Total RNA from cells and tissues were extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA). The concentration and purity of RNA were quantified by Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, 93 USA). Then, total RNA was reverse transcribed to cDNA using HiScript II Reverse Transcriptase SuperMix with gDNA wiper (Vazyme, Nanjing, China) according to the manufacturer's instructions.
Hiscript 2 reverse transcriptase supermix with gdna wiper
Manufactured by Vazyme
Sourced in China
HiScript II Reverse Transcriptase SuperMix with gDNA wiper is a reverse transcription reagent that enables the synthesis of first-strand cDNA from total RNA. It includes a reverse transcriptase enzyme, RNase inhibitor, and a gDNA removal reagent.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hiscript 2 reverse transcriptase supermix with gdna wiper
1
RNA Extraction and cDNA Synthesis
2
Total RNA Extraction and Reverse Transcription
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Total RNA was extracted from cells, tumor and adjacent tissues using the Trizol reagent (Invitrogen, Carlsbad, CA, USA). The concentration of RNA was measured by Nanodrop 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, 93 USA). The RNA was reversely transcribed into cDNA using HiScript II Reverse Transcriptase SuperMix with gDNA wiper (Vazyme, Nanjing, China) according to the manufacturer's instructions.
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