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3 protocols using haucl4

1

Synthesis of Multicomponent Nanoparticles

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Iron(III) acetylacetonate (Fe(acac)3), 1,2-hexadecanediol, oleylamine, polyethylene glycol (PEG), oleic acid, benzyl ether, cetyl trimethyl ammonium bromide (CTAB), HAuCl4, NaBH4, l-ascorbic acid, agar and doxorubicin (DOX) were purchased from J&K Scientific Ltd. Glycolide and Lactide were purchased from Purac (Netherlands) and further purified by recrystallization from ethyl acetate for several times. Stannous octoate, polyvinyl alcohol (PVA) and fluorescein diacetate were purchased from Sigma Aldrich. Fast membrane emulsification equipment (FMEM-500 M) and Shirasu porous glass (SPG) membrane were purchased from National Engineering Research Center for Biotechnology (Beijing). The glass membranes were annular cylinders (inner diameter = 8 mm, external diameter = 10 mm, length = 170 mm) with pore sizes of 7.2 μm. All other reagents were purchased from Beijing Chemical Reagents Company and used as received without further purification. Cells were supplied by China Infrastructure of Cell Line Resources.
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2

Surface Functionalization for Biosensing

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3-Aminopropyltrimethoxysilane (APTMS), 2,5-dihydroxybenzoic acid (DHB), Trifluoroacetic acid (TFA), bovine serum albumin (BSA), human serum albumin (HSA), transferring, immunoglobulin G (IgG), Acetonitrile (ACN), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) hydrochloride were purchased from Sigma (St. Louis, Mo, USA). 11-mercaptoundecanoic acid (MUA) was obtained from Aldrich (Milwaukee, WI, USA). N-Hydroxysuccinimide (NHS) was purchased from Acros (New Jersey, USA). HAuCl4 was purchased from J&K Scientific Ltd. (Beijing, China). NaOH, NaCl, HCl, HNO3, absolute ethanol, methanol, ethanolamine (EOA) were of analytical reagent grade from Beijing Chemical Works (Beijing, China). Antibody of human insulin was purchased from the Cell Signaling Technology, Inc. ITO slides (10 Ω/sq) were purchased from Kaivo Electronic Components Co., Ltd. (Zhuhai, China). All reagents and solvents were used as received. Triply distilled water was used for the preparation of all solutions and rinsing.
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3

Synthesis of Mercaptocyclodextrin Gelatin Hydrogel

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A solution of 200 ​mg Gel-SH in 1.3 ​mL of PBS was combined with 50 ​mg of S-β-CD (Macklin, Shanghai, China) with a magnetic stirrer. Subsequently, 7 ​mg of HAuCl4 (J&K Scientific LLC., San Jose, CA, USA) in 350 ​μL of PBS was added dropwise to make mercaptocyclodextrin gelatin (Gel-β-CD) hydrogel.
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