For the detection of CD107a, spleen cells of immunized mice were incubated with 50 µM of each peptide for 6 h at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 µg of FITC-conjugated anti-mouse CD107a MAb (BioLegend). Cells were then stained with PE-Cy5-conjugated anti-mouse CD8 MAb (BioLegend). For examination of the CD69 marker, spleen cells of immunized mice were stimulated with 50 µM of each peptide for 4 h at 37°C. Cells were then stained with PE-conjugated anti-mouse CD69 MAb (BioLegend) and FITC-conjugated anti-mouse CD8 MAb. Stained cells were analyzed by flow cytometry (FACSCanto II, BD Biosciences).
Pe cy5 conjugated anti mouse cd8 mab
Manufactured by BioLegend
PE-Cy5-conjugated anti-mouse CD8 MAb is a monoclonal antibody that specifically binds to the CD8 antigen expressed on the surface of mouse T cells. The antibody is conjugated with the fluorescent dye PE-Cy5, which can be used for flow cytometric analysis.
Automatically generated - may contain errors
2 protocols using pe cy5 conjugated anti mouse cd8 mab
1
Flow Cytometric Analysis of Peptide-Specific T Cells
2
Detecting CD107a in Immunized Mice
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For the detection of CD107a, spleen cells of immunized mice were incubated with 50 μM each peptide for 6 h at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a MAb (BioLegend). Cells were then stained with PE-Cy5-conjugated anti-mouse CD8 MAb (BioLegend), and were analyzed by flow cytometry (FACSCanto II, BD Biosciences).
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