Trps1 antibody

CHD4 and his-tag antibodies were acquired from Abcam. The TRPS1 antibody was purchased from R&D Systems. β-Actin antibody and anti-mouse secondary antibody were purchased from Proteintech. The ΔNp63 antibody was bought from Abways Technology. Anti-rabbit secondary antibody and anti-goat secondary antibody were purchased from Santa Cruz Biotechnology.
For western blotting, protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a polyvinylidene difluoride membrane, and immunoblotted with antibodies. Blots were developed with enhanced chemiluminescence western blotting reagent (Pierce/Thermo Scientific).

Antibodies for HDAC1–6 and 53BP1 were obtained from Cell Signaling Technology (Beverly, MA). TRPS1 antibody was purchased from R&D Systems (Minneapolis, MN). HDAC9 antibody was purchased from Abcam (Cambridge, MA). H3 and H4 antibodies were obtained from EMD Millipore (Billerica, MA). Ac-H3K9, ac-H3K18 and ac-H4K16 antibodies were purchased from Active Motif (Carlsbad, CA). P53 antibody and anti-rabbit and anti-goat secondary antibodies were purchased from Santa Cruz Biotechnology. β-actin antibody and anti-mouse secondary antibody were purchased from Proteintech (Chicago, IL). For western blotting, protein lysates were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted with the respective antibodies as indicated above and in the figures. Blots were developed with SuperSignal West Femto Maximum Sensitivity Substrate (Pierce/Thermo Scientific, Rockford, IL).