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2 protocols using octanal

1

Comprehensive Aldehyde Compound Procurement

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CS was
purchased from Molekula (Munich, Germany).
Propanal, butanal, trans-2-hexenal, octanal, decanal,
and undecylenic aldehyde were purchased from Acros Organics (Geel,
Belgium). Hexanal, dodecanal, acetic acid, and pyrene were purchased
from Sigma-Aldrich (Steinheim, Germany). Double deionized water (DDW)
was obtained by mechanically filtering them through a Treion TS1173
column. Hexane and toluene were purchased from Bio-lab (Ashkelon,
Israel). Deuterated solvents for NMR analysis (D2O, AcOH-d4) were purchased from Armar Chemicals (Döttingen,
Switzerland). Gas chromatography gases (helium, compressed air, nitrogen,
and hydrogen) were purchased from Gordon Gas and Chemicals (Tel Aviv,
Israel) at a 99.999% grade. All the reagents, solvents, and gases
were used without further purification.
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2

Purification and Characterization of Biomolecules

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Materials BLG (97%) was provided by the Food and Bioprocess Engineering group at the Technical University of Munich. 39 LSZ (495%) was purchased by VWR, and BSA (498%) by Sigma-Aldrich. The proteins were dissolved in a pH 7 phosphate buffer (10 mM; Milli-Q-water; Na 2 HPO 4 ÁH 2 O, Acros Organics; NaH 2 PO 4 Á2H 2 O, Sigma-Aldrich). n-Hexane (Merck), n-octane (Acros), 1-chlorooctane (Alfa Aesar), MCT (BASF), octanal (Acros), 1-octanol (Sigma Aldrich), and methyl-tert butyl ether (Sigma Aldrich) were purified from polar contaminants with magnesium silicate Florisil (MgOÁSiO 2 , 100-200 mesh, Sigma-Aldrich) as described in the literature. 40 The purity was tested with a droplet profile tensiometer, to ensure the correct and constant interfacial tension to pure buffer. Solutions of 20 mM dithiothreitol (DTT, Merck) were dissolved in 10 mM phosphate buffer at pH 7.
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