50i microscope

Manufactured by Nikon

Sourced in Japan

The Nikon 50i microscope is a high-performance laboratory microscope designed for a variety of applications. It features a sturdy construction, precise optics, and user-friendly controls. The microscope is capable of providing clear, detailed images for a wide range of sample types.

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Lab products found in correlation

Osteomeasure xp system, by OsteoMetrics (2 mentions) Ds ri1 digital camera, by Nikon (2 mentions) Rm2145, by Leica (1 mentions) Trap staining kit 387 a, by Merck Group (1 mentions) Cover glasses, by Thermo Fisher Scientific (1 mentions) Toluidine blue, by Merck Group (1 mentions) Rotary microtome, by Leica (1 mentions) Citrate buffer, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti p53 antibody, by Santa Cruz Biotechnology (1 mentions) Benchmark xt, by Roche (1 mentions) Hrp aec abc detection ihc kit, by Abcam (1 mentions) Dfc300 camera, by Leica (1 mentions) Y idt drawing tube, by Nikon (1 mentions) Ds fi camera, by Nikon (1 mentions) Dsl2 camera control unit, by Nikon (1 mentions) Cryostat, by Leica (1 mentions)

Close spelling variants of this product

Eclipse 50i light microscope Eclipse 50i optical microscope Eclipse 50i microscope Nikon 50i light microscope Nikon 50i inverted microscope 50i Nikon fluorescence microscope Eclipse 50i fluorescence microscope Nikon Eclipse 50i upright microscope Eclipse 50i fluorescent microscope Nikon 50i epifluorescence microscope

18 protocols using 50i microscope

Apelin Effect on Endothelial Cell Junctions

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HRMECs, pretreated with apelin (0, 1, 10, 100, and 1000 ng/ml) in normal (4.5 mM) or high glucose ECM, were cultured on cover glasses (Fisher, USA) and then fixed with acetone for 10 min [17 (link)]. Primary antibodies were incubated overnight at 4°C. The primary antibodies used for immunofluorescence (IF) staining were rabbit anti-apelin, anti-VE-Cadherin, anti-ZO-1, and anti-occludin (Table 1). After blocking, the sections were washed and then incubated with relevant fluorescence-conjugated secondary antibodies (1 : 1000) (Table 1). The samples were counterstained with DAPI. The images were acquired using a Nikon 50i microscope with NIS-Elements Imaging Software under 200x magnifications.
For mice retina detection, IF frozen sections (8 mm thick) were blocked with 1% BSA (30 min–1 h, RT), and zonula occludens-1 (ZO-1) and occludin (Table 1) were used. For double-labeling IF study, they were then incubated with the relevant fluorescence-conjugated secondary antibodies (Table 1). The samples were counterstained with DAPI. IF was acquired using a Nikon 50i microscope.

Li Y., Bai Y.J., Jiang Y.R., Yu W.Z., Shi X., Chen L., Feng J, & Sun G.B. (2018). Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways. BioMed Research International, 2018, 3242574.

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Adiposity and Pancreatic Islets Analysis

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At the end of week 40, the animals were sacrificed with monosodic pentobarbital (35 mg/kg intraperitoneally), and the adipose tissue was dissected and weighted to determine adiposity percentage. A portion of the pancreas and visceral adipose tissue was fixed in a 10% buffered formalin for 48 h and was embedded in paraffin. Five-micrometer section slices for the pancreas and 20 µm for visceral adipose tissue were obtained with a standard microtome (LEICA RM 2145). The slices were stained with hematoxylin–eosin, and the photomicrographs acquired with the Nikon-50i microscope were analyzed with ImageJ software that quantified the Langerhans islets per 1 cm² and counted the cellularity of each one. Also, it quantified adipocytes per+ 1 cm² and its diameter.

Tapia-Martínez J.A., Franco-Colín M., Blas-Valdivia V, & Cano-Europa E. (2021). The joint effect of congenital hypothyroidism and hypercaloric diet consumption as triggers of type 2 diabetes mellitus. European Thyroid Journal, 11(1), e210050.

