Monoclonal mouse anti occludin

Manufactured by Zymo Research

The Monoclonal mouse anti-occludin is an antibody that binds to the occludin protein, a component of tight junctions in epithelial and endothelial cells. This antibody can be used in various research applications to detect and analyze the expression and localization of occludin.

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Lab products found in correlation

Anti mouse alexa 633, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal, by Santa Cruz Biotechnology (1 mentions) Rabbit anti β actin, by Abcam (1 mentions) Polyclonal rabbit anti ang1, by Abcam (1 mentions)

2 protocols using monoclonal mouse anti occludin

3D Co-culture and Tight Junction Staining

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Human macrophages or dendritic cells were pre-stained with 2 mg/ml of 6-carboxyfluorescein diacetate before they were used in the co-culture. The 3D co-culture was fixed with 3% paraformaldehyde. Tight junctions were stained with monoclonal mouse anti-occludin (Zymed, San Francisco, CA; 1:200) and Alexa633 anti-mouse (Life Technologies, Darmstadt, Germany; 1:500), nuclei were stained with DAPI (Life Technologies, Darmstadt, Germany). Detailed protocol is described in SI.

Susewind J., de Souza Carvalho-Wodarz C., Repnik U., Collnot E.M., Schneider-Daum N., Griffiths G.W, & Lehr C.M. (2016). A 3D co-culture of three human cell lines to model the inflamed intestinal mucosa for safety testing of nanomaterials. Nanotoxicology, 10(1).

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Western Blot Analysis of Vascular Regulators

Cited 4 times

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Western blot was conducted as in published protocols (Wang et al., 2006 (link)). Briefly, equal amounts of protein were separated in 10% SDS-polyacrylamide gel and transferred to nitrocellulose membranes. The following antibodies were used: polyclonal rabbit anti-Ang1 (1:1000, Abcam)(Ma et al., 2017 (link)), polyclonal rabbit anti-phospho-Tie2 (p-Tie2, 1:1000, Millipore Sigma)(Ghosh et al., 2016 (link)), monoclonal mouse anti-Tie2 (1:1000, Millipore Sigma) (Makinde and Agrawal, 2011 (link)), monoclonal mouse anti-phospho-NFκB p65 (p-NFκB, 1:1000, Cell Signaling Technology)(Ciaraldi et al., 2016 (link)), polyclonal rabbit anti-vascular cell adhesion molecule-1 (VCAM1, 1:500, Santa Cruz)(Mendoza et al., 2004 (link)), polyclonal rabbit-anti-monocyte chemotactic protein-1 (MCP1, 1:1000, Abcam)(Li et al., 2013 (link)), monoclonal mouse anti-occludin (1:500, Zymed)(Ni et al., 2017 (link)) and rabbit anti-β-actin (1:10000, Abcam)(Miscianinov et al., 2018 (link)). β-actin were determined as a loading control. The density of protein bands were Quantified using Scion Image analysis program (Scion Image).

Wang L., Chopp M., Szalad A., Lu X., Lu M., Zhang T, & Zhang Z.G. (2018). Angiopoietin-1/Tie2 Signaling Pathway Contributes to the Therapeutic Effect of Thymosin β4 on Diabetic Peripheral Neuropathy. Neuroscience research, 147, 1-8.

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