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9 protocols using cadmium sulfate

1

Cadmium Sulfide Quantum Dots Synthesis

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SWCNTs (diameter, <8 nm; length, 10–30 μm), cadmium sulfate (CdSO4, 99.99% purity), pyrrole and sodium sulfide (Na2S, 98% purity) were purchased from Sigma-Aldrich (India). Green tea (Lipton) was picked from the Indian market for preparing the CdS QDs. The experimental material used for photocatalytic degradation was Ponceau BS dye (Sigma-Aldrich). Castrol engine oil, class: 15W-40 was used as a base fluid to study the rheology. Double distilled water was used in synthesis. All chemical reagents were of analytical grade.
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2

Inhibition of Ubiquitin-Proteasome System

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TAK243 (Ub-activating E1 enzyme inhibitor, Selleckchem, Houston, TX) and ML792 (SUMO E1 inhibitor, MedKoo Bioscience, Morrisville, NC) were dissolved in DMSO and used at a final concentration of 0.1% DMSO. A 20S proteasome assay kit was purchased from (Enzo, Famingdale, NY). Bismuth (III) nitrate pentahydrate and antimony (III) chloride were purchased from Wako (Osaka, Japan). They were dissolved in 0.1 M citrate buffer (pH 7.4) at 10 mM and used as stock solutions. Sodium m-arsenite was purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in PBS solution. These solutions were sterilized using a syringe filter (0.45 μm pore size). Unless otherwise specified, common chemicals of analytical grade including cadmium sulfate were obtained from Sigma-Aldrich or Wako.
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3

Synthesis of Metal Chalcogenide Nanomaterials

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Nickel sulfate hexahydrate (NiSO4.6H2O), lead(II) nitrate (Pb(NO3)2), cobalt chloride hexahydrate (CoCl2.6H2O), copper sulfate pentahydrate (CuSO4·5H2O), selenium (Se), zinc acetate dehydrate [Zn(CH3COO)2.2H2O], thioacetamide (C2H5NS), urea (CH4N2O), acetic acid (C2H4O2), cadmium acetate dehydrate [Cd(CH3COO)2.2H2O], sulfur (S), potassium chloride (KCl), sodium sulfide (Na2S), cadmium sulfate (CdSO4·8/3H2O), nitro tri-acetic acid (N(CH2CO2H)3), sodium thiosulfate (Na2S2O3), N-methyl-2-pyrrolidone (C5H9NO), polyvinylidene fluoride (PVDF), and Ni-foams were purchased from Sigma-Aldrich and used without further purification. TiO2 paste (Ti-Nanoxide HT/SP) was supplied by Solaronix. The MWCNTs (Carbon Nano-Material Technology Co., Ltd) were used as received.
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4

Graphene-based Electrochemical Sensors

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The reagents used for this study, namely graphene oxide, aniline monomer, potassium persulfate (PPS), 3-aminopropyltriethoxysilane (APTES, 98%), hydrochloric acid, ethanol (EtOH), acetate buffer, lead sulfate, nickel chloride, copper sulfate, cadmium sulfate, manganese sulfate, sodium nitrite, sodium hydrogen phosphate and sodium hydroxide, were supplied by Sigma-Aldrich (St. Louis, MO, USA) and Alfa Aesar (Karlsruhe, Germany). The reagents were used for the study as received. The reagents were prepared using deionized water (18.6 MΩ cm−1).
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5

