Smmc 7721

Human hepatic carcinoma cell lines SMMC-7721 and HCCLM3 were purchased from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China). SMMC-7721 cells were cultured in RPMI-1640 medium, and HCCLM3 cells were cultured in DMEM. The media were supplemented with 10% fetal bovine serum together with 1% penicillin and streptomycin (P/S). The cells were cultured under standard conditions of 5% CO
₂ at 37°C.

The human hepatoma cell line HepG2 and SMMC-7721 were purchased from Zhongqiao Xinzhou Biotechnology Co., Ltd. and Biowing Biotechnology, Co., Ltd., respectively (Shanghai, China). These cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, United States) supplemented with 10% fetal bovine serum (FBS; Gibco, United States), 100 μg/mL streptomycin (Hyclone, United States), and 100 U/mL penicillin (Hyclone, United States) in a humidified incubator with 5% CO₂ at 37°C. Details of the cell genetic quality identification test report were shown in Supplementary Files 1, 2. MC-LR (purity > 95%) was purchased from the Beijing Solarbio Science & Technology, Co., Ltd., Beijing, it was dissolved in complete DMEM medium to a storage concentration of 100 μM, stock at −20°C until use.

SK-HEP1(catalog number, #ZQ0030), HepG2 (#ZQ0022), Hep3B (#ZQ0024), Huh7 (#ZQ0025), SMMC-7721 (#ZQ0029), HCCLM3 (#ZQ0023), and LO2 (#ZQ0031) were purchased from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Hyclone, CA, USA) or RIPM1640 medium supplemented with fetal bovine serum (FBS; Gibco, CA, USA) and penicillin/streptomycin at 37 °C under the condition of 5% CO2. Human FEN1 cDNA was amplified by PCR and cloned into the pTSB02-GFP-PURO vector constructed by Transheep (Shanghai, China). siRNA Smartpool targeting FEN1 was designed and constructed by Dharmocon (NY, USA). Plasmids or siRNAs were transfected with Lipofectamine 3000 reagent (Invitrogen, CA, USA) according to the manufacturer's instructions. Cells transfected with pTSB02-GFP-PURO plasmids were selected with puromycin (5 μg/mL, Sigma-Aldrich, CA, USA) for 30 days to obtain stably transfected cells.