Chemicals, reagents and plasmids were obtained from the following: Ficoll–Paque Premium (GE Healthcare), Clini-MACS CD3 reagents and CD14 microbeads (Miltenyi Biotech), Blebbistatin, ionomycin, apyrase and PMA (Sigma-Aldrich), brefeldin and monesin (Pharmingen), nonmuscle actin and ATP (Cytoskeleton), CellTracker Orange (Life Technologies), anti–UNC-45A (Enzo Life Sciences), anti-perforin, anti–CD3-PerCP, anti–CD56-allophycocyanin and anti–CD107a-PE (BD Biosciences), anti-NMIIA (Covance), anti–p-NMIIA H chain (Ser1943) (Cell Signaling), anti-NMIIA L chain (Ser20) (Abcam), anti-lysosomal associated membrane protein 1 (LAMP-1), anti-actin (Sigma), PE-conjugated anti-perforin (BD Pharmingen), mouse monoclonal anti-β actin for immunofluorescence (IF) (Sigma), Alexa Fluor 647–conjugated donkey anti-mouse IgG, Texas red goat anti-mouse IgG, Texas red-goat anti-rabbit IgG, FITC-donkey, and anti-mouse IgG (Jackson Immunoresearch Laboratories).
Anti cd56 allophycocyanin
Manufactured by BD
Sourced in United States
Anti–CD56-allophycocyanin is a fluorescently-labeled antibody that binds to the CD56 cell surface antigen. CD56 is expressed on natural killer cells and a subset of T cells. This product can be used to identify and isolate these cell populations in flow cytometry and cell sorting applications.
Automatically generated - may contain errors
Lab products found in correlation
Celltracker orange, by Thermo Fisher Scientific (1 mentions)
Anti cd3 percp, by BD (1 mentions)
Anti cd107a pe, by BD (1 mentions)
Ficoll paque premium, by GE Healthcare (1 mentions)
Goat anti mouse igg texas red, by Jackson ImmunoResearch (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Anti actin, by Merck Group (1 mentions)
Blebbistatin, by Merck Group (1 mentions)
Alexa fluor 647 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Texas red goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Anti unc 45a, by Enzo Life Sciences (1 mentions)
Anti perforin, by BD (1 mentions)
Anti cd3 fitc, by BD (1 mentions)
Anti cd4 phycoerythrin, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Anti cd16 phycoerythrin, by BD (1 mentions)
Anti cd8 allophycocyanin, by BD (1 mentions)
2 protocols using anti cd56 allophycocyanin
1
Isolating and Characterizing Cytolytic Cells
2
Peripheral Blood Lymphocyte Subset Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The percentage of CD3+ T cells, CD3+CD8+ T cells, and CD3−CD16+CD56+ lymphocytes were detected by peripheral blood FCM (Becton Dickinson, Franklin Lakes, NJ, USA). Murine anti-human polyclonal antibodies were used, including anti-CD3-FITC, anti-CD4-phycoerythrin, anti-CD8-allophycocyanin, anti-CD16-phycoerythrin, and anti-CD56-allophycocyanin (BD Biosciences, San Diego, CA, USA). The lymphocytes were delineated by adequate forward and sidelight scatters. CellQuest software (BD Biosciences) was used to analyze the data. The peripheral blood ALC was obtained from the hematology laboratory records, and the percentages of the subtypes were determined using FCM; ACD4C refers to the CD3+CD4+ lymphocyte count, ACD8C refers to the CD3+CD8+ lymphocyte count, and ANKC refers to the CD16+CD56+ lymphocyte count in peripheral blood (15 (link)).
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