Synthesis and Characterization of Copper-based Materials

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Copper(I) chloride beads (CuCl, anhydrous,
≥99.99%, Sigma-Aldrich), copper(II) chloride powder (CuCl2, anhydrous, ≥99.995%, Sigma-Aldrich), copper(I) oxide
powder (Cu2O, anhydrous, ≥99.99%, Sigma-Aldrich),
copper(II) oxide powder (CuO, 99.999%, Sigma-Aldrich), ammonium chloride
powder (NH4Cl, anhydrous, ≥99.5%, Sigma-Aldrich),
2-methoxyethanol (2ME, anhydrous, 99.8%, Sigma-Aldrich), ethanolamine
(EA, ≥99.5%, Sigma-Aldrich), thioglycolic acid (TGA, ≥99%,
Sigma-Aldrich), cadmium sulfate (CdSO4, ≥99.99%,
Sigma-Aldrich), ammonia solution (NH3·H2O, 25%, Honeywell), thiourea (≥99.0%, Sigma-Aldrich), potassium
perruthenate (KRuO4, 97%, Alfa Aesar), and hydrophobic
polytetrafluoroethylene (PTFE) filters (0.20 μm pore size, 13
mm diameter, Merck) were used as received.
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6

Synthesis of Colloidal Nanocrystals

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Copper chloride (CuCl2·2H2O, >99.99%),
tin chloride (SnCl2·2H2O, >99.99%),
sulfur powder (S, >99.99%), cadmium
sulfate (CdSO4, >99.99%), zinc sulfate (ZnSO4·7H2O, >99.99%), octadecane (ODE, technical grade,
70%), oleylamine (OLA, technical grade, 70%), isopropyl alcohol (IPA,
technical grade, 70%), toluene (technical grade, 70%), dichloromethane
(DCM, TLC high-purity grade, ≥99.8%), and tetrabutylammonium
perchlorate (TBAP, ≥99.0%) were purchased from Sigma Aldrich.
All chemicals were used as received from suppliers without any further
purification.
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7

Synthesis of Hexanoyl Chitosan

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The chitosan 90/60/A1 (origin crab shell, Mw = 90–150 kDa, manufacturer’s specification) was purchased from Biolog Heppe® GmbH (Landsberg, Germany) and used without further purification. The indexes relate to a deacetylation degree of 90%, a viscosity of 60 mPas, and 1% ash content. Hexanoyl chloride, hydrochloric acid (37%), sodium hydroxide, and cadmium sulfate were purchased from Sigma Aldrich (Taufkirchen, Germany) and used without any further purification. Concentrated acetic acid was purchased from Merck KGaA (Darmstadt, Germany), whilst methanol and acetone were purchased from VWR (99%) (Darmstadt, Germany). As adsorption and reaction media ultrapure water obtained by a Milli-Q Advantage A10® system from Merck KGaA (Darmstadt, Germany), (TOC 5 ppb, resistivity of 18.2 MΩ*cm at 25 °C) was used.
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8

Cytotoxicity Evaluation of KRG-WE

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KRG-WE was obtained from Korean Ginseng Corporation (Daejeon, Korea). Sodium dodecyl sulfate (SDS), 3-(4,5-dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT), Cadmium sulfate and an ERK inhibitor (U0126) were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Biotechnics Research (Lake Forest, CA, USA), and Ham's F–12K (Kaighn's) medium, Roswell Park Memorial Institute 1640 (RPMI 1640) and Dulbecco's Modified Eagle Medium (DMEM) were obtained from HyClone (Grand Island, NY, USA). The lung alveolar epithelial cell line (A549) (No. KCLB-10185) was acquired from the Korean Cell Line Bank (KCLB, Seoul, Korea) and human embryonic kidney cell line (HEK293T) (No. CRL-3216) was purchased from ATCC (Rockville, MD, USA). The AP-1 binding site-containing luciferase construct was used as reported previously [21 (link)]. Antibodies (phospho-specific and total) were obtained from Cell Signaling Technology (Beverly, MA, USA).
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9

Colorimetric Detection Assay Development

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Aluminium potassium sulphate, glacial acetic acid, sulphanilic acid, 1-naphthol, cadmium sulfate, Zn powder, Potassium permanganate, aqueous methanol, Folin-Ciocalteu and gallic acid were obtained from Sigma (Germany). Other chemicals were purchased from Al-Gomhoria company, Egypt.
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