Tris hydroxymethyl aminomethane tris

Manufactured by Merck Group

Sourced in United States, Germany, China, Belgium, United Kingdom, Singapore, Italy

Tris(hydroxymethyl)aminomethane (Tris) is a chemical compound commonly used as a buffer in various laboratory applications. It is a white, crystalline solid that acts as a pH regulator, maintaining a stable pH environment in solutions. Tris is known for its versatility in biological, biochemical, and analytical procedures.

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Lab products found in correlation

82 protocols using tris hydroxymethyl aminomethane tris

Lipid Formulation for Photodynamic Therapy

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was obtained from Avanti Polar Lipids (Alabaster, AL). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG, average PEG molecular mass of 2,000 amu), ZnPC (97 % purity), HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), pyridine, and sulforhodamine B (SRB) were acquired from Sigma-Aldrich (St. Louis, MO). Acetic acid (glacial), ethidium bromide, ethylenediaminetetraacetic acid (EDTA), formaldehyde solution (36.5–38 % in water), sodium chloride, and tris(hydroxymethyl)aminomethane (Tris) were obtained from Merck KGaA (Darmstadt, Germany). Agarose was purchased from Gibco-BRL (Paisley, UK) and ethanol was from J.T. Baker (Deventer, the Netherlands).
All lipids were dissolved in chloroform and ZnPC was dissolved in pyridine at a 178-μM concentration. All dissolved lipids were stored under a nitrogen atmosphere at −20 °C.

Weijer R., Broekgaarden M., van Golen R.F., Bulle E., Nieuwenhuis E., Jongejan A., Moerland P.D., van Kampen A.H., van Gulik T.M, & Heger M. (2015). Low-power photodynamic therapy induces survival signaling in perihilar cholangiocarcinoma cells. BMC Cancer, 15, 1014.

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Analytical Grade Chemicals Protocol

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All chemicals were analytical grade. Methanol (MeOH), iron (II)-sulfate-7-hydrate and trichloroacetic acid were obtained from Riedel-de Haën™ (Seelze, Germany) and formic acid, water and acetonitrile (ACN) from Fisher Scientific, OptimaTM (Hampton, NH, USA). Trifluoroacetic acid (TFA), potassium dihydrogen phosphate, tris(hydroxymethyl)aminomethane (Tris) and sodium chloride were bought from Merck (Darmstadt, Germany). Ethanol 96% was bought from Carlo Erba (Peypin, France), gentiopicroside from TransMIT (Hesse, Germany) and potassium chloride from Fluka (Seelze, Germany). Acetylcholinesterase (AChE), acetylcholine iodide (AChI), 5-5′-dithiobis (2-nitrobenzoic acid) (DTNB), HMG-CoA Reductase assay kit, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), butylated hydroxytoluene (BHT), quercetin, rutin and galantamine were obtained from Sigma (Barcelona, Spain). Magnesium chloride hexahydrate, thiobarbituric acid, hydrogen peroxide, sodium hydrogen phosphate and 1-butanol were bought to PanReac (Barcelona, Spain). Sodium hydroxide was obtained from JT Baker, UK and sodium nitroprusside and Griess reagent from VWR Chemicals PL.

Guedes L., Reis P.B., Machuqueiro M., Ressaissi A., Pacheco R, & Serralheiro M.L. (2019). Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies. Molecules, 24(20), 3795.

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DMPC Liposome Formulation Protocol

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1,2-Dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) (purity = 99%) was supplied by Genzyme Pharmaceuticals (Liestal, Switzerland). Methanol and chloroform were of LC grade and were bought from VWR (Darmstadt, Germany). Tris(hydroxymethyl)-aminomethane (Tris) was purchased from Merck (Germany). A 50 mM Tris solution, at pH 7.4, was used to prepare MLV. Fluka (Milan, Italy) supplied Polyoxyethylene-20-cetyl ether (Ceteth-20). Polyoxyethylene-20-isohexadecyl ether (Isoceteth-20) was a kind gift of Bregaglio (Milan, Italy). Polyoxyethylene-20-oleyl ether (Oleth-20), glyceryl oleate (Tegin O, GO), cetyl palmitate (CP), methylchloroisothiazolinone and methylisothiazolinone (Acnibio AC) and imidazolidinyl urea (Kemipur 100) were purchased from ACEF (Fiorenzuola d'Arda, PC, Italy). Idebenone (IDE) was a kind gift of Wyeth Lederle (Catania, Italy). All other reagents were of analytical grade and used as supplied.

Montenegro L., Castelli F, & Sarpietro M.G. (2018). Differential Scanning Calorimetry Analyses of Idebenone-Loaded Solid Lipid Nanoparticles Interactions with a Model of Bio-Membrane: A Comparison with In Vitro Skin Permeation Data. Pharmaceuticals, 11(4), 138.

