For western blot of endogenous proteins, anti-γH2AX (Cell Signaling Technology (CST), MA #9718, 1:1000), anti-Actin B (CST, MA #4970, 1:1000), anti-p-ATM S1981 (CST, MA #13050, 1:1000), anti-total ATM (CST, MA #2873, 1:1000), anti-total H2AX (CST, MA #2595, 1:1000), anti-RING1A (CST, MA #13069, 1:1000), anti-RING1B (Santa Cruz (SC), CA sc-101109, 1:1000), anti-H2AK119ub1 (CST, MA #8240,1:1000), anti-XPC (SC, CA sc-74410, 1:1000), anti-lamin B (SC, CA sc-6216, 1:1000; SC, CA sc-374015, 1:1000), anti-XPA (SC, CA sc-28353, 1:1000), anti-pRPA32 S33 (Bethyl Laboratories, TX A300–246A-M, 1:1000), anti-RPA32 (CST, MA #2208,1:1000), anti-phospho-Chk1 S345 (CST, MA #2348, 1:1000) and anti-total Chk1 (CST, MA #2360, 1:1000) antibodies were used. For immunofluorescence, anti- γH2AX (CST,MA #9718, 1:100), anti-RING1A (Abcam, CA ab175149, 1:100), anti-RPA32 (CST, MA #2208, 1:100), anti-Rad51 (Novus biological, CO NB100–148, 1:100), anti-H2AK119ub1 (Millipore Sigma, MA 05–678,1:100) and secondary Alexa Conjugate (CST, MA rat #4416, 1:1000, mouse #8890, 1:500, rabbit #4412, 1:1000 and, rabbit #8889,1:500) antibodies were used.
Anti phospho chk1 s345
Manufactured by Fortis Life Sciences
The Anti-phospho-Chk1 S345 is a primary antibody that recognizes the phosphorylated form of the Chk1 protein at serine 345. Chk1 is a crucial regulator of the cell cycle and DNA damage response pathways. The phosphorylation of Chk1 at serine 345 is an important event in the activation of Chk1 and the cellular response to DNA damage.
Automatically generated - may contain errors
Lab products found in correlation
Anti xpc, by Fortis Life Sciences (2 mentions)
Anti h2ak119ub1, by Fortis Life Sciences (2 mentions)
Anti lamin b, by Fortis Life Sciences (2 mentions)
Anti xpa, by Fortis Life Sciences (2 mentions)
Anti ring1a, by Abcam (2 mentions)
Anti rpa32, by Novus Biologicals (2 mentions)
Anti rpa32, by Fortis Life Sciences (2 mentions)
Anti rad51, by Novus Biologicals (2 mentions)
Anti prpa32 s33, by Fortis Life Sciences (2 mentions)
Anti h2ak119ub1, by Merck Group (2 mentions)
Alexa conjugate, by Merck Group (2 mentions)
Anti γh2ax, by Cell Signaling Technology (1 mentions)
Anti γh2ax, by Abcam (1 mentions)
Anti gh2ax, by Cell Signaling Technology (1 mentions)
2 protocols using anti phospho chk1 s345
1
Western Blot Analysis of DNA Damage Proteins
2
Comprehensive Western Blot and Immunofluorescence Analysis of DNA Damage Response Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For western blot of endogenous proteins, anti-gH2AX (Cell Signaling Technology (CST), MA #9718, 1:1000), anti-Actin B (CST, MA #4970, 1:1000), anti-p-ATM S1981 (CST, MA #13050, 1:1000), anti-total ATM (CST, MA #2873, 1:1000), anti-total H2AX (CST, MA #2595, 1:1000), anti-RING1A (CST, MA #13069, 1:1000), anti-RING1B (Santa Cruz (SC), CA sc-101109, 1:1000), anti-H2AK119ub1 (CST, MA #8240,1:1000), anti-XPC ( SC, CA sc-74410, 1:1000), anti-lamin B (SC, CA sc-6216, 1:1000 ; SC, CA sc-374015, 1:1000), anti-XPA (SC, CA sc-28353, 1:1000), anti-pRPA32 S33 (Bethyl Laboratories, TX A300-246A-M, 1:1000), anti-RPA32 (CST, MA #2208,1:1000), antiphospho-Chk1 S345 (CST, MA #2348, 1:1000) and anti-total Chk1 (CST, MA #2360, 1:1000) antibodies were used. For immunofluorescence, anti-gH2AX (CST,MA #9718, 1:100), anti-RING1A (Abcam, CA ab175149, 1:100), anti-RPA32 (CST, MA #2208, 1:100), anti-Rad51 (Novus biological, CO NB100-148, 1:100), anti-H2AK119ub1 (Millipore Sigma, MA 05-678,1:100) and secondary Alexa Conjugate (CST, MA rat #4416, 1:1000, mouse #8890, 1:500, rabbit #4412, 1:1000 and, rabbit #8889,1:500) antibodies were used.
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