Cover glass

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Cover glass is a thin, transparent glass sheet used as a protective layer in various laboratory applications. Its primary function is to provide a flat, smooth surface for microscopic samples or preparations, allowing for better visibility and analysis during observation under a microscope.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Eclipse e800, by Nikon (2 mentions) F actin, by Thermo Fisher Scientific (2 mentions) Sp8 x inverted confocal microscope, by Leica (2 mentions) Lsm 710 microscope, by Zeiss (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Natural mouse laminin, by Thermo Fisher Scientific (1 mentions) Cyclosporin a, by Bio-Techne (1 mentions) Z 4 hydroxytamoxifen, by Merck Group (1 mentions) Rotenone, by Bio-Techne (1 mentions) Neural tissue dissociation kit p, by Miltenyi Biotec (1 mentions) Bx53 34 fl, by Olympus (1 mentions) Alexa fluor 594 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Laser scanning confocal microscope, by Nikon (1 mentions) Lsm 510 laser scanning system, by Zeiss (1 mentions) Laminin, by Thermo Fisher Scientific (1 mentions) Confocal dishes, by MatTek (1 mentions) Lsm 710, by Zeiss (1 mentions)

Spelling variants of this product

Cover glasses (31 citations) Lab-Tek chambered cover glasses (16 citations) Microscope cover glasses (12 citations) Eight-well chambered cover glasses (8 citations) Chambered cell culture cover glasses (4 citations) 25-mm round microscope cover glasses (3 citations) Optical borosilicate poly‐L‐lysine‐coated sterile glass covers (2 citations) Fisherfinest™ Premium Cover Glasses (2 citations) 45 × 50 mm microscope cover glasses (2 citations) No 1.5 gold seal cover glasses (2 citations)

37 protocols using cover glass

Astrocyte Culture and Ca2+ Imaging

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Primary cortical astrocytes were cultured according to standard protocols, modified from (Schildge et al., 2013 ). Briefly, cortices of P4–7 GLAST-CreER;mGCaMP3/+;IP3R2−/−triple transgenic mouse pups were dissociated using a Neural Tissue Dissociation Kit (P) (Miltenyi Biotec). Isolated cells were plated on a T-75 flask coated with poly-l-lysine (Sigma-Aldrich) and fed with DMEM (Life Technologies) supplemented with 10 % heat-inactivated FBS (Life Technologies) and 1 % penicillin-streptomycin (Life Technologies). After 7 days, astrocytes formed a confluent layer at the bottom of the flask. These cells were then plated on 12 mm cover glass (Thermo Fisher Scientific) coated with poly-l-lysine (Sigma-Aldrich) and Natural Mouse Laminin (Thermo Fisher Scientific) at a density of 20,000 cells per cover glass. One day after plating, expression of mGCaMP3 was induced by applying 1 μM (Z)-4-hydroxytamoxifen (H7904, Sigma-Aldrich). At least 14 days later, astrocyte Ca²⁺ transients were imaged using a Zeiss LSM 710 microscope, as described below. To block mPTP activity, astrocyte cultures were incubated for 1 hour prior to Ca²⁺ imaging in mPTP inhibitors [Cyclosporin A (inhibits cyclophilin D, Tocris, 20 μM; takes long time to dissolve and needs filtration of remaining precipitates) and Rotenone (Mitochondrial complex I inhibitor, Tocris, 10 μM)].

Agarwal A., Wu P.H., Hughes E.G., Fukaya M., Tischfield M.A., Langseth A.J., Wirtz D, & Bergles D.E. (2017). Transient opening of the mitochondrial permeability transition pore induces microdomain calcium transients in astrocyte processes. Neuron, 93(3), 587-605.e7.

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Microscopic Differentiation and Foam Cell Observation

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For microscopic observation, THP-1 cells were differentiated and loaded at a density of 2.5 × 10⁵ cells/well for different time periods on cover glass (Thermo Fisher Scientific) in 24-well cell culture plates. After incubation, adherent cells were washed with phosphate buffered saline (PBS) and then fixed with 3.7% formaldehyde solution. After washing with PBS, differentiated cells were stained with hematoxylin solution, and loaded cells after differentiation were stained with hematoxylin solution followed by working solution of oil red O for assessing the extent of foam cell formation. The plates were washed with distilled water and observed under the microscope (BX53-34-FL, Olympus). A semiquantification of lipid droplets stained by oil red O was performed with ImageJ (National Institutes of Health).

