Bbl campy pouch system

Manufactured by BD

Sourced in United States

The BBL Campy Pouch® System is a laboratory equipment product designed for the isolation and identification of Campylobacter species. It provides a controlled atmosphere for the growth and detection of Campylobacter bacteria.

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Lab products found in correlation

Chocolate agar plates, by BD (10 mentions) Atcc crl 1739, by American Type Culture Collection (3 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Columbia agar plate, by bioMérieux (1 mentions) Fetal calf serum (fcs), by Merck Group (1 mentions) H pylori selective supplement, by Thermo Fisher Scientific (1 mentions) Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

13 protocols using bbl campy pouch system

H. pylori Infection in AGS Cells

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The human gastric epithelial AGS cells (gastric adenocarcinoma, ATCC CRL 1739; American Type Culture Collection, Rockville, MD, USA) were grown as described previously [18 (link)]. H. pylori cells (NCTC 11637, American Type Culture Collection) were grown on chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) at 37°C, under microaerophilic conditions, using an anaerobic chamber (BBL Campy Pouch^® System; Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA). The H. pylori strain NCTC 11637 expresses the proteins of CagA, VacA, cytotoxin and urease [19 (link)].
AGS cells were seeded overnight to reach 80% confluency. The H. pylori was harvested and suspended in antibiotic-free RPMI 1640 medium supplemented with 10% FBS, and then added to the AGS cell culture at a 50 : 1 ratio of bacterium : AGS cells. AGS cells (1.0 × 10⁵/mL) were pre-treated with 5 or 10 μM β-carotene (dissolved in tetrahydrofuran [17 ]) for 2 hours prior to the addition of the H. pylori. The infected cells were incubated for an additional 6 hours for determination of the levels of p-GSK3β, GSK3β, and β-catenin or 24 hours for determination of cell viability and the levels of c-myc and cyclin E) in the presence of H. pylori. The 5 and 10 μM β-carotene concentrations used in the pre-treatment are reportedly nontoxic [20 (link),21 (link)].

Kim D., Lim J.W, & Kim H. (2019). β-carotene Inhibits Expression of c-Myc and Cyclin E in Helicobacter pylori-infected Gastric Epithelial Cells. Journal of Cancer Prevention, 24(3), 192-196.

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Helicobacter pylori Strain Cultivation

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H. pylori strain NCTC 11637 was obtained from ATCC, and the bacterial cells were grown on chocolate agar plates (Becton-Dickinson Microbiology Systems, Franklin Lakes, NJ, USA) at 37 °C under microaerophilic conditions using an anaerobic chamber (BBL Campy Pouch^® System, Becton-Dickinson Microbiology Systems). H. pylori was incubated overnight in a humidified CO₂ incubator before inoculation. Subsequently, H. pylori was collected from the plates, suspended in antibiotic-free RPMI 1640 medium supplemented with 10% FBS, and added to the cell culture at a cell/bacterium ratio of 1:100.

Kim S.H, & Kim H. (2021). Inhibitory Effect of Astaxanthin on Gene Expression Changes in Helicobacter pylori-Infected Human Gastric Epithelial Cells. Nutrients, 13(12), 4281.

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Cultivation of Virulent H. pylori Strain

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H. pylori, strain NCTC 11637, was obtained from the American Type Culture Collection (Rockville, MD). The genotype of this bacterium is cagA⁺ and vacA⁺ and was inoculated on chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) at 37°C under microaerophilic conditions using an anaerobic chamber (BBL Campy Pouch System, Becton Dickinson Microbiology Systems) [28 ].

Byun E., Lim J.W., Kim J.M, & Kim H. (2014). α-Lipoic Acid Inhibits Helicobacter pylori-Induced Oncogene Expression and Hyperproliferation by Suppressing the Activation of NADPH Oxidase in Gastric Epithelial Cells. Mediators of Inflammation, 2014, 380830.

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Cytotoxic H. pylori Infection Assay

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Experiments were performed with the cytotoxic (CagA⁺/VacA⁺) reference strain of H. pylori 26695 (ATCC). H. pylori was grown under microaerophilic conditions on Columbia agar plates (bioMérieux, Marcy-l'Etoile, France) containing 100 U/ml H. pylori selective supplement (Oxoid, Basingstoke, UK) at 37°C in an anaerobic chamber (BBL Campy Pouch System; Becton Dickinson Microbiology Systems, San Diego, CA) for 48-72 h. The cells were harvested and resuspended in antibiotic-free RPMI-1640 medium (Invitrogen) supplemented with 2% fetal calf serum (Sigma-Aldrich). The bacterial densities were adjusted by optical density (OD) measurements at 660 nm, in which 1 OD₆₆₀ = 1×10⁸ colony-forming units (CFU)/ml. H. pylori was then incubated with GES-1, AGS, or SGC7901 cells at a bacteria/cell ratio of 100:1 in culture medium for the required times.

Zhu R., Gao C., Wang L., Zhang G., Zhang W., Zhang Z., Shen L, & Wang S. (2018). Involvement of Aryl Hydrocarbon Receptor and Aryl Hydrocarbon Receptor Repressor in Helicobacter Pylori-related Gastric Pathogenesis. Journal of Cancer, 9(15), 2757-2764.

