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Hemavet 950

Manufactured by Drew Scientific
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Sourced in United States

The Hemavet 950 is a compact, automated hematology analyzer designed for comprehensive blood cell analysis. It provides accurate and reliable measurements of various blood parameters, including red blood cells, white blood cells, and platelet counts.

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Lab products found in correlation

Edta coated microvette tubes, by Sarstedt (7 mentions) C57bl 6 mice, by Jackson ImmunoResearch (4 mentions) Vacutainer tm tubes, by BD (4 mentions) Advia counter, by Siemens (2 mentions) Serum urea nitrogen, by BioAssay Systems (2 mentions) Serum iron, by Sanofi (2 mentions) Serum erythropoietin, by R&D Systems (2 mentions) B6 sjl boyj cd45.1 recipient, by Jackson ImmunoResearch (2 mentions) Rat d dimer elisa kit, by Abbexa (2 mentions) Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (2 mentions) Anti gr 1 clone rc57bl 6 8c5, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Edta 3k, by BD (2 mentions) Bs 400, by Mindray (2 mentions) Methylcellulose culture, by STEMCELL (2 mentions) Facscanto 2, by BD (2 mentions) Elisa kit, by R&D Systems (2 mentions) Lsrfortessa cell analyzer, by BD (2 mentions) Blood collection tubes, by Sarstedt (1 mentions)

179 protocols using hemavet 950

1

Peripheral Blood and Tissue Histology

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Complete peripheral blood (PB) counts were analyzed using a Drew Scientific Hemavet 950. Briefly, a portion of PB from the cardiac puncture was collected using Microtainer blood collection tubes (BD) with a K2EDTA additive. After thorough mixing within the K2EDTA containing tube, blood was transferred to a 1.5 ml microcentrifuge tube and run on the Drew Scientific Hemavet 950. For tissue histology, tibias and spleens were fixed in formalin, embedded in paraffin blocks, sectioned, and stained with hematoxylin and eosin (H&E).
Varney M.E., Boehm D.T., DeRoos K., Nowak E.S., Wong T.Y., Sen-Kilic E., Bradford S.D., Elkins C., Epperly M.S., Witt W.T., Barbier M, & Damron F.H. (2018). Bordetella pertussis Whole Cell Immunization, Unlike Acellular Immunization, Mimics Naïve Infection by Driving Hematopoietic Stem and Progenitor Cell Expansion in Mice. Frontiers in Immunology, 9, 2376.
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2

Hematological Parameter Evaluation

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Selected hematological parameters were evaluated using a Hemavet 950 automatic hematology analyzer (Drew Scientific, Waterbury, CT). Endpoints analyzed included peripheral erythrocyte and leukocyte counts, leukocyte differentials (lymphocytes, neutrophils, monocytes, basophils, and eosinophils), platelet counts, hematocrit, hemoglobin levels, mean corpuscular hemoglobin (MCH) and hemoglobin concentration (MCHC), mean corpuscular volume (MCP), mean platelet volume (MCV), and platelet distribution width (PDW).
Marrocco A., Meade B.J., Long C.M., Lukomska E., Marshall N.B, & Anderson S.E. (2015). Investigations into the Immunotoxicity and Allergic Potential Induced by Topical Application of N-Butylbenzenesulfonamide (NBBS) in a Murine Model. Journal of toxicology and environmental health. Part A, 78(17), 1122-1132.
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3

Cardiac Puncture Blood Collection

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Blood was collected into blood collection tubes (Sarstedt, # 41.1504.105) by cardiac puncture. Hematocrit and white blood cell (WBC) and platelet counts were measured using a Hemavet instrument (Hemavet 950; Drew Scientific Group) according to the manufacturer’s instructions.
Branche E., Tang W.W., Viramontes K.M., Young M.P., Sheets N., Joo Y., Nguyen A.V, & Shresta S. (2018). Synergism Between the Tyrosine Kinase Inhibitor Sunitinib and Anti-TNF Antibody Protects Against Lethal Dengue Infection. Antiviral research, 158, 1-7.
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4

Comprehensive Blood Analysis Protocol

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Blood was collected from the heart after sacrificing the animal. One hundred microliter of blood was collected in a microtainer containing ethylenediaminetetraacetic acid (BD, Franklin Lakes, NJ, USA) and analyzed using Hemavet 950 (Drew Scientific, Dallas, TX, USA). The hematological parameters studied were as follows: white blood cell count, red blood cell (RBC) count, hemoglobin concentration, hematocrits, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width, platelet (PLT) count, mean PLT volume, percentage of neutrophils, percentage of lymphocytes, percentage of monocytes, percentage of eosinophils, and percentage of basophils.
Thakkar A., Chenreddy S., Thio A., Khamas W., Wang J, & Prabhu S. (2016). Preclinical systemic toxicity evaluation of chitosan-solid lipid nanoparticle-encapsulated aspirin and curcumin in combination with free sulforaphane in BALB/c mice. International Journal of Nanomedicine, 11, 3265-3276.
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5

Blood Collection and Analysis

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Blood was collected into Microvette 500 K3E tubes (Sarstedt). Complete blood counts were measured with a Hemavet 950 veterinary hematology analyzer (Drew Scientific).
Colijn S., Muthukumar V., Xie J., Gao S, & Griffin C.T. (2020). Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis. Disease Models & Mechanisms, 13(1), dmm041962.
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6

