U87MG (ATCC line), LN229, U251, and A172 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS). GBM patient-derived neurospheres and GSC were isolated and cultured as previously described as previously described56 –59 . For primary glia isolation, the cerebral cortex from new born mice (2 or 3 days-old) was collected, homogenized and harvested with Leibovitz’s L-15 medium. After washing with PBS, homogenates were treated with DNase and Trypsin. Cells were cultured in Minimum Essential Medium (MEM/EBSS, GE Healthcare) containing 10% horse serum (HS). Medium was changed every three days. Human astrocyte (ScienCell Research Laboratories, #1800) were maintained in Astrocyte medium (#1801), which enriches growth factors enabling high proliferation, and habituated in MEM/EBSS/10%HS for two weeks before the assay.
Mem ebss
Manufactured by GE Healthcare
MEM/EBSS is a cell culture medium used to support the growth and maintenance of a variety of cell types in vitro. It provides the necessary nutrients, salts, and buffers for cell survival and proliferation. The core function of MEM/EBSS is to establish a balanced environment for cell culture applications.
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Lab products found in correlation
4 protocols using mem ebss
1
Isolation and Culture of Glioma Cell Lines
2
Isolation and Culture of Glioma Cell Lines
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U87MG (ATCC line), LN229, U251, and A172 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS). GBM patient-derived neurospheres and GSC were isolated and cultured as previously described as previously described56 –59 . For primary glia isolation, the cerebral cortex from new born mice (2 or 3 days-old) was collected, homogenized and harvested with Leibovitz’s L-15 medium. After washing with PBS, homogenates were treated with DNase and Trypsin. Cells were cultured in Minimum Essential Medium (MEM/EBSS, GE Healthcare) containing 10% horse serum (HS). Medium was changed every three days. Human astrocyte (ScienCell Research Laboratories, #1800) were maintained in Astrocyte medium (#1801), which enriches growth factors enabling high proliferation, and habituated in MEM/EBSS/10%HS for two weeks before the assay.
3
Culturing Human Colon Cancer Cell Lines
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The human colon cancer cell lines HT-29, Caco-2 and HCT-116 were obtained from the State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (cell line not authenticated). HT-29, Caco-2 and HCT-116 cells were maintained in Dulbecco's modified Eagle's medium: Nutrient mixture F-12 media (DMEM/F-12; HyClone; GE Healthcare Life Sciences), minimum essential medium/Earle's balanced salt solution (MEM/EBSS; HyClone; GE Healthcare Life Sciences) and Iscove's modified Dulbecco's medium (IMDM; HyClone; GE Healthcare Life Sciences), respectively. They were supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences) and 1% penicillin/streptomycin (HyClone; GE Healthcare Life Sciences) at 37°C in a humidified incubator containing 5% CO2. Caco-2 cells were co-treated with 1% non-essential amino acids (HyClone; GE Healthcare Life Sciences).
4
Isolation of Exosome-like Vesicles from NPTr Cells
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NPTr cells95 (link) negatively tested for mycoplasma, purchased from Instituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy, were grown in Minimum Essential Medium with Earle’s Balanced Salts (MEM/EBSS) and 2 mM L-Glutamine (GE Healthcare) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco) and 1% penicillin streptomycin (GE Healthcare). Cells were cultured at with 5% CO2 in a humid atmosphere in T75 flasks and 6-well tissue culture plates. Sub-passages were made when cells reached 70-80% confluence. To isolate exosome-like vesicles, the culture medium was removed, confluent cells were washed with phosphate-buffered saline (PBS) and kept in medium without FBS and antibiotics (exosome depleted) for 24 h.
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