Orca charge coupled device camera

Manufactured by Hamamatsu Photonics

Sourced in Japan

The ORCA charge-coupled device (CCD) camera is a high-performance, scientific-grade camera designed for low-light imaging applications. It features a large-format CCD sensor with high quantum efficiency, low read noise, and high dynamic range. The camera is capable of capturing high-quality, high-resolution images and is often used in fields such as biology, physics, and astronomy.

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Lab products found in correlation

Dmire2 microscope, by Leica (2 mentions) Anti rabbit cy3, by Merck Group (2 mentions) Mouse anti ha epitope igg, by Santa Cruz Biotechnology (2 mentions) Anti mouse oregon green, by Thermo Fisher Scientific (2 mentions) Metamorph software, by Universal Imaging (2 mentions) Spinning disk confocal unit, by Hamamatsu Photonics (1 mentions) Bx60 microscope, by Olympus (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Eclipse ti u inverted fluorescence microscope, by Nikon (1 mentions) Py397 fak, by Thermo Fisher Scientific (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Goat serum, by Merck Group (1 mentions) Vimentin, by Merck Group (1 mentions) A5228, by Merck Group (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Ab40794, by Abcam (1 mentions) Ab32084, by Abcam (1 mentions) Ab51032, by Abcam (1 mentions) Snail, by Cell Signaling Technology (1 mentions)

7 protocols using orca charge coupled device camera

Immunofluorescence Localization of HA-SLC26A3

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Fixed cells transfected with WT or mutant HA-SLC26A3 were blocked for 45 min with 7% normal horse serum, then incubated overnight at 4°C with monoclonal anti-HA (1:300, Cell Signaling Technologies), washed three times in PBS, then incubated 1 h at room temperature with Alexa 546-coupled goat anti-rabbit Ig (Life Technologies). After three final washes, coverslips were inverted and mounted on glass slides using VECTASHIELD mounting medium (Vector Labs, Burlingame, CA). Other antigens were detected with compatible, species-specific fluorophor-coupled secondary Ig. After fixation and immunostaining of filter-grown cells as described above, the filters were excised from their frames and mounted on glass slides under coverslips with VECTASHIELD. Cells were imaged with the Zeiss LSM510 confocal fluorescence microscope or with the Nikon TE2000 inverted microscope interfaced to a PerkinElmer spinning disk confocal unit and an Orca charge-coupled device camera (Hamamatsu, Bridgewater, NJ). Image processing was with Zeiss software or (for images captured by Nikon-PerkinElmer microscope) with Slidebook (Intelligent Imaging Innovations, Denver, CO).

Reimold F.R., Balasubramanian S., Doroquez D.B., Shmukler B.E., Zsengeller Z.K., Saslowsky D., Thiagarajah J.R., Stillman I.E., Lencer W.I., Wu B.L., Villalpando-Carrion S, & Alper S.L. (2015). Congenital chloride-losing diarrhea in a Mexican child with the novel homozygous SLC26A3 mutation G393W. Frontiers in Physiology, 6, 179.

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Visualizing E. coli-HMV Interactions

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HEK293 cells were grown in cell culture and HMVs were prepared as described in Methods. E. coli cells expressing YadA (YadA⁺) and control cells (no YadA, YadA⁻) were grown as described above. Then 500 μL of cells was harvested, washed, and resuspended in 1× PBS (pH 7.4). E. coli cells and HMVs were mixed in a 1:1 ratio in 1× PBS (pH 7.4) and 0.4% BSA and then incubated at 37 °C for ∼1.5 h before imaging. All controls were treated in the same manner. Fluorescence was observed using an Axioplan II upright epifluorescence microscope (Carl Zeiss), a 100× 1.4 NA PlanApo objective. Bright-field and fluorescence images were captured using an ORCA charge‐coupled device camera (Hamamatsu) and iVision software (Biovision Technologies).

Yadavalli S.S., Xiao Q., Sherman S.E., Hasley W.D., Klein M.L., Goulian M, & Percec V. (2018). Bioactive cell-like hybrids from dendrimersomes with a human cell membrane and its components. Proceedings of the National Academy of Sciences of the United States of America, 116(3), 744-752.

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Immunofluorescence Analysis of Cellular Components

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Immunofluorescence analysis (IFA) was as described 64 (link). Antibodies for IFA were used at the following
dilutions: mouse anti-HA-epitope IgG (Santa Cruz Biotechnology Inc.) at 1:1000,
rabbit anti-Rab11 at 1:200 44 (link), rabbit
anti-Rab5a at 1:200 68 (link), mouse anti-p67 at
1:1000 43 (link), mouse anti-BiP at 1:10 000
41 (link), rabbit anti-VSG221 at 1:1000
68 (link), rabbit anti-RabX2 at 1:50 71 (link), rabbit anti-IGP48 (this study) at 1:50.
Secondary antibodies were used at the following dilutions: anti-mouse Oregon
Green (Molecular Probes) at 1:1000 and anti-rabbit Cy3 (Sigma) at 1:1000. Cells
were examined on a Nikon Eclipse E600 epifluorescence microscope fitted with
optically matched filter blocks and a Hamamatsu ORCA charge-coupled-device
camera. Digital images were captured using Metamorph software (Universal Imaging
Corp.) on a computer running the Windows XP operating system (Microsoft Inc.)
and the raw images processed using Photoshop CS6 software (Adobe Systems Inc.).
Confocal z-sections were acquired using a Leica DMIRE2 microscope and
deconvolved using Huygens Professional software.

Allison H., O’Reilly A.J., Sternberg J, & Field M.C. (2014). An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. Microbial Cell, 1(10), 325-345.