Isolation and Analysis of Murine Hematopoietic Cells

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Mice were sacrificed, and tibiae, femurs, sternum, coxae, and vertebral columns were crushed in RPMI-1640 medium (Sigma-Aldrich) supplemented with 2% FCS. For cell sorting, lineage depletion was performed by incubating BM cells with rat monoclonal antibodies targeting CD4 (GK1.5), CD8a (53.6.7), CD11b (M1/70), B220 (RA3-6B2), Gr-1 (RB6.8C5), and Ter119 (Ter119). Subsequently, cells were washed, and lin+ cells were removed using magnetic beads (Dynabeads; Invitrogen). For analysis of PB, four to five drops of blood were collected from the vena facialis into an EDTA-coated tube. Blood cells were analyzed using a hematology system (Hemavet 950; Drew Scientific). For FACS analysis, erythrocytes were lysed with 1 ml ammonium-chloride-potassium lysing buffer.
Uckelmann H., Blaszkiewicz S., Nicolae C., Haas S., Schnell A., Wurzer S., Wagener R., Aszodi A, & Essers M.A. (2016). Extracellular matrix protein Matrilin-4 regulates stress-induced HSC proliferation via CXCR4. The Journal of Experimental Medicine, 213(10), 1961-1971.
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7

Comprehensive Murine Hematology Analysis

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Blood was collected from the retro-orbital sinus into EDTA-coated eppendorf tubes. Complete blood count (CBC) was performed by a HemaVet 950 analyzer (Drew Scientific, Inc., Waterbury, CT). After euthanasia by CO2, BM cells were extracted from bilateral tibiae and femurs, filtered through 95 μM nylon mesh, and counted by a Vi-Cell counter (Beckman Coulter, Miami, FL). BM cells were stained with antibody mixtures on ice for 30 minutes in FBS-supplemented RPMI 1640 (Life Technilogies), and acquired using BD FACSCanto II and BD LSRFortessa flow cytometers operated by FACSDiva software (Becton Dickson, San Diego, CA).
Monoclonal antibodies for murine CD3 (clone 145-2C11), CD4 (clone GK 1.5), CD8 (clone 53–6.72), CD44 (clone IM7), CD48 (clone HM48-1), CD62L (clone MEL-14), CD117 (c-Kit, clone 2B8), CD150 (SLAM, clone TC15-12F12.2), erythroid cells (clone Ter119), granulocytes (Gr1/Ly6-G, clone RB6-8C5), stem cell antigen-1 (Sca-1, clone E13-161), and IFN-γ (clone XMG1.2) were all from BioLegend (San Diego, CA). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-cyanin 5 (PE-Cy5), PE-cyanin 7 (PE-Cy7), allophycocyanin (APC) or brilliant violet 421 (BV421).
Solorzano S., Kim J., Chen J., Feng X, & Young N.S. (2021). Minimal role of interleukin 6 and toll-like receptor 2 and 4 in murine models of immune-mediated bone marrow failure. PLoS ONE, 16(3), e0248343.
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8

Bronchoalveolar Lavage Fluid Analysis

Cited 1 time
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BAL fluid was cataloged by digital photography and BAL optical density at 540 nm (OD540) was measured immediately using 100-µL aliquots. BAL hemoglobin and platelet counts were measured in 1 mL BAL fluid by Hemavet 950 (Drew Scientific) as described in a prior report.54 (link) BAL total protein concentration was determined after centrifugation by Pierce BCA Protein Assay. BAL immunoglobulin M (IgM) was determined following 1:10 dilution according to the manufacturer’s instructions (#E90-101, Bethyl Labs).
Bain W., Olonisakin T., Yu M., Qu Y., Hulver M., Xiong Z., Li H., Pilewski J., Mallampalli R.K., Nouraie M., Ray A., Ray P., Cheng Z., Shanks R.M., St. Croix C., Silverstein R.L, & Lee J.S. (2019). Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury. Blood Advances, 3(3), 432-445.
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9

Platelet Aggregation Assay Protocol

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For aggregation assays, platelets in plasma was prepared and platelet counts were normalized (300,000/µL) using a Hemavet 950 (Drew Scientific, Waterbury, CT). Collagen (3 µg/ml) was monitored by light transmittance (Chrono-log Corp., Havertown, PA)13 (link)17 (link).
Ziu E., Hadden C., Li Y., Lowery CL I.I.I., Singh P., Ucer S.S., Mercado C.P., Gu H.H, & Kilic F. (2014). Effect of serotonin on platelet function in cocaine exposed blood. Scientific Reports, 4, 5945.
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10

Bone Marrow Cell Immunophenotyping by Flow Cytometry

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For flow cytometry, 1–2 × 106 freshly isolated bone marrow cells in 200 μl buffer or 100 μl whole blood were stained with conjugated antibodies on ice for 30 min. Fc receptors were blocked by incubation with anti-mouse CD16/CD32 antibodies for 5 min on ice. After antibody incubation, erythrocytes were lysed by adding 2 ml lysis buffer (150 mM NH4Cl, 10 mM NaHCO3, 1 mM EDTA) for 3 min at room temperature. After a final wash, samples were analysed on a FACSCalibur cytometer (Becton Dickinson). Leukocyte subpopulations were distinguished by light scatter and/or by specific antibodies. Cold Ca2+/Mg2+-free Hanks' balanced salt solution containing 5 mM EDTA and 0.1% human serum albumin (HBSS−/EDTA/HSA) was used unless otherwise specified. Peripheral blood counts were measured using a veterinary haematology analyser (Hemavet 950, Drew Scientific Inc.).
Liu Z., Yago T., Zhang N., Panicker S.R., Wang Y., Yao L., Mehta-D'souza P., Xia L., Zhu C, & McEver R.P. (2017). L-selectin mechanochemistry restricts neutrophil priming in vivo. Nature Communications, 8, 15196.
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