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Epicult b basal medium human

Manufactured by STEMCELL
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Sourced in Canada

EpiCult-B Basal Medium (Human) is a sterile, serum-free, and phenol red-free cell culture medium designed for the culture of human epidermal keratinocytes. It contains essential nutrients and growth factors required for the expansion and maintenance of human epidermal keratinocytes in vitro.

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Lab products found in correlation

Epicult b proliferation supplement human, by STEMCELL (2 mentions) Hydrocortisone, by Maclin Biochemical Technology (1 mentions) Heparin, by STEMCELL (1 mentions) Heparin, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions)

Close spelling variants of this product

Epicult-B Basal medium Epicult Basal Medium EpiCult-B medium Human EpiCult-B EpiCult-B

2 protocols using epicult b basal medium human

1

Mammosphere Formation Assay

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The HCC1806 and MDA-MB-231 cells were plated in ultra-low attachment 96-well plates with EpiCult-B Basal Medium (Human) (Stem Cell Technologies, BC, Canada) and EpiCult-B Proliferation Supplement (Human) (Stem Cell Technologies, BC, Canada) with hydrocortisone (H811182, Macklin, China) and heparin (Stem Cell Technologies, BC, Canada). The mammosphere formation efficiency was calculated after 10–14 days.
Guo L., Ke H., Zhang H., Zou L., Yang Q., Lu X., Zhao L, & Jiao B. (2022). TDP43 promotes stemness of breast cancer stem cells through CD44 variant splicing isoforms. Cell Death & Disease, 13(5), 428.
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2

Mammosphere Formation Assay in 4T1 and TN1 Cells

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A total of 3×104 4T1 or TN1 cells expressing with or without USP22 sgRNA were plated into ultralow-attachment 6-well plates (Corning, Cat#3471), and maintained in EpiCult-B Basal Medium (Human) (Stem Cell Technologies, BC, Canada) and EpiCult-B Proliferation Supplement (Human) (Stem Cell Technologies, BC, Canada), and supplemented with 2 U/mL heparin and 0.5 mg/mL hydrocortisone (Sigma H0135). After 10 days culture, the spheres were pictured, and the number of spheres in each group were counted.
Liu K., Gao Q., Jia Y., Wei J., Chaudhuri S., Wang S., Tang A., Mani N., Iyer R., Cheng Y., Gao B., Lu W., Sun Z., Liu H, & Fang D. (2023). Ubiquitin-specific peptidase 22 controls integrin-dependent cancer cell stemness and metastasis. Research Square.
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