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Infecting AGS Cells with H. pylori

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The H. pylori strain NCTC 11637 was obtained from the American Type Culture Collection. The bacterial cells were grown on chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysvile, MD, USA) at 37 °C, under microaerophilic conditions, using an anaerobic chamber (BBL Campy Pouch^® System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA). AGS cells were cultured and seeded overnight to reach 80% confluency. The H. pylori was harvested from chocolate agar plates, suspended in antibiotic-free RPMI 1640 medium supplemented with 10% fetal bovine serum, and then added to the AGS cell culture at a cellular ratio of 50:1.

Kim S.H., Lim J.W, & Kim H. (2018). Astaxanthin Inhibits Mitochondrial Dysfunction and Interleukin-8 Expression in Helicobacter pylori-Infected Gastric Epithelial Cells. Nutrients, 10(9), 1320.

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Co-culture of H. pylori and AGS Cells

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H. pylori, strain NCTC 11637, was obtained from the American Type Culture Collection. The bacterium was grown under microaerophilic conditions at 37 °C, using an anaerobic chamber (BBL Campy Pouch System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA).
AGS cells were seeded and cultured overnight to reach 80% confluency. Prior to H. pylori infection, the cells were washed once with culture medium containing no antibiotics. Whole H. pylori was suspended in antibiotic-free RPMI 1640 medium supplemented with 10% fetal bovine serum, and then treated to the AGS cells. The ratio of AGS cell: H. pylori was 1:100. The ratio of AGS cell: H. pylori, 1:100, was adapted from our previous study showing high expression of IL-8 in gastric epithelial AGS cells infected with H. pylori, NCTC11637 [30 (link)].

Kyung S., Lim J.W, & Kim H. (2019). α-Lipoic Acid Inhibits IL-8 Expression by Activating Nrf2 Signaling in Helicobacter pylori-infected Gastric Epithelial Cells. Nutrients, 11(10), 2524.

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Culturing Helicobacter pylori on Chocolate Agar

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All experiments were performed with H. pylori, cag A positive strain 60190 (ATCC 49503). Chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysvile, MD, USA) were used to grow the bacteria at 37 °C under microaerophilic conditions using an anaerobic chamber (BBL Campy Pouch^® System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA).

Bae S., Lim J.W, & Kim H. (2021). β-Carotene Inhibits Expression of Matrix Metalloproteinase-10 and Invasion in Helicobacter pylori-Infected Gastric Epithelial Cells. Molecules, 26(6), 1567.

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H. pylori Infection of AGS Cells

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H. pylori, strain NCTC (National Collection of Type Cultures) 11637 (cag A-positive, vac A-positive strain), was obtained from the American Type Culture Collection. The bacterium was inoculated onto chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysvile, MD, USA) at 37 °C under microaerophilic conditions using an anaerobic chamber (BBL Campy Pouch System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA). AGS cells were cultured overnight to reach 80% confluency. Whole H. pylori was harvested from the chocolate agar plates, suspended in antibiotic-free RPMI 1640 medium supplemented with 10% fetal bovine serum, and then used to treat AGS cells. AGS cells were cultured in the presence of H. pylori at a cell to a H. pylori ratio of 1:50 (at a multiplicity of infection (MOI) of 50).

Lee H., Lim J.W, & Kim H. (2020). Effect of Astaxanthin on Activation of Autophagy and Inhibition of Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cell Line AGS. Nutrients, 12(6), 1750.

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H. pylori Infection of AGS Cells

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The H. pylori NCTC 11637 used in this study was a cagA- and vacA-positive standard strain [24 (link)]. It was obtained from the American Type Culture Collection and inoculated on chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) in an anaerobic chamber (BBL Campy Pouch System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA) at 37 °C under microaerophilic conditions.
AGS cells were seeded and cultured overnight to reach 80% confluency. Prior to H. pylori infection, the cells were washed with antibiotic-free culture medium. H. pylori cells were harvested from the chocolate agar plates, suspended in antibiotic-free RPMI 1640 medium supplemented with 10% fetal bovine serum, and then added to the AGS cells.

Park Y., Lee H., Lim J.W, & Kim H. (2019). Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells. Antioxidants, 8(12), 637.

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Helicobacter pylori Infection Model in AGS Cells

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The H. pylori strain NCTC 11637 was obtained from ATCC. The bacterial cells were grown on chocolate agar plates (Becton Dickinson Microbiology Systems, Cockeysvile, MD, USA) at 37 °C, under microaerophilic conditions, using an anaerobic chamber (BBL Campy Pouch^® System, Becton Dickinson Microbiology Systems, Franklin Lakes, NJ, USA). AGS cells were seeded and cultured overnight to reach 80% confluency. The H. pylori was harvested from the plates, suspended in antibiotic-free RPMI 1640 medium supplemented with 10% fetal bovine serum, and then added to the AGS cell culture at a cellular ratio of 50:1. AGS cells (1.5 × 10⁵/mL) were pretreated with 5 μM ASTX or the control vehicle DMSO for 3 h prior to H. pylori stimulation. The cells were then incubated with H. pylori for 1 h (for preparation of RNA extracts and for determination of mRNA gene expressions).

Kim S.H, & Kim H. (2020). Transcriptome Analysis of the Inhibitory Effect of Astaxanthin on Helicobacter pylori-Induced Gastric Carcinoma Cell Motility. Marine Drugs, 18(7), 365.

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