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Dynamic Bone Histomorphometry in Mice

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For dynamic bone histomorphometry, mice were intraperitoneally injected with 30 mg/kg calcein 9 days prior to sacrifice and then with 10 mg/kg Alizarin Red at 2 days prior to sacrifice. Tibia were harvested and embedded with methyl methacrylate. Six-micron sections were cut using a Leica SM2500 metallurgical cutting system. For the measurement of in vivo osteoclast activity, tibiae were harvested from 12 week old mice. TRAP staining was performed on histological sections using a Sigma Trap staining kit (387-A). The fluorescently-labeled and TRAP stained images were photographed using a Nikon 50i microscope. Histomorphometric parameters for double labels and osteoclast activity were assessed using an OsteoMeasurexp system (OsteoMetrics Inc, GA, USA).

Ge C., Zhao G., Li B., Li Y., Cawthorn W.P., MacDougald O.A, & Franceschi R.T. (2017). Genetic Inhibition of PPARγ S112 Phosphorylation Reduces Bone Formation and Stimulates Marrow Adipogenesis. Bone, 107, 1-9.

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Histopathological Analysis of Spinal Cord

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All spinal cord tissue sections used here were 5 µm thick. For paraffin-embedded tissue, spinal cords collected from PBS-perfused mice were fixed in 4% paraformaldehyde overnight. Sections were stained with H&E or with LFB for evaluation of inflammation and demyelination, respectively. For immunohistochemical staining, sections were blocked and incubated with primary antibodies and horseradish peroxidase-conjugated secondary antibodies after heat-induced antigen retrieval. Diaminobenzidine was used for detection. Images were captured with a Nikon 50i microscope.

Li S., Wu Y., Yang D., Wu C., Ma C., Liu X., Moynagh P.N., Wang B., Hu G, & Yang S. (2019). Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis. The Journal of Experimental Medicine, 216(11), 2562-2581.

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Evaluating Mouse Bone Dynamics

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For dynamic bone histomorphometry, 12 week old mice were intraperitoneally injected with 30 mg/kg calcein 9 days prior to sacrifice and then with 10 mg/kg Alizarin Red at 2 days prior to sacrifice. Tibia were harvested and embedded with methyl methacrylate. Six micron sections were cut using Leica SM2500 metallurgical cutting system. For the measurement of in vivo osteoclast activity, tibea were harvested from 12 week old mice. TRAP staining was performed on histological sections using the Sigma Trap staining kit (387-A). The fluorescence and TRAP stained images were photographed using a Nikon 50i microscope. Histomorphometric parameters for double labels and osteoclast activity were assessed using an OsteoMeasurexp system (OsteoMetrics Inc, GA, USA).

Ge C., Wang Z., Zhao G., Li B., Liao J., Sun H, & Franceschi R.T. (2016). Discoidin Receptor 2 Controls Bone Formation and Marrow Adipogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 31(12), 2193-2203.

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Rice Spikelet Histological Analysis

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Rice spikelets were sampled at the heading stage and fixed in the formalin–acetic acid–alcohol (FAA) solution (5% formaldehyde, 5% glacial acetic acid, and 50% ethanol), followed by degassing under a vacuum pump for 25 min, and incubation in the FAA solution for at least 24 h. Fixed tissue samples were dehydrated in an ethanol solution series, destained in xylene solution, and embedded in paraffin. Tissue sections (7 μm thick) were cut with a rotary microtome (Leica, Germany) and mounted on a glass slide, followed by treatment with xylene and staining with 0.2% toluidine blue (Sigma, Germany). Sections were photographed under a Nikon 50i microscope (Nikon, Japan).

Chen X., Jiang L., Zheng J., Chen F., Wang T., Wang M., Tao Y., Wang H., Hong Z., Huang Y, & Huang R. (2019). A missense mutation in Large Grain Size 1 increases grain size and enhances cold tolerance in rice. Journal of Experimental Botany, 70(15), 3851-3866.

