Anti h ras

Total protein was extracted from TT, TPC-1, and ARO cells. Western blotting was employed to detect the expression of target proteins. The primary antibodies, including anti-epidermal growth factor receptor (EGFR), anti-phospho (p)-EGFR, anti-H-RAS, anti-RAF, anti-p-RAF (Ser259), anti-MEK1/2, anti-p-MEK1/2 (Ser217/221), anti-extracellular signal-regulated protein kinase 1/2 (ERK1/2), and anti-p-ERK1/2 (Thr202/Tyr204) antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Anti-B-cell lymphoma-2 (Bcl-2), anti-Bcl-2-associated X protein (Bax), anti-cleaved caspase-3 (cas-3), anti-cleaved poly adenosine diphosphate-ribose polymerase (PARP), and anti-β-actin antibodies were purchased from ProteinTech (Chicago, IL, USA). The horseradish peroxidase-conjugated secondary antibodies were purchased from CST. The results were normalized to the expression level of β-actin. The proteins were visualized using an enhanced chemiluminescence system (Thermo Fisher Scientific, Rockford, IL, USA). The bands were semi-quantified using Image J software.

After treatment for 24 h, total protein was extracted from TPC-1, TT, and ARO cells. Western blotting was performed to detect the expression of target proteins. The primary antibodies, including anti-β-actin, anti-CSE, anti-CBS, anti-3-MST, antisulfide-quinone reductase (SQR), antithiosulfate sulfurtransferase (TST), antiphosphatidylinositol 3-kinase (PI3K), anti-phospho (p)-PI3K (Tyr458/Tyr199), anti-AKT, anti-p-AKT (Ser473), antimammalian target of rapamycin (mTOR), anti-p-mTOR (Ser2448), anti-H-RAS, anti-RAF, anti-p-c-RAF (Ser259), anti-MEK1/2, anti-p-MEK1/2 (Ser217/221), antiextracellular signal-regulated protein kinase 1/2 (ERK1/2), anti-p-ERK1/2 (Thr202/Tyr204), and the horseradish peroxidase-conjugated secondary antibody were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). The results were normalized to the level of β-actin. The reaction was visualized using an enhanced chemiluminescence system (Thermo Fisher Scientific, Rockford, IL, USA). The bands were semiquantified with ImageJ software.