Mouse IL-6 ELISAs (BD Pharmingen, Heidelberg, Germany) and mouse TNF-α ELISAs (R&D Systems, Wiesbaden-Nordenstadt, Germany) were carried out according to the manufacturer’s instructions. Concentrations of cytokines varied between experiments/cell cultures. Qualitative differences or similarities, however, were consistent throughout the study.
Mouse tnf α elisas
Manufactured by R&D Systems
Sourced in Germany
The Mouse TNF-α ELISAs are quantitative sandwich enzyme-linked immunosorbent assays designed for the measurement of mouse tumor necrosis factor alpha (TNF-α) levels in cell culture supernatants, serum, and plasma.
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Lab products found in correlation
2 protocols using mouse tnf α elisas
1
Quantification of Mouse Cytokines
2
Cytokine Secretion in BMMCs
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The supernatants of stimulated BMMCs that were centrifuged for 10 min at 1,000 × g, were used for the detection of secreted cytokines. Mouse IL-6 (BD Pharmingen) and mouse TNF-α ELISAs (R&D Systems) were performed according to the manufacturer’s instructions. Concentrations of cytokines varied between experiments and/or cell cultures due to age of the cells. Qualitative differences or similarities between the genotypes, however, were consistent throughout the study.
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