Yano K., Ohkawa R., Sato M., Yoshimoto A., Ichimura N., Kameda T., Kubota T, & Tozuka M. (2016). Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells. Journal of Lipids, 2016, 9891316.

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Immunofluorescence Analysis of Lm Infection

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HepG2 cells were grown on coverglass (Thermo Fisher Scientific, USA), cocultured with Lm, and then washed three times. Cells were then fixed with 1% formaldehyde in PBS at room temperature for 30 min and then permeabilized in 0.1% Triton X‐100 in PBS for 10 min. Cells were incubated at 37°C for 2 hr with anti‐Lm polyclonal antibody(Shanghai Prajna Biology Technique Co., Ltd. China). HepG2 cells were then incubated with Alexa Fluor 594 goat anti‐rabbit IgG (H+L) (Life Technologies, USA). Extensive washing in PBS, the cells were mounted on slides using a DAPI (H‐1200; VectorLab.) mounting medium. Images were obtained under confocal laser scanning microscope (Nikon, Tokyo, Japan).

Chen G., Wu M., Liu W., Xie M., Zhang W., Fan E, & Liu Q. (2018). Reactive oxygen species inhibits Listeria monocytogenes invasion into HepG2 epithelial cells. Food Science & Nutrition, 6(6), 1501-1507.

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Electrosprayed Droplet Characterization

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Electrosprayed droplets collected on a cover glass (22 mm x 22 mm, Thermo Scientific, Pittsburgh, PA) placed underneath the needle at distances from 3 cm up to 9 cm were examined by optical microscope (Olympus BX-51). Individual droplet sizes were determined using ImageJ (NIH) image analysis software. Deposited droplet size histograms were obtained on a total number of around 300 droplets at each condition.

Guo Q., Mather J.P., Yang P., Boden M, & Mather P.T. (2015). Fabrication of Polymeric Coatings with Controlled Microtopographies Using an Electrospraying Technique. PLoS ONE, 10(6), e0129960.

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Uptake and Localization of RNA Dendrimers

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KB cells were grown in cover glass (Thermo Fisher Scientific, Waltham, MA, USA) in 24 well bottom culture dishes in its complete medium overnight. Cells were seeded at a density of 2×10⁵ cells/ml in a volume of 500 μl. On the day of testing, cells were washed with Opti-MEM medium twice, and RNA dendrimer (with whole chain Cy5 labeled SquareE-strand) were suspended in 200 μl of Opti-MEM medium and incubated with cells at 37°C for 1 hr. Cells were washed with PBS twice, fixed with 4 % paraformaldehyde solution (Microscopy Sciences, Hatfield, PA, USA) in PBS at room temperature for 20 min, then washed with PBS and permeabilized with 0.05% Triton-X 100 in PBS at room temperature for 3 min. The cells were then stained with Alexa488 phallodin (Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 20 min, the wells washed with PBS and air dried. The cells were stained with gold antifade (Thermo Fisher Scientific, Waltham, MA, USA) for DAPI staining for confocal microscopy imaging with Zeiss LSM 510 laser scanning system.

Sharma A., Haque F., Pi F., Shlyakhtenko L.S., Evers B.M, & Guo P. (2015). Controllable Self-assembly of RNA Dendrimers. Nanomedicine : nanotechnology, biology, and medicine, 12(3), 835-844.

