Immpress hrp anti mouse igg peroxidase polymer detection kit

Manufactured by Vector Laboratories

The ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit is a ready-to-use, enzyme-labeled secondary antibody system designed for the detection of mouse primary antibodies in immunohistochemical and other immunoassay procedures. The kit contains a peroxidase-labeled polymer conjugated to anti-mouse IgG antibodies.

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Lab products found in correlation

Axioplan 2 microscope, by Zeiss (1 mentions) Immpact dab peroxidase substrate, by Vector Laboratories (1 mentions) Evos m7000, by Thermo Fisher Scientific (1 mentions) Immpact dab peroxidase hrp substrate, by Vector Laboratories (1 mentions) Vectamount permanent mounting medium, by Vector Laboratories (1 mentions) Ab68428, by Abcam (1 mentions) Anti actin, by Merck Group (1 mentions) Vector blue alkaline phosphatase blue ap substrate kit, by Vector Laboratories (1 mentions) Mab377, by Abcam (1 mentions) Ab955, by Abcam (1 mentions)

Close spelling variants of this product

ImmPRESS detection kit (8 citations) ImmPRESS™ anti-mouse IgG (7 citations) ImmPRESS kits (3 citations) ImmPRESS-HRP detection kit (2 citations)

4 protocols using immpress hrp anti mouse igg peroxidase polymer detection kit

Immunohistochemical Evaluation of p62 in HNSCC

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HNSCC blocks were retrieved from the Pathology core at Wayne State University and sections were deparaffinized and then hydrated. Microwave heat-induced antigen retrieval was performed using the Antigen Citrus Plus Retrieval Solution (BioGenex, HK081-5K). Non-specific binding sites were blocked with 2.5% normal horse serum in a wet chamber at 4 ^oC, overnight. p62 (AbCam, ab56416) was diluted 1:2000 in blocking solution then incubated in a wet chamber at 4 ^oC for 6 h. The ImmPRESS™ HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories, MP-7402) and the ImmPACT^TM DAB Peroxidase Substrate (Vector Laboratories, SK-4105) were used to develop the signal of stained slides followed by counterstaining with Mayer’s Hematoxylin (Scytek, HMM999). Images were obtained using a Zeiss Axioplan 2 microscope. Scoring was performed by a pathologist using a scale of 0 = no staining, 1 = low, 2 = moderate, 3 = high stain.

Lee S.H., Cho W.J., Najy A.J., Saliganan A.D., Pham T., Rakowski J., Loughery B., Ji C.H., Sakr W., Kim S., Kato I., Chung W.K., Kim H.E., Kwon Y.T, & Kim H.R. (2021). p62/SQSTM1-induced caspase-8 aggresomes are essential for ionizing radiation-mediated apoptosis. Cell Death & Disease, 12(11), 997.

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Immunohistochemical Analysis of Tissue Samples

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For IHC, 4 μm thick sections were cut from paraffin-embedded blocks and dried onto positively charged microscope slides. Slides were prepared and stained using the ImmPRESS® HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories) as previously described [23 (link)].
For analysis, tissue sections were imaged using an EVOS M7000 automated microscope (Thermo Fisher Scientific). Images were de-identified and 50 random fields per section were run through QuPath image analysis software [24 (link)] to quantify positive DAB-stained cells (as a percentage of total cells).

Morel K.L., Hamid A.A., Clohessy J.G., Pandell N., Ellis L, & Sweeney C.J. (2021). NF-κB blockade with oral administration of dimethylaminoparthenolide (DMAPT), delays prostate cancer resistance to androgen receptor (AR) inhibition and inhibits AR variants. Molecular cancer research : MCR, 19(7), 1137-1145.

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IHC Analysis of CRC Tissue Sections

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IHC analysis was performed for CRC tissue sections as described earlier [20] (link), [21] , [22] (link). Briefly, sections were deparaffinized, rehydrated, antigen-retrieved, and blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution (Cat. # SP-6000, Vector Laboratories, Burlingame, CA) for 20 minutes to remove traces of endogenous peroxidase. Subsequently, tissue sections were blocked with Normal Horse Serum Blocking Solution (Cat. # S-2012, Vector laboratories, Burlingame, CA) for 1 hour at room temperature and probed with polyclonal anti-P4HA1 antibody for 1 hour at room temperature. After washes with phosphate-buffered saline (PBS) with Tween, tissue sections were incubated for 1 hour with ImmPRESS HRP Anti-Mouse IgG (Peroxidase) Polymer Detection Kit made in horses (Cat. # MP-7401, Vector Laboratories, Burlingame, CA) as a secondary antibody. After incubation, sections were subjected to washing with PBS with Tween and PBS. Further, ImmPACT DAB Peroxidase (HRP) Substrate (Cat. # SK-4105, Vector Laboratories, Burlingame, CA) was used for color development. Slides were counterstained with hematoxylin solution (Cat. # H-3404, Vector laboratories, Burlingame, CA), dehydrated, and mounted with VectaMount permanent mounting medium (Cat. # H-5000, Vector Laboratories, Burlingame, CA).

Agarwal S., Behring M., Kim H.G., Bajpai P., Chakravarthi B.V., Gupta N., Elkholy A., Al Diffalha S., Varambally S, & Manne U. (2020). Targeting P4HA1 with a Small Molecule Inhibitor in a Colorectal Cancer PDX Model. Translational Oncology, 13(4), 100754.

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Immunohistochemical Analysis of Neurological Markers

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Formalin-fixed dorsolateral prefrontal cortex (dlPFC) sections were immunolabeled with antibodies against GPR39 (anti-GPR39, extracellular domain, antibodies online ABIN1048812), CD68 (anti-CD68, abcam ab955), collagen IV (anti-collagen IV, COL-94, ThermoFisher Scientific MA1-22148), NeuN (anti-NeuN, clone A60, Millipore Sigma MAB377), GFAP (anti-GFAP, clone2E8, abcam ab68428) and SMA was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
(anti-actin, α-smooth muscle, Sigma A2547). Antigen retrieval was carried out by pretreatment with citrate buffer, pH 6 in a steamer for 30 minutes followed by 3% hydrogen peroxide to reduce endogenous peroxidase activity. For both CD68 and collagen IV ImmPRESS HRP anti-mouse IgG (peroxidase) polymer detection kit was used (Vector, MP-7402) and subsequently the reaction visualized using diaminobenzidine. For GPR39 ImmPRESS-AP anti-rabbit IgG (alkaline phosphatase) polymer detection kit was used (Vector, MP-5401) and subsequently the reaction was visualized by Vector blue alkaline phosphatase (Blue AP) substrate kit (Vector, SK-5300). For negative controls the primary antibody was replaced by species-appropriate serum.

Davis C.M., Bah T.M., Zhang W., Nelson J.W., Golgotiu K., Nie X., Alkayed F.N., Young J.M., Woltjer R., Silbert L.C., Grafe M.R., & Alkayed N.J. (2021). GPR39 Localization in Aging Human Brain and Correlation of Expression and Polymorphism with Vascular Cognitive Impairment.

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