bramanti E. (2024). Ecofriendly Preparation of Rosmarinic Acid-poly(vinyl alcohol) Biofilms Using NADES/DES, Ultrasounds and Optimization via a Mixture-Process Design Strategy. Materials, 17(2), 377.
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3

HPLC-DAD-FD Analysis of Polyphenols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Condensed water extracts were analyzed using HPLC-DAD-FD system. An HPLC gradient pump (P4000, ThermoFinnigan) was coupled with a vacuum membrane degasser (SCM1000, ThermoFinnigan), an AS3000 autosampler (ThermoFinnigan), a UV6000 diode array detector and a FL3000 fluorescence detector (ThermoFinnigan). Separations of polyphenols were carried out using a reversed-phase HPLC column C18 Spherisorb S5 ODS2 (Waters, 250 mm x 4 mm, 5 µm). Column temperature was set at 40 °C and injection volume was 20 μL.
Mobile phases for the determination of polyphenols in standard solutions and condensate water samples was 5% methanol-0.1% formic acid in water (eluent A) and 95% methanol-0.1% formic acid in water (eluent B). The gradient was as follows: 0-5 min, 100% A; 5-45 min, linear gradient up to 100% B; 45-55 min 100% B; 55-57 min, linear gradient up to 100% A. Post-run time was 15 min. Elution was performed at a solvent flow rate of 0.8 mL min-1. Table S1 summarized the retention time (tR), solvent used for stock solution preparation and spectra information (UV absorbance (λmax) and fluorescence (λexcitation/ λemission)) of 31 polyphenols standards separated using the above conditions. ChromQuest™ 4.2 Chromatography Data System was used to carry out HPLC-DAD/FD control, data acquisition and data analysis.
González‐Rivera J., Duce C., Campanella B., Bernazzani L., Ferrari C., Tanzini E., Onor M., Longo I., Cabrera-Ruiz J., Tiné M.R., & Bramanti E. (2021). In situ microwave assisted extraction of clove buds to isolate essential oil, polyphenols, and lignocellulosic compounds. Industrial Crops and Products, 161, 113203-113203.
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