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Immunofluorescence Analysis of Cellular Compartments

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Immunofluorescence analysis (IFA) was as described (43 (link)). Antibodies for IFA were used at the following dilutions: mouse anti-HA-epitope IgG (Santa Cruz Biotechnology Inc.) at 1:1000, rabbit anti-Rab11 at 1:200 (54 (link)), rabbit anti-Rab5a at 1:200 (55 (link)), mouse anti-p67 at 1:1000 (56 (link)), mouse anti-BiP at 1:10 000 (57 (link)), rabbit anti-VSG221 at 1:1000 (55 (link)), rabbit anti-RabX2 at 1:50 (58 (link)), rabbit anti-IGP48 (this study) at 1:50. Secondary antibodies were used at the following dilutions: anti-mouse Oregon Green (Molecular Probes) at 1:1000 and anti-rabbit Cy3 (Sigma) at 1:1000. Cells were examined on a Nikon Eclipse E600 epifluorescence microscope fitted with optically matched filter blocks and a Hamamatsu ORCA charge-coupled-device camera. Digital images were captured using Metamorph software (Universal Imaging Corp.) on a computer running the Windows XP operating system (Microsoft Inc.) and the raw images processed using Photoshop CS6 software (Adobe Systems Inc.). Confocal z-sections were acquired using a Leica DMIRE2 microscope and deconvolved using Huygens Professional software.

Allison H., O’Reilly A.J., Sternberg J, & Field M.C. (2014). An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. Microbial Cell, 1(10), 325-345.

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DAPI Staining of Transformed Yeast Cells

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SM12 strains transformed with pJPS10, pSP64 or pNT13 plasmids were grown overnight in SC-ura at 30°C, reinoculated into fresh media and grown to an A₆₀₀ of 0.8–1 in SC-ura at 30°C. Cells were then incubated in 5µg/ml 4',6-diamidino-2-phenylindole (DAPI) for 30 minutes, either untreated or treated with 1mM H₂O₂ for 20 min, washed and resuspended in sterile water and visualized for GFP fluorescence, DAPI staining and Nomarski optics using an Olympus (Tokyo, Japan) BX-60 microscope with a 100× oil immersion objective. Images were captured using a Hamamatsu (Shizuoka, Japan) ORCA charge-coupled device camera.

Paul S., Doering T.L, & Moye-Rowley W.S. (2014). Cryptococcus neoformans Yap1 is required for normal fluconazole and oxidative stress resistance. Fungal genetics and biology : FG & B, 74, 1-9.

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Immunofluorescence Characterization of Epithelial-Mesenchymal Transition

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Samples were fixed with 4% paraformaldehyde for 15 min, washed with PBS, and then permeabilized with 0.3% Triton X-100 for 30 min. After blocking for 1 h with 5% goat serum (Sigma) and 2% bovine serum albumin, the samples were incubated with primary antibody against E-cadherin (3195, 1:300; Cell Signaling), Snail (3895, 1:300; Cell Signaling), αSMA (A5228, 1:200; Sigma), ZO1 (40-2200, 1:300; Thermo Fisher Scientific), vimentin (V2258, 1:200; Sigma), cleaved caspase-3 (9961, 1:200; Cell Signaling), FAK (ab40794, 1:200; Abcam), pY397-FAK (44-625G, 1:50; Invitrogen), paxillin (ab32084, 1:200; Abcam), pY118-paxillin (44-722G, 1:200; Invitrogen), α-catenin (ab51032, 1:200; Abcam), or β-catenin (D10A8, 1:100; Cell Signaling). Samples were then washed with PBS and incubated with Alexa Fluor–conjugated secondary antibodies (1:200; Invitrogen). Nuclei were counterstained with Hoechst 33342 (1:1000; Invitrogen). After additional washes with PBS, samples were visualized using a 20×/0.45 NA air objective on a Nikon Eclipse Ti-U inverted fluorescence microscope (Nikon) equipped with a Hamamatsu ORCA charge-coupled device camera. Image analysis was performed on at least 200 cells for each experimental group over three independent experiments using ImageJ.

Kilinc A.N., Han S., Barrett L.A., Anandasivam N, & Nelson C.M. (2021). Integrin-linked kinase tunes cell–cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1. Molecular Biology of the Cell, 32(5), 402-412.

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Visualizing Fluorescent Proteins in Nematodes

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GFP-and mRFP-tagged fluorescent proteins were visualized in nematodes by mounting on 2% agarose pads with 10 mM tetramisole. Fluorescent images of odIs76 and odIs77 transgenic animals were observed using an AxioImager M1m (Carl Zeiss, Thornwood, NY). A 5 × (numerical aperture 0.15) PlanApo objective was used to detect GFP and mRFP signal.
Imaging was done with an ORCA charge-coupled device camera (Hamamatsu, Bridgewater, NJ) by using iVision software (Biovision Technologies, Uwchlan, PA). Exposure times were chosen to capture at least 95% of the dynamic range of fluorescent intensity of all samples.
GFP fluorescence was quantified by obtaining outlines of worms using images of the mRFP control. The mean fluorescence intensity within each outline was calculated (after subtracting away background coverslip fluorescence) for Ub G76V -GFP and mRFP signals using ImageJ.
Fluorescent images of cyp-34A4::gfp transgenic animals were observed using a Chroma/89 North CrestOptics X-Light V2 spinning disk, a Chroma/89North Laser Diode Illuminator (405 nm and 470 nm lines to detect intestinal autofluorescence and GFP, respectively), and a Photometrics PRIME95BRM16C CMOS camera. Images were collected and analyzed with MetaMorph software.

Joshi K.K., Matlack T.L., Pyonteck S.M., Menzel R., & Rongo C. (2020). Biogenic Amine Neurotransmitters Promote Eicosanoid Production And Protein Homeostasis.

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