3h para aminohippuric acid

Manufactured by PerkinElmer

Sourced in United States

[3H]Para-aminohippuric acid is a radiolabeled compound used as a tracer in research applications. It serves as a tool to investigate various biological processes and systems.

Automatically generated - may contain errors

Lab products found in correlation

3h estrone 3 sulfate, by PerkinElmer (4 mentions) Rifampin, by Merck Group (3 mentions) Probenecid, by Merck Group (3 mentions) 3h estradiol 17β d glucuronide, by PerkinElmer (3 mentions) 3h methyl 4 phenylpyridinium, by PerkinElmer (3 mentions) Tetraethylammonium chloride, by Merck Group (2 mentions) Non essential amino acids (neaa), by Merck Group (2 mentions) Valsartan, by Merck Group (2 mentions) Poly d lysine coated 96 well plate, by Corning (2 mentions) Sodium butyrate, by Merck Group (2 mentions) 4 methylumbelliferone, by Merck Group (1 mentions) Catalposide, by Merck Group (1 mentions) 3h digoxin, by PerkinElmer (1 mentions) Hank s balanced salt solution hbss, by Corning (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Hank s balanced salt solution hbss, by Merck Group (1 mentions) Compound k, by Ambu (1 mentions) Berberine, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)

Spelling variants of this product

[3H]-labeled para-aminohippuric acid (2 citations)

4 protocols using 3h para aminohippuric acid

Radioactive Tracer Uptake Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³H]Para-aminohippuric acid (PAH; 0.13 TBq/mmol), [³H]estrone-3-sulfate (ES; 2.12 TBq/mmol), [³H]estradiol-17β-D-glucuronide (EG; 2.22 TBq/mmol), [³H]digoxin (1.103 TBq/mmol), and [³H]methyl-4-phenylpyridinium (MPP⁺; 2.9 TBq/mmol) were purchased from Perkin Elmer Inc. (Boston, MA, USA). Catalposide, tetraethylammonium chloride, probenecid, rifampin, cyclosporin A (CsA), 4-meth-ylumbelliferone, and nonessential amino acids were obtained from Sigma-Aldrich (St Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), poly-D-lysine-coated 24-well plates, and Hank’s balanced salt solution (HBSS) were purchased from Corning-Gentest (Tewksbury, MA, USA). Methanol and acetonitrile (high-performance liquid chromatography-grade) were from Burdick & Jackson Inc. (Muskegon, MI, USA). Sodium butyrate and fumitrogen C (FTC) were obtained from Merck Millipore (Billerica, MA, USA). Other chemicals were of the highest quality available.

Jeong H.U., Kwon M., Lee Y., Yoo J.S., Shin D.H., Song I.S, & Lee H.S. (2015). Organic anion transporter 3- and organic anion transporting polypeptides 1B1- and 1B3-mediated transport of catalposide. Drug Design, Development and Therapy, 9, 643-653.

+ Open protocol

+ Expand

Ginseng Compound Identification and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

RGE was purchased from the Punggi Ginseng Cooperative Association (Youngjoo, Kyungpook, Korea). The ginsenosides Rb1, Rb2, Rc, Rd, Rg1, Rg3, Rh1, Rh2, compound K, Re, PPD, and PPT were purchased from the Ambo Institute (Daejeon, Korea). Berberine, caffeine, valsartan, probenecid, rifampin, TEA, sodium dodecyl sulfate (SDS), and Hank’s balanced salt solution (HBSS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), non-essential amino acids, and poly-D-lysine-coated 96-well plates were purchased from Corning Life Sciences (Woburn, MA, USA). [³H]Methyl-4-phenylpyridinium ([³H]MPP⁺; 2.9 TBq/mmol), [³H]para-aminohippuric acid ([³H]PAH; 0.13 TBq/mmol), [³H]estrone-3-sulfate ([³H]ES; 2.1 TBq/mmol), and [³H]estradiol-17β-d-glucuronide ([³H]EG; 2.2 TBq/mmol) were purchased from Perkin Elmer Inc. (Boston, MA, USA). Acetonitrile, methanol, and water were obtained from Fisher Scientific Co. (Fair Lawn, NJ, USA). All other chemicals and solvents were of reagent or analytical grade.