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Autoreactivity Staining Assay for mAbs

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Autoreactivity staining assays were performed on HEp-2 cells (Zeus Scientific) per the manufacturer recommendations. mAbs were diluted to 50 and 25 μg/mL using SAVe diluent (Zeus Scientific). A total of 20 μL of the appropriate dilution was coated onto cells fixed on the slide and incubated for 30 minutes at room temperature in a humidified chamber. Slides were rinsed in 1× PBS, washed twice in 1× PBS in Coplin jars for 3–5 minutes, and then stained with 20 μL of FITC-conjugated secondary antibody for 30 minutes in a humidified chamber. Then, slides were rinsed in 1× PBS, washed twice in 1× PBS in Coplin jars for 3–5 minutes, and mounted with 15 μL of mounting media per well and a cover glass (Thermo Fisher Scientific). Slides were imaged on a Nikon Eclipse E800 microscope at 20× in the RGB mode for 2 seconds using SPOT 5.0 software (SPOT Imaging). VRC01, 4E10, VRC07-523, and VRC07-G54W were used as control mAbs for staining and given a score of 0, 1, 2, and 3, respectively, based on their staining intensity. Test mAbs were assigned scores based on visual comparisons of staining intensity to the control antibodies.

Kisalu N.K., Pereira L.D., Ernste K., Flores-Garcia Y., Idris A.H., Asokan M., Dillon M., MacDonald S., Shi W., Chen X., Pegu A., Schön A., Zavala F., Balazs A.B., Francica J.R, & Seder R.A. (2021). Enhancing durability of CIS43 monoclonal antibody by Fc mutation or AAV delivery for malaria prevention. JCI Insight, 6(3), e143958.

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Cardiomyocyte Isolation and Culture

Cited 2 times

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On day (7 + 4), mESC-CMs were isolated as previously described [19 (link)–21 (link)]. The isolated mESC-CMs were plated on cover glass (Thermo Fisher Scientific) in a 24-well plate or confocal dishes (MatTek, Ashland, MA, USA) that have been pre-coated with 20 μg/mL laminin (Thermo Fisher Scientific) in 0.1% gelatin in 37 °C, 5% CO₂ incubator.
TRPA1 activator or blocker treatment experiments were performed on day (7 + 5). For experiments involving TRPA1 knockdown or TRPA1 knockdown concomitant with PGC-1α overexpression, mESC-CMs were infected with adenoviruses on day (7 + 5) and the experiments were performed on day (7 + 9). For TRPA1 or PGC-1α overexpression experiments, mESC-CMs were infected with adenoviruses on day (7 + 5) and the experiments were performed on day (7 + 7).

Ding Q., Liu X., Qi Y., Yao X, & Tsang S.Y. (2023). TRPA1 promotes the maturation of embryonic stem cell-derived cardiomyocytes by regulating mitochondrial biogenesis and dynamics. Stem Cell Research & Therapy, 14, 158.

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Autoreactivity Profiling of Monoclonal Antibodies

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Autoreactivity staining assays were performed on HEp-2 cells per
the manufacturer recommendations (Zeus Scientific, Branchburg, NJ). MAbs
were diluted to 50 and 25 μg/ml using SAVe Diluent. 20μl
of the appropriate dilution was coated onto cells fixed on the slide and
incubated for 30 minutes at room temperature in a humidified chamber.
Slides were rinsed in 1X PBS, washed twice in 1X PBS in Coplin jars for
3-5 minutes and then stained with 20μl of FITC-conjugated
secondary antibody for 30 minutes in a humidified chamber. Slides were
rinsed in 1X PBS, washed twice in 1X PBS in Coplin jars for 3-5 minutes
and mounted with 15μl of mounting media per well and a cover
glass (Thermo Scientific). Slides were imaged on a Nikon Eclipse E800
microscope at 20X in the RGB mode for 2 seconds using SPOT 5.0 software
(SPOT Imaging, Sterling Heights, MI). VRC01, 4E10, VRC07-523 and
VRC07-G54W were used as control mAbs for staining and given a score of
0, 1, 2 and 3 respectively based on their staining intensity. Test
antibodies were assigned scores based on visual comparisons of staining
intensity to the control antibodies.

