Cell lysates were prepared with RIPA buffer (Beyotime). Protein concentration was determined using a BCA Protein Assay kit (Pierce). Samples were separated by SDS‐PAGE, blotted onto polyvinylidene fluoride membranes, and probed with primary antibodies, followed by horseradish peroxidase–conjugated goat antirabbit IgG or goat antimouse IgG (Boster Bio Tec). β‐actin was used as a loading control. The primary antibodies included rabbit antihuman EVL (1:50; Santa Cruz Biotechnology), mouse antihuman endoglin (1:800; BD Biosciences), rabbit anti–phosphorylated Smad1/5 (1:1000; Cell Signaling), rabbit anti–phosphorylated Smad2/3 (1:500; Santa Cruz Biotechnology), rabbit anti–phosphorylated Akt (Ser 473, 1:800; Cell Signaling), rabbit anti‐Akt (1:800; Cell Signaling), rabbit anti–phosphorylated ERK (1:1000; Cell Signaling), rabbit anti‐ERK (1:1000; Cell Signaling), mouse anti‐β‐actin (1:1000; Sigma‐Aldrich), mouse anti‐CD31 antibody (1:1000; Abcam), rabbit anti–α‐smooth muscle actin antibody (1:200; Abcam), mouse anti–β‐catenin antibody (1:1000; Millipore), rabbit anti‐vimentin antibody (1:1000; Abcam). Membranes were developed using an enhanced chemoluminescence system (Clinx Science Instruments).
Rabbit anti α smooth muscle actin antibody
Manufactured by Abcam
Sourced in United States
Rabbit anti–α‐smooth muscle actin antibody is a primary antibody that specifically recognizes the alpha-smooth muscle actin protein. It can be used for the detection and localization of alpha-smooth muscle actin in various cell and tissue types.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemoluminescence system, by Clinx (1 mentions)
Rabbit anti phosphorylated erk, by Cell Signaling Technology (1 mentions)
Rabbit anti erk, by Cell Signaling Technology (1 mentions)
Rabbit anti akt, by Cell Signaling Technology (1 mentions)
Anti mouse cd31 antibody, by Abcam (1 mentions)
Rabbit anti phosphorylated akt ser 473, by Cell Signaling Technology (1 mentions)
Mouse anti β catenin antibody, by Merck Group (1 mentions)
Rabbit anti vimentin antibody, by Abcam (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Rabbit anti collagen 1 antibody, by Abcam (1 mentions)
2 protocols using rabbit anti α smooth muscle actin antibody
1
Protein Expression and Signaling Pathways Analysis
2
Fibroblast and Myofibroblast Characterization
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Isolated cells underwent further characterisation by staining for fibroblast markers in addition to LacZ. Immunofluorescence was conducted by using a rabbit anti-collagen I antibody (Abcam, Cambridge, MA, USA) to stain for collagen type 1-producing fibroblasts and rabbit anti-α-smooth muscle actin antibody (Abcam) for myofibroblasts. Secondary antibody goat anti-rabbit IgG Alexa Fluor 488 (Molecular Probes, Eugene, OR, USA) was used. Slides were viewed by using a fluorescence microscope at the Centre for Microscopy, Characterization and Analysis at the University of Western Australia.
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