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Atcc tib 39

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ATCC® TIB-39™ is a cell line derived from the Jurkat clone E6-1 human T cell line. It is a T lymphocyte cell line isolated from the peripheral blood of a patient with acute T cell leukemia.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Heat inactivated calf serum, by Merck Group (1 mentions) Crl 2539, by American Type Culture Collection (1 mentions) Crl 2638, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

TIB-39 (11 citations) EL4 (ATCC® TIB-39™) cells (2 citations)

3 protocols using atcc tib 39

1

Culturing Murine EL4 Lymphoma Cells

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EL4 (ATCC® TIB-39™), derived from lymphoma in C57BL/6 N mouse, was purchased from ATCC. EL4 cells were cultured in DMEM (GIBCO Invitrogen) supplemented with 50 IU/mL penicillin, 50 mg/mL streptomycin (GIBCO Invitrogen) and 10% heat-inactivated calf serum (Sigma, USA). Cultures were maintained by replacement of fresh medium every 3 days, and cell density was kept between 1 X 105 and 1 X 106 cells/mL.
Wang W., Ren G., Hong N, & Jin W. (2019). Exploring the changing landscape of cell-to-cell variation after CTCF knockdown via single cell RNA-seq. BMC Genomics, 20, 1015.
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2

In Vitro Cytotoxicity Assay for Murine Cancer Cell Lines

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The murine EL4 T cell lymphoma (ATCC TIB-39), 4T1 mammary carcinoma (ATCC CRL-2539), and CT26 colon carcinoma (ATCC CRL-2638) cell lines were purchased from ATCC (USA) and maintained as recommended by the provider. For in vitro cytotoxicity assays, the cells were harvested, washed, and dispensed in to 96 well flat bottom plates (Thermo Fisher Scientific) at 5,000/well (EL4, CT26), or 2,500/well (4T1). Next, the cells were cultivated with serial dilutions of the star DOX conjugates C1–C4 for 3 days and their metabolic activity or proliferation was determined by MTT (EL4 cell line), or 3H-Thymidine incorporation assay (4T1, CT26). The inhibition of cell growth was calculated as the IC50, the concentration of cytotoxic drug which inhibited metabolic activity or proliferation by 50 %. At least four parallel samples were used for each experimental condition.
Kostka L., Kotrchová L., Šubr V., Libánská A., Ferreira C.A., Malátová I., Lee H.J., Barnhart T.E., Engle J.W., Cai W., Šírová M, & Etrych T. (2019). HPMA-based Star Polymer Biomaterials with Tuneable Structure and Biodegradability Tailored for Advanced Drug Delivery to Solid Tumours. Biomaterials, 235, 119728.
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3

Establishment of Murine Organospecific Microvascular ECs

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Murine brain (MBrMEC) and bone marrow (MBMMEC)‐derived organospecific microvascular endothelial cells (ECs) were established as described34, 36, 37, 38 and patented (N1170 3915.6, N13/521715). EL4 (ATCC® TIB‐39™) and EL4‐IL2 (ATCC® TIB‐181™) were purchased from ATCC. ECs were cultured in OptiMEM medium supplemented with 2% FBS on Primaria tissue culture‐treated plastic vials (Corning™, VWR). ECs were cultured in OptiMEM supplemented with 2% FBS, and lymphoblasts were maintained in DMEM supplemented with 10% FBS. Upon 80% confluency, ECs were detached with Accutase and used for experiments. All cell culture reagents were purchased from Invitrogen unless otherwise specified.
El Hafny‐Rahbi B., Brodaczewska K., Collet G., Majewska A., Klimkiewicz K., Delalande A., Grillon C, & Kieda C. (2021). Tumour angiogenesis normalized by myo‐inositol trispyrophosphate alleviates hypoxia in the microenvironment and promotes antitumor immune response. Journal of Cellular and Molecular Medicine, 25(7), 3284-3299.
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