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Immunohistochemical p53 Assessment

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After antigen retrieval with 0.01 mol/L citrate buffer (pH 6.0; Invitrogen, Carlsbad, CA) in a microwave for 25 min, blocking with hydrogen peroxide for 10 min, and protein blocking for 40 min, 5-mm-thick paraffin-embedded sections fixed on Plus glass slides (Menzel Glaser, Braunschweig, Germany) were processed by using an automated immunostainer (Benchmark-XT; Ventana Medical System, Tucson, AZ) with mouse monoclonal anti-p53 antibody (diluted 1:20; Santa Cruz Biotechnology, Santa Cruz, CA, DO-1). Negative controls consisted of slides processed while omitting the primary antibody. Visualization of the bound primary antibodies was performed with the mouse and rabbit specific HRP/AEC (ABC) Detection IHC Kit (Abcam, Cambridge, UK, ab93705). Sections were then counterstained with Gill hematoxylin, dehydrated, and mounted for microscopic examination. Specimens were examined with a Nikon 50I microscope connected to a DS-RI1 digital camera (Nikon, Tokyo, Japan). Immunohistochemistry nuclear staining was quantified with ImageJ software (National Institutes of Health, Bethesda, Md) by two independent evaluators.

Assaf S., Vodo D., Malovitski K., Mohamad J., Bergson S., Feller Y., Malki L., Sarig O, & Sprecher E. (2022). Up-regulation of ST18 in pemphigus vulgaris drives a self-amplifying p53-dependent pathomechanism resulting in decreased desmoglein 3 expression. Scientific Reports, 12, 5958.

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Quantifying Cardiac Interstitial Fibrosis

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Five micron sections of heart from all of the treatments were stained with picro-Sirus-red for evaluation of interstitial fibrosis. Slides were checked with a Nikon50i microscope and five images were randomly captured from the left ventricle and right ventricle with a cool snapcf camera and auto leveled with Photoshop. Morphometric analysis was performed using MetaVue software. In each image, the areas of hot pink color and their intensities, which are representative of interstitial fibrosis, were quantified. Collagen 1 and 3 were evaluated by immunostaining using specific antibodies for each isoform.^{23 (link)}

Jia G., Habibi J., Bostick B.P., Ma L., DeMarco V.G., Aroor A.R., Hayden M.R., Whaley-Connell A.T, & Sowers J.R. (2014). Uric Acid Promotes Left Ventricular Diastolic Dysfunction in Mice Fed a Western Diet. Hypertension, 65(3), 531-539.

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Microscopic Examination of Tetrapoda

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Manūs (N = 9) and pedes (N = 9) of ethanol-preserved juveniles and adults of T. annularis and of various embryonic stages (N = 3 to 5 for each stage) were examined using a Nikon 50i microscope and Nikon DS-5 M camera head and camera control unit (Nikon, Tokyo, Japan) and a Leica stereo microscope (MZFiii) and Leica DFC300 camera (Leica, Solms, Germany).

Khannoon E.R., Russell A.P, & Tucker A.S. (2015). Developmental mechanisms underlying differential claw expression in the autopodia of geckos. EvoDevo, 6, 8.

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Karyotyping of Epinephelus Hybrids

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Karyotype analyses of each five individuals of E. coioides (the female parent), E. lanceolatus (the male parent), diploid hybrids, and triploid hybrids were operated to infer the chromosomal origin. Karyotype slides were prepared from cultured peripheral blood cells. Detailed processes were conducted as described by Huang et al. (2014) [15 ]; blood was collected and cultured in nutrient solution at 25.5 °C with 5 % CO₂ for 68–72 h, and 0.1 ml colchicines solution was added 3 h before harvest. The cells were then treated with 0.075 M KCl at 37 °C for 30 min. Blood cells were dropped on the cold slides and air-dried at room temperature. Chromosomes were stained by Giemsa solution (pH 6.8) for 30 min, washed in running water, air-dried. Good-quality metaphase spreads images were obtained by a Nikon 50i microscope (Nikon, Japan) for karyotype analyzing. Fine karyotype analysis was carried out as Qin et al. (2014) [8 (link)] had described. Long-arm to short-arm ratios of 1.0–1.7 were classified as metacentric (m), 1.7–3.0 as submetacentric (sm), 3.1–7.0 as subtelocentric (st), and > 7.1 as telocentric (t) chromosomes (Levan et al. 1964) [20 (link)].

Huang W., Qin Q., Yang H., Li S., Hu C., Wang Y., Zhang Y., Liu S, & Lin H. (2016). Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides ♀ × Epinephelus lanceolatus ♂) and their 5S gene analysis. BMC Genetics, 17, 136.

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