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Low-Intensity Laser Therapy Evaluation

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A therapeutic low-intensity red (InGaAIP) and near-infrared (GaAlAs) laser (100 mW;
Photon Lase III), with emissions of 660 and 808 nm, was purchased from D.M.C
Equipamentos Ltda. (Brazil). Ethidium bromide, xylene cyanol, bromophenol blue,
glycerol, agarose, tris(hydroxymethyl)aminomethane (tris), and ethylenediamine
tetraacetic acid (EDTA) were from Merck (USA). Sodium chloride (NaCl), acetic acid,
and sodium hydroxide (NaOH) were from Vetec (Brazil). DNA repair enzyme from
E. coli (T₄ endonuclease V) was from New England
Biolabs (USA).

Fonseca A.S., Campos V.M., Magalhães L.A, & Paoli F. (2015). Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers. Brazilian Journal of Medical and Biological Research, 48(10), 929-938.

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Topical Methyl Salicylate Formulation Analysis

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Metsal^™ cream (iNova Pharmaceuticals, Chatswood, Australia) containing 28.3% methyl salicylate was used in the present study. Sodium azide, phosphate buffered saline sachets (pH 7.4), phosphoric acid of analytical grade, methyl salicylate, and salicylic acid, Fast Blue RR salt, α-naphthyl acetate, Mayer’s hematoxylin, ethylene glycol butyl ether, and Dispase II were purchased from Sigma–Aldrich (Castle Hill, Australia). Tris(hydroxymethyl)aminomethane (TRIS) was purchased from Merck (Darmstadt, Germany), acetone was purchased from Chem Supply (Gillman, Australia), methanol and acetonitrile were purchased from VWR Chemicals (Campbellfield, Australia). Tissue Tek Optimum Cutting Temperature (OCT) embedding medium, cryomold^®, was obtained from ProSciTech (Thuringowa, Australia). All solution preparations used Milli-Q water when required. Croda Australia kindly supplied Brij™ O20/Volpo N20.

Telaprolu K.C., Grice J.E., Mohammed Y.H, & Roberts M.S. (2023). Human Skin Drug Metabolism: Relationships between Methyl Salicylate Metabolism and Esterase Activities in IVPT Skin Membranes. Metabolites, 13(8), 934.

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Antioxidant Evaluation Reagent Procurement

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Folin-Ciocalteu reagent, sodium carbonate anhydrous (Na₂CO₃), gallic acid, sodium nitrite solution (NaNO), sodiumhydroxyde (NaOH), aluminum chloride hexahydrate solution (AlCl₃, 6H₂O), iron (III) chloride anhydrous (FeCl₃), and catechin were purchased from Fluka (Buchs, Switzerland). 2,2-diphenyl-1-picrylhydrazyl-(DPPH), 2,6-di-tert-butyl-4-hydroxytoluene (BHT), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonate (ABTS) were purchased from Sigma-Aldrich (Murcia, spain), Tris (hydroxymethyl)-aminomethane (TRIS), and l-cysteine of analytical grade were obtained from Merck, Darmstadt, Germany. 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) all of analytical grade were obtained from Fluka, Stenheim, Germany. Sodium dodecyl sulfate (SDS) was obtained from AppliChem GmbH, Darmstadt, Germany. Commercial mix was purchased from a supermarket (Murcia, spain).

Hajlaoui H., Arraouadi S., Mighri H., Chaaibia M., Gharsallah N., Ros G., Nieto G, & Kadri A. (2019). Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage. Antioxidants, 8(10), 442.

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Purification and Characterization of BaseMuncher Endonuclease

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All commercially available chemicals were
used without further purification. BaseMuncher endonuclease was purchased
from AbCam. Ampicillin, dithiothreitol (DTT), and isopropyl-β-D-1-thiogalactopyranoside (IPTG) were purchased from Formedium. Escherichia coli DH5α (high efficiency) and
BL21(DE3) cells and Gibson Assembly Cloning Kit were purchased from
New England Biolabs. Ethylenediaminetetraacetic acid (EDTA)-free Complete
protease inhibitor cocktail was purchased from Roche. Deuterium oxide
(D₂O), sodium deuteroxide, 2-(N-morpholino)ethanesulfonic
acid (MES), N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic
acid (TAPS), glycerol, imidazole, lysozyme, NiCl₂, tris(hydroxymethyl)aminomethane
(Tris), polyethylene glycol 8000 (PEG-8000), and dUMP were purchased
from Merck. Agarose, dNTPs, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic
acid (HEPES), NaCl, and Phusion high-fidelity polymerase were purchased
from ThermoFisher Scientific. Tobacco etch virus protease (TEVP) was
produced as previously described.^{11 (link)}

Devi S., Carberry A.E., Zickuhr G.M., Dickson A.L., Harrison D.J, & da Silva R.G. (2023). Human 2′-Deoxynucleoside 5′-Phosphate N-Hydrolase 1: Mechanism of 2′-Deoxyuridine 5′-Monophosphate Hydrolysis. Biochemistry, 62(17), 2658-2668.

