NIH3T3 fibroblasts (ATCC® CRL-1658™, ATCC Inc., Manassas, Virginia), a water-soluble tetrazolium assay kit (WST)-8 (Dojindo Molecular Technologies Inc., USA), and a single test vial (STV) LAL test kit (Cape Cod Inc., East Falmouth, USA) were used. Male white mice, 8 weeks old with an average weight of 30–35 grams, were obtained from the Pharmacology Laboratories at Universitas Padjadjaran, Indonesia. Alloxan monohydrate (Sigma Aldrich, USA), Mallory-azan, Dulbecco’s modified Eagle’s medium (GibcoTM DMEM, Fisher Scientific, England), fetal bovine serum (FBS) (Sigma Aldrich, USA), trypan blue solution (Sigma Aldrich, USA), penicillin-streptomycin (PSM) (GibcoTM DMEM, Fisher Scientific, England), trypsin serine protease enzyme (Sigma Aldrich, USA), trichloroacetic acid (TCA) 20% (Sigma Aldrich, USA), alcohol 70% (Sigma Aldrich, USA), and Valisanbe® injection (PT, Sanbe Farma, Indonesia) were used in this study.
Trypsin serine protease enzyme
Manufactured by Merck Group
Sourced in United Kingdom, United States
Trypsin is a serine protease enzyme that catalyzes the hydrolysis of peptide bonds, primarily at the carboxyl side of arginine and lysine residues. It is a widely used enzyme in various laboratory applications, including cell culture, protein purification, and sample preparation for mass spectrometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Trypan blue solution, by Merck Group (2 mentions)
Alloxan monohydrate, by Merck Group (1 mentions)
Wst 8, by Dojindo Laboratories (1 mentions)
Atcc crl 1658, by American Type Culture Collection (1 mentions)
Gibco dmem, by Thermo Fisher Scientific (1 mentions)
Tetracycline hydrochloride, by Merck Group (1 mentions)
Amphotericin b solution, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Gold 3 chloride trihydrate, by Merck Group (1 mentions)
Resazurin sodium salt, by Merck Group (1 mentions)
2 protocols using trypsin serine protease enzyme
1
Alloxan-Induced Diabetes Model in Mice
2
Cytotoxicity Evaluation of Gold Nanoparticles
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Gold (III) chloride trihydrate (HAuCl4·3H2O), sodium hydroxide (NaOH), fetal bovine serum (FBS), penicillin/streptomycin, Resazurin sodium salt, dimethyl sulfoxide (DMSO), trypsin serine protease enzyme, Trypan Blue solution, tetracycline hydrochloride, and Amphotericin B solution were purchased from Sigma-Aldrich (Louis, MO, USA). The PC-3 prostate cancer cell line and the T24 bladder cancer cell lines were purchased from the Cell Bank of Rio de Janeiro (BCRJ). Dulbecco’s Modified Eagle Medium (DMEM), Roswell Park Memorial Institute medium (RPMI) and BD Brain Heart Infusion (BHI) agar were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA).
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