Krebs S.J., Kwon Y.D., Schramm C.A., Law W.H., Donofrio G., Zhou K.H., Gift S., Dussupt V., Georgiev I.S., Schätzle S., McDaniel J.R., Lai Y.T., Sastry M., Zhang B., Jarosinski M., Ransier A., Chenine A.L., Asokan M., Bailer R.T., Bose M., Cagigi A., Cale E.M., Chuang G.Y., Darko S., Driscoll J.I., Druz A., Gorman J., Laboune F., Louder M.K., McKee K., Mendez L., Moody M.A., O’Sullivan A.M., Owen C., Peng D., Rawi R., Sanders-Buell E., Shen C.H., Shiakolas A.R., Stephens T., Tsybovsky Y., Tucker C., Veradi R., Wang K., Zhou J., Zhou T., Georgiou G., Alam S.M., Haynes B.F., Rolland M., Matyas G.R., Polonis V.R., McDermott A., Douek D.C., Shapiro L., Tovanabutra S., Michael N.L., Mascola J.R., Robb M.L., Kwong P.D, & Doria-Rose N.A. (2019). Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual. Immunity, 50(3), 677-691.e13.

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In Situ Proximity Ligation Assay of mESCs

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mESCs were dried on coverglass (Fischer) and fixed in 4% paraformaldehyde for 10 min at room temperature, washed twice with PBS, and permeabilized with 0.1% (vol/vol) Triton X-100 for 30 min. Then the in-situ proximity ligation assay (in situ PLA) was performed according to the manufacturer’s protocol (Duolink #DUO92007, #DUO92001, #DUO92005) Briefly, after the cells were treated with a blocking solution for 1 h at room temperature, primary antibodies (diluted at 1:50) were incubated overnight at 4°C. The probes were incubated for 1 h at 37°C, followed by hybridization, ligation, amplification, and detection. After the cells were washed with Buffer A (3x) and B (2x), samples were counterstained with DAPI. Images were taken at 60X on a DeltaVision Elite Deconvolution microscope.

Ramabadran R., Wang J.H., Reyes J.M., Guzman A.G., Gupta S., Rosas C., Brunetti L., Gundry M.C., Tovy A., Long H., Gu T., Cullen S.M., Tyagi S., Rux D., Kim J.J., Kornblau S.M., Kyba M., Stossi F., Rau R.E., Takahashi K., Westbrook T.F, & Goodell M.A. (2023). DNMT3A-coordinated splicing governs the stem state switch toward differentiation in embryonic and hematopoietic stem cells. Nature cell biology, 25(4), 528-539.

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Autoreactivity Staining Assay Protocol

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Autoreactivity staining assays were performed on HEp-2 cells per the manufacturer recommendations (Zeus Scientific, Branchburg, NJ). MAbs were diluted to 50 and 25 μg/ml using SAVe Diluent. 20μl of the appropriate dilution was coated onto cells fixed on the slide and incubated for 30 minutes at room temperature in a humidified chamber. Slides were rinsed in 1× PBS, washed twice in 1× PBS in Coplin jars for 3–5 minutes and then stained with 20μl of FITC-conjugated secondary antibody for 30 minutes in a humidified chamber. Slides were rinsed in 1× PBS, washed twice in 1× PBS in Coplin jars for 3–5 minutes and mounted with 15μl of mounting media per well and a cover glass (Thermo Scientific). Slides were imaged on a Nikon Eclipse E800 microscope at 20× in the RGB mode for 2 seconds using SPOT 5.0 software (SPOT Imaging, Sterling Heights, MI). VRC01, 4E10, VRC07-523 and VRC07-G54W were used as control mAbs for staining and given a score of 0, 1, 2 and 3 respectively based on their staining intensity. Test antibodies were assigned scores based on visual comparisons of staining intensity to the control antibodies.

Cale E.M., Gorman J., Radakovich N.A., Crooks E.T., Osawa K., Tong T., Li J., Nagarajan R., Ozorowski G., Ambrozak D.R., Asokan M., Bailer R.T., Bennici A.K., Chen X., Doria-Rose N.A., Druz A., Feng Y., Joyce M.G., Louder M.K., O’Dell S., Oliver C., Pancera M., Connors M., Hope T.J., Kepler T.B., Wyatt R.T., Ward A.B., Georgiev I.S., Kwong P.D., Mascola J.R, & Binley J.M. (2017). Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop. Immunity, 46(5), 777-791.e10.

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