Jeon J.H., Lee S., Lee W., Jin S., Kwon M., Shin C.H., Choi M.K, & Song I.S. (2020). Herb–Drug Interaction of Red Ginseng Extract and Ginsenoside Rc with Valsartan in Rats. Molecules, 25(3), 622.

+ Open protocol

+ Expand

Transporter Uptake Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rosmarinic acid, probenecid, furosemide, valsartan, tetraethylammonium chloride, rifampin, cyclosporin A, Hank’s balanced salt solution (HBSS), sodium butyrate, and non-essential amino acids were purchased from Sigma-Aldrich (St. Louis, MO, USA). [³H]Para-aminohippuric acid (0.13 TBq/mmol), [³H]estrone-3-sulfate (2.12 TBq/mmol), [³H]estradiol-17β-D-glucuronide (2.22 TBq/mmol), [³H]taurocholate (0.57 TBq/mmol), and [³H]methyl-4-phenylpyridinium (2.9 TBq/mmol) were purchased from Perkin Elmer Inc. (Boston, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), poly-D-lysine-coated 24-well plates, and poly-D-lysine-coated 96-well plates were purchased from Corning-Gentest (Tewksbury, MA, USA). Other chemicals were of the highest quality available.
HEK293-OAT1, -OAT3, -OATP1B1, -OATP1B3, -OCT1, -OCT2, and NTCP cells (HEK293 cells transiently overexpressing OAT1, OAT3, OATP1B1, OATP1B3, OCT1, OCT2, and NTCP transporters, respectively) and HEK293-mock cells were purchased from Corning Life Sciences (Woburn, MA, USA).

Kang Y.J., Lee C.H., Park S.J., Lee H.S., Choi M.K, & Song I.S. (2021). Involvement of Organic Anion Transporters in the Pharmacokinetics and Drug Interaction of Rosmarinic Acid. Pharmaceutics, 13(1), 83.

+ Open protocol

+ Expand

HEK293 Cells for Transporter Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human embryonic kidney (HEK) 293 cells transfected with OCT2, OAT1, and OAT3 were obtained from Dr. Kathleen Giacomini (University of California, San Francisco, CA). HEK293 cells transfected with OAT2-variant 1 were obtained from Dr. Ryan Pelis (Dalhousie University, Halifax, Canada). HEK293 cells transfected with MATE1/2K were obtained from Dr. Katsuhisa Inoue (Nagoya City University, Japan). All the test compounds were obtained from the Pfizer chemical inventory system or procured from Sigma-Aldrich (St. Louis, MO). Biocoat poly-D-lysine 48-well and 96-well plates were obtained from Corning Inc. (Corning, NY). Fetal bovine serum was purchased from Sigma-Aldrich (St. Louis, MO). Dulbecco's modified Eagle medium (DMEM), hygromycin B, gentamicin, and sodium pyruvate were obtained from Gibco Life Technologies (Waltham, MA). Hank's balanced salt solution and HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid) were obtained from Lonza Inc. (Allendale, NJ). 14 C-creatinine and 14 Cmetformin were purchased from Moravek Biochemical Inc. (Brea, CA). 3 H-para aminohippuric acid, 3 H-cyclic guanosine monophosphate, and 3 H-estrone 3-sulfate were purchased from Perkin Elmer Life Sciences (Waltham, MA).

Mathialagan S., Rodrigues A.D, & Feng B. (2017). Evaluation of Renal Transporter Inhibition Using Creatinine as a Substrate In Vitro to Assess the Clinical Risk of Elevated Serum Creatinine. Journal of pharmaceutical sciences, 106(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!