Rat anti ly6g ab

The expression of RXFP1 and GR (liver, heart, kidney, lung, esophagus) was examined using rabbit anti-RXFP1 Ab (Santa Cruz) and rabbit anti-GR mAb (Cell Signaling Technology, Danvers, MA). Immunostaining signals were visualized with a labeled polymer in the EnVision+ system horseradish-peroxidase kit (Dako, Carpinteria, CA). Liver infiltrating macrophages and neutrophils were detected with rat anti-CD11b Ab (BD Biosciences, San Jose, CA) and rat anti-Ly6G Ab (Bio-Rad, Hercules, CA), respectively. Signals were visualized with secondary Alexa Fluor 488 anti-rat IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (x400).

Formalin-fixed paraffin-embedded liver sections (5μm) were stained with hematoxylin and eosin. The severity of IRI was graded using Suzuki’s criteria.^{21 (link)}The mouse liver infiltrating macrophages and neutrophils were detected with rat anti-CD11b Ab (BD Biosciences, San Jose, CA) and rat anti-Ly6G Ab (Bio-Rad, Hercules, CA), respectively. Signals were visualized with secondary Alexa Fluor 488 anti-rat IgG. Results were scored semi-quantitatively by blindly counting the number of positive cells in 10 HPF/section (x400).