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Acetylcholine and Choline Quantification Protocol

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Reagents. Acetyl choline chloride, choline chloride, acetylcholinesterase, choline oxidase , diisopropyl phosphorofluoridate, and sesame oil were all purchased from Sigma Chemical Co., Ltd. (St. Louis, MO); potassium phosphate monobasic was purchased from Fisher Scientific Co. (Fair Lawn, NJ); tetramethylammonium chloride, sodium azide, tris(hydroxymethyl) aminomethane (Tris), and ethylenediaminetetraacetic acid, disodium salt, dihydrate (EDTA) were purchased from Aldrich Chemical Co. Inc. (Milwaukee, WI). Bromoethane and 3-dimethylaminol-propanol (Aldrich), were employed to synthesize the internal standard, ethylhomocholine bromide (N,N-dimethyl-N-ethyl-3-amino-1-propanol bromide), Octyl sodium sulphate was purchased from Eastman Kodak Co. (Rochester, NY). All other chemicals used were of analytical grade and of the highest available purity and used without purification.

NWOBI N.L., ADEDAPO S.K., OLUKOLADE O., OYINLADE O.A., LAGUNJU I.A., ATULOMAH N.O., NWAZUOKE I.A, & ANETOR J.I. (2020). Positive and inverse correlation of blood lead level with erythrocyte acetylcholinesterase and intelligence quotient in children: implications for neurotoxicity. Interdisciplinary Toxicology, 12(3), 136-142.

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Multifunctional Nanoparticles for Drug Detection

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Materials: Hexadecyltrimethylammonium chloride (CTAC, 25 wt % in water), hydrogen tetrachloroaurate trihydrate (HAuCl4•H2O, ≥99.9%), crystal violet (CV, ≥90%), L-ascorbic acid (AA, ≥99%), O-[2-(3mercaptopropionylamino)ethyl]-O'-methylpolyethylene glycol (mPEG-SH, average MW: 5.000), sodium borohydride (NaBH4, 99%), tetraethylorthosilicate (TEOS), (3-isocyanatopropyl)triethoxysilane, sodium hydroxide (NaOH), (3-aminopropyl)triethoxysilane (APTES), hydrochloric acid, n-cetyltrimethylammonium bromide (CTAB, ≥99%) and tris(hydroxymethyl)aminomethane (Tris) were purchased from Aldrich. Milli-Q water (resistivity 18.2 MΩ•cm at 25 °C) was used in all experiments. The oligonucleotides and aptamers listed in Table 3 were provided by Isogen Life Science. Analytical-grade solvents were from Scharlab (Barcelona, Spain). Cocaine hydrochloride, morphine, methadone and heroin hydrochloride were provided by the Agencia Española de Medicamentos y Productos Sanitarios (AEMPS). All chemicals were used as received.

Oroval M., Coronado-Puchau M., Langer J., Sanz-Ortiz M.N., Ribes Á., Aznar E., Coll C., Marcos M.D., Sancenón F., Liz-Marzán L.M, & Martínez-Máñez R. (2016). Surface Enhanced Raman Scattering and Gated Materials for Sensing Applications: The Ultrasensitive Detection of Mycoplasma and Cocaine. Chemistry (Weinheim an der Bergstrasse, Germany), 22(38).

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Mild Steel Corrosion Inhibition Protocol

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Tetraethyl
orthosilicate (TEOS, 98%), hexadecyltrimethylammonium bromide (CTAB,
99%), triblock copolymer F127 (Pluronic F127), ammonium hydroxide
(32%), ethanol (99.8%), 1H-benzotriazole, hydrochloride
dopamine, and tris (hydroxymethyl) aminomethane (Tris) were purchased
from Sigma-Aldrich, U.K. The investigated mild steel was supplied
by Metal Store, U.K.

Qian B., Zheng Z., Michailids M., Fleck N., Bilton M., Song Y., Li G, & Shchukin D. (2019). Mussel-Inspired Self-Healing Coatings Based on Polydopamine-Coated Nanocontainers for Corrosion Protection. ACS Applied Materials & Interfaces, 11(10), 10283-10291.

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