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4 protocols using rhodamine b

1

Synthesis and Characterization of Fluorescent Dyes

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Urea (CH4N2O, 99.5%), and thioUrea (CH4N2S, 99%) were bought from Merck, Mumbai, India. Rhodamine B (99.9%) was obtained from Loba Chemie Pvt. Ltd., Mumbai, India. All reagents were used without further purification as received.
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2

Synthesis of Plasmonic Nanoparticles

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Copper sulfate pentahydrate (CuSO4·5H2O, 99%), polyethylene glycol (PEG), sodium
hydroxide (NaOH, 98%),
hydrochloric acid, silver nitrate (AgNO3, 99%), trisodium
citrate (99%), 4-nitrophenol, rhodamine B, and indigo carmine (98%)
are procured from Loba Chemie Pvt, Ltd. Ascorbic acid (99%) and sodium
borohydride (NaBH4, 98%) are purchased from Sigma-Aldrich.
Tetrachloroauric acid (HAuCl4,) is procured from Spectrochem
Pvt. Ltd. All of the chemicals are used without any further purification.
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3

Phytochemical Analysis of P. guajava Leaves

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Fresh green leaves of P. guajava were taken from an orchid in Fatehgarh Sahib (Punjab) India. Tetracycline (Sigma Aldrich), ferrous sulphate, copper sulphate, methylene blue, rhodamine B were purchased from LOBA Chemie Pvt Ltd. All the reagents were of analytical grade. Deionized (DI) water was employed for preparing different solutions.
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4

Carvedilol Nanoparticle Formulation Development

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Carvedilol was obtained as a gift sample from Global Napi Pharmaceutical Company (Egypt); glyceryl behenate (Compritol 888 ATO) and glyceryl palmitostearate (Precirol ATO 5) were kindly donated by Gattefossé (France); L-α- phosphatidylcholine (soybean lecithin), poloxamer 407, poloxamer 188, polyacrylic acid (Carbopol 971P), formic acid (HPLC grade), acetonitrile (HPLC grade), disodium hydrogen phosphate, and dialysis bags with a molecular weight cutoff of 12,000 g/mol were purchased from Sigma-Aldrich (USA); and rhodamine B was purchased from Loba Chemie (India). All other ingredients used were of analytical grade.
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