URB597 (Sigma Aldrich) and URB937 (Cayman Chemical) were dissolved in 18:1:1 normal saline:PEG400:Tween80 (Sigma Aldrich) and administered to mice via intraperitoneal injection at a dosage of 0.1–1mg/kg bodyweight four hours prior to behavioral testing or sacrifice.
Peg400 tween80
Manufactured by Merck Group
Sourced in United States
PEG400:Tween80 is a lab equipment product. It is a mixture of polyethylene glycol 400 (PEG400) and polysorbate 80 (Tween80). This product is used as a solvent and surfactant in various laboratory applications.
Automatically generated - may contain errors
2 protocols using peg400 tween80
1
Cannabinoid compounds administration
2
Developing CIPN and HSR Models Using Paclitaxel and DF3966A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For CIPN model, paclitaxel solution (Onatax®, 100 mg/16.7 ml; cod 018155) was kindly provided by Prof. Thiago M. Cunha (Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Brazil). For the in vivo experiments, DF3966A (Dompé farmaceutici S.p.A., L’Aquila, Italy) was dissolved in water (30 mg/10 ml), and orally given at a dose of 30 mg/kg every 12 h for 14 days during the induction phase of CIPN. On days 1, 3, 5, and 7, DF3966A was administrated 1 h after paclitaxel.
For HSR model, paclitaxel (Selleckchem, USA, cod. S1150) was intravenous administrated at 20 mg/kg (0.1 mL/Kg) dissolved in Cremophor EL (polyoxyl 35 castor oil, Sigma-Aldrich, cod. 238470) or in PEG400/TWEEN80 (Sigma-Aldrich). Vehicle DF3966A (saline) and DF3966A (30 mg/kg, os) were administrated 3 h before paclitaxel, while vehicle Avacopan (PEG-400/solutol-HS15 70:30, 5 mL/kg [29 (link)]) and Avacopan (30 mg/kg, os) were administrated 1 h before paclitaxel.
For HSR model, paclitaxel (Selleckchem, USA, cod. S1150) was intravenous administrated at 20 mg/kg (0.1 mL/Kg) dissolved in Cremophor EL (polyoxyl 35 castor oil, Sigma-Aldrich, cod. 238470) or in PEG400/TWEEN80 (Sigma-Aldrich). Vehicle DF3966A (saline) and DF3966A (30 mg/kg, os) were administrated 3 h before paclitaxel, while vehicle Avacopan (PEG-400/solutol-HS15 70:30, 5 mL/kg [29 (link)]) and Avacopan (30 mg/kg, os) were administrated 1 h before paclitaxel.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!