Male wistar rats crl wi wu

Male Wistar rats (Crl:WI(WU)) were obtained from Charles River (Sulzfeld, Germany). Rats were housed two per cage in an environmentally controlled room with a 12:12-h light–dark circle (light on at 06:00) and ad libitum access to water and standard rat chow (17.7 kJ/g, Ssniff diet R/M-H, V1535 with 18% (w/v) crude protein, 4.7% sugar, and 3.5% crude fat) (Ssniff, Soest, Germany). The rats, including their metabolic characterization, were made available by Sanofi Pharma Deutschland GmbH (Frankfurt am Main, Germany). Blood and serum samples were collected as reported previously [33 (link)].

Male Wistar rats (Crl:WI(WU) were obtained from Charles River (Sulzfeld, Germany). They were housed two per cage in an environmentally controlled room with a 12:12-h light–dark circle (light on at 06:00) and ad libitum access to food and water. Standard rat chow (17.7 kJ/g, Ssniff diet R/M-H, V1535 with 18% crude protein, 4.7% sugar, and 3.5% crude fat; Ssniff, Soest, Germany) was used for 14 weeks. Thereafter, populations of 12 rats (280–320 g) were treated (5 h) with a single bolus administration of Rimonabant (30 mg/kg, p.o.) or vehicle, each, under free access to food. Following terminal isoflurane anesthesia, epididymal adipocytes were prepared by collagenase digestion. All experimental procedures were conducted in accordance with the German Animal Protection Law (paragraph 6) and corresponded to international animal welfare legislation and rules.

Male Wistar rats (Crl:WI(WU)) were obtained from Charles River (Sulzfeld, Germany). See Supplementary Materials.