Anti s opsin

Polyclonal Vps34 antibody was obtained from Cell Signaling (Danvers, MA, USA). Polyclonal antisera to the p85α regulatory subunit of class I PI3K were obtained from Upstate Biotechnology, Inc. (Lake Placid, NY, USA). The goat secondary antibody was procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-red/green cone opsin (M-opsin), anti-cone arrestin, anti-S-opsin, and rabbit and mouse secondary antibodies were obtained from Millipore (Billerica, MA, USA). Mouse monoclonal anti-Cre antibody suitable for immunohistochemistry was purchased from Abcam (Cambridge, MA, USA). Monoclonal 1D4 rhodopsin antibody was a kind gift from Dr. James F. McGinnis (University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA). Peanut agglutinin (PNA) and secondary antibodies were purchased from Vector Laboratories (Burlingame, CA, USA). 4′,6-diamidino-2-phenylindole (DAPI) used for nuclear staining was procured from Invitrogen Molecular Probes (Carlsbad, CA, USA). The monoclonal anti-arrestin antibody was a kind gift from Dr. Paul Hargrave (University of Florida, Gainesville, FL, USA). The monoclonal glutamine synthetase (GS) antibody was purchased from Abcam (Cambridge, MA, USA). Polyclonal glial fibrillary acidic protein (GFAP) was purchased from Dako (Carpinteria, CA, USA).