Bms309403

Adult pregnant Wistar mice (n = 60; body weight [b.w.] 200–250 g) were purchased from Hunan SJA Laboratory Animal (Hunan, China). All procedures for animal study were approved by The First Affiliated Hospital of Harbin Medical University. PE-like mice model was generated through the intraperitoneal injection of L-NAME (N5751, Sigma-Aldrich, 125 mg/kg b.w.). The pregnant mice were randomly divided into three groups: control (n = 10), L-NAME+vehicle (n = 10), and L-NAME+BMS309403 groups (n = 10). BMS309403 (5258, R&D, 1 mg/kg b.w.) was administered intraperitoneally. Saline was used as the vehicle control of BMS309403. For neutralization experiments, mice were randomly divided into three groups: control (n = 10), L-NAME+normal immunoglobulin G (IgG; n = 10), and L-NAME+IL-17A Ab groups (n = 10). Anti-IL-17A antibody (16-7178-81, eBioscience) was injected intraperitoneally at dose. On the gestational day 18.5 (GD 18.5), the mice were sacrificed. Blood and placenta samples were collected and subjected to subsequent analysis. Blood pressure and urine protein level were detected as previously described.³⁹ (link)