TRIzol extraction kits (Invitrogen) were utilized to extract the total RNA content from tissues and cells. The primers (Table 1 ) used were synthesized by Takara Biotechnology Ltd. (Dalian, China). The obtained RNA was reverse-transcribed to cDNA using Rever Tra Ace Qpcr RT Master Mix kits (Toyobo Co., Ltd., Tokyo, Japan). Fluorescence qPCR was conducted using SYBR® Premix Ex TaqTM II kits, in accordance with the manufacturers' instructions. PCR conditions were as follows: predenaturation at 94°C for 4 min, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 59°C, extension at 72°C for 1 min, and finally extension at 72°C for 5 minutes. The 2-ΔΔCt method was adopted for expression calculation of genes, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serving as the internal reference.
Rever tra ace qpcr rt master mix kits
Manufactured by Toyobo
Sourced in Japan
The Rever Tra Ace Qpcr RT Master Mix kits are a set of laboratory equipment designed for reverse transcription and real-time PCR analysis. The kits provide a reagent mixture that enables the conversion of RNA into cDNA and the subsequent quantification of the target genes through real-time PCR amplification.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rever tra ace qpcr rt master mix kits
1
Quantitative Gene Expression Analysis
2
RNA Extraction and Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trizol extraction kits (Life technology, Carlsbad, CA, USA) were utilized to separate the total RNA from cells. Then, RNA concentration and purity were determined. Primers of methylation and unmethylation were designed and synthetized by Sangon. RNA was reversely transcribed to cDNA in compliance with the instructions of the Rever Tra Ace Qpcr RT Master Mix kits (Toyobo Co., Ltd., Tokyo, Japan). After that, cDNA was applied to conduct fluorescent qPCR following the instructions of SYBR Premix Ex Tap™ II kits (Takara Bio Inc., Kyoto, Japan). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the internal reference. And 2 -ΔΔCt method was employed for the calculation of relative transcription value in target gene. The primer sequence is shown on Table 2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!