Powerix70

Manufactured by Olympus

The PowerIX70 is a versatile high-performance centrifuge designed for a wide range of laboratory applications. It features a maximum speed of 17,000 rpm and a maximum relative centrifugal force of 30,130 xg. The centrifuge offers a comprehensive selection of rotors, accommodating sample volumes from 0.2 mL to 1 liter. The PowerIX70 is equipped with a user-friendly control panel and advanced safety features to ensure reliable and efficient operation.

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Lab products found in correlation

A10042, by Thermo Fisher Scientific (3 mentions) Ab150107, by Abcam (2 mentions) Ab121893, by Abcam (2 mentions) Ab150129, by Abcam (2 mentions) Ab135813, by Abcam (2 mentions) Ls b2138, by LifeSpan BioSciences (1 mentions) A 21424, by Thermo Fisher Scientific (1 mentions) Ab24590, by Abcam (1 mentions)

3 protocols using powerix70

Immunofluorescence Staining of Endothelial Markers

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Samples were stained following the protocol previously described [14] . The antibodies used were: CD144 (rabbit, LS-B2138, LifeSpan Biosciences), Stabilin-2 (rabbit, ab121893, abcam), Stabilin-1 (mouse, H00023166-M05, Abnova), CD31-PE (mouse, 555446,BD Bioscience), anti-rabbit Alexa Fluor 568 (donkey, A10042, Thermofisher), anti-mouse Alexa Fluor 647 (donkey, ab150107, abcam) and antimouse (goat, A21424, Life Technologies). All fluorescent images were taken using a confocal microscope (PowerIX70, Olympus).

Danoy M., Poulain S., Koui Y., Tauran Y., Scheidecker B., Kido T., Miyajima A., Sakai Y., Plessy C, & Leclerc E. (2020). Transcriptome profiling of hiPSC-derived LSECs with nanoCAGE. Molecular omics, 16(2).

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Multicolor Immunofluorescence Staining

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Staining was performed following the protocol detailed in supplementary material File 1. The antibodies used in this study were goat anti-albumin (A80–129A, Bethyl), rabbit anti-CYP3A4 (ab135813, abcam), rabbit anti-Stabilin-2 (ab121893, abcam), mouse anti-PECAM-1 (ab24590, abcam), donkey anti-goat Alexa Fluor 488 (ab150129, abcam), donkey anti-rabbit Alexa Fluor 568 (A10042, Thermofisher), and donkey anti-mouse Alexa Fluor 647 (ab150107, abcam). Fluorescent images were captured using a confocal microscope (PowerIX70, Olympus).

Danoy M., Tauran Y., Poulain S., Jellali R., Bruce J., Leduc M., Le Gall M., Koui Y., Arakawa H., Gilard F., Gakiere B., Kato Y., Plessy C., Kido T., Miyajima A., Sakai Y, & Leclerc E. (2021). Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment. APL Bioengineering, 5(2), 026104.

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Immunostaining of Liver Cell Markers

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Cells were fixed in 4% paraformaldehyde overnight at 4°C, washed and stored in PBS. Immunostaining was performed following the protocol previously described (Danoy et al., 2019) . The antibodies used were: Albumin (goat, A80-129A, Bethyl), CYP3A4 (rabbit, ab135813, abcam), anti-goat Alexa Fluor 488 (donkey, ab150129, abcam), and anti-rabbit Alexa Fluor 568 (donkey, A10042, Thermofisher). All fluorescent images were taken using a confocal microscope (PowerIX70, Olympus).

Danoy M., Poulain S., Lereau-Bernier M., Kato S., Scheidecker B., Kido T., Miyajima A., Sakai Y., Plessy C, & Leclerc E. (2020). Characterization of liver zonation-like transcriptomic patterns in HLCs derived from hiPSCs in a microfluidic biochip environment. Biotechnology progress, 36(5).

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