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Desktop micro computer tomograph ct 40

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4 protocols using desktop micro computer tomograph ct 40

1

Optimizing Bone Structure Analysis Using µCT

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µCT imaging was performed using the cone-beam Desktop Micro Computer Tomograph “µCT 40” by SCANCO Medical AG, Bruettisellen, Switzerland. The settings were optimized for calcified tissue visualization at 55 kVp with a current of 145 µA and 200 ms integration time for 500 projections/180°. For the segmentation of 3D-volumes, an isotropic voxel size of 8.4 µm and an evaluation script with adjusted grayscale thresholds of the operating system “Open VMS” by SCANCO Medical was used. Volume of interest tibia: The analysis of the bone structure was performed in the proximal metaphysis of the tibia, starting 0.43 mm from an anatomic landmark in the growth plate and extending 1.720 mm (200 tomograms) distally.
Lucas S., Omata Y., Hofmann J., Böttcher M., Iljazovic A., Sarter K., Albrecht O., Schulz O., Krishnacoumar B., Krönke G., Herrmann M., Mougiakakos D., Strowig T., Schett G, & Zaiss M.M. (2018). Short-chain fatty acids regulate systemic bone mass and protect from pathological bone loss. Nature Communications, 9, 55.
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2

Optimized Micro-CT Imaging of Calcified Tissue

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µCT imaging was performed using the cone-beam Desktop Micro Computer Tomograph µCT 40 by SCANCO Medical AG, Bruettisellen, Switzerland. The settings were optimized for calcified tissue visualization at 55 kVp with a current of 145 µA and 200 ms integration time for 500 projections/180°. For the segmentation of 3D-Volumes an isotropic voxel size of 8.4 µm and an evaluation script with adjusted greyscale thresholds of the operating system Open VMS by SCANCO Medical was used.
Azizov V., Dietel K., Steffen F., Dürholz K., Meidenbauer J., Lucas S., Frech M., Omata Y., Tajik N., Knipfer L., Kolenbrander A., Seubert S., Lapuente D., Sokolova M.V., Hofmann J., Tenbusch M., Ramming A., Steffen U., Nimmerjahn F., Linker R., Wirtz S., Herrmann M., Temchura V., Sarter K., Schett G, & Zaiss M.M. (2020). Ethanol consumption inhibits TFH cell responses and the development of autoimmune arthritis. Nature Communications, 11, 1998.
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3

Quantitative Bone Analysis via μCT

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Bones were fixed in 4% paraformaldehyde overnight prior to the analyses. All µCT imaging was performed using the cone-beam Desktop Micro Computer Tomograph “µCT 40” by SCANCO Medical AG, Bruettisellen, Switzerland (http://www.scanco.ch/en/systems-solutions/specimen/microct40.html). The settings were optimized for calcified tissue visualization at 55 kVp with a current of 145 µA and 200 ms integration time for 500 projections/180°. For the segmentation of 3D-Volumes an isotropic voxel size of 8,4 µm and an evaluation script with adjusted greyscale thresholds of the operating system “Open VMS” by SCANCO Medical was used. For 3D-μCT analysis, measurement was conducted 1680 µm ca. 400 µm below the approximate middle of growth plate, left or right tibia metaphysis.
Taubmann J., Krishnacoumar B., Böhm C., Faas M., Müller D.I., Adam S., Stoll C., Böttcher M., Mougiakakos D., Sonnewald U., Hofmann J., Schett G., Krönke G, & Scholtysek C. (2020). Metabolic reprogramming of osteoclasts represents a therapeutic target during the treatment of osteoporosis. Scientific Reports, 10, 21020.
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4

Optimizing Bone Structure Analysis Using µCT

Check if the same lab product or an alternative is used in the 5 most similar protocols
µCT imaging was performed using the cone-beam Desktop Micro Computer Tomograph “µCT 40” by SCANCO Medical AG, Bruettisellen, Switzerland. The settings were optimized for calcified tissue visualization at 55 kVp with a current of 145 µA and 200 ms integration time for 500 projections/180°. For the segmentation of 3D-volumes, an isotropic voxel size of 8.4 µm and an evaluation script with adjusted grayscale thresholds of the operating system “Open VMS” by SCANCO Medical was used. Volume of interest tibia: The analysis of the bone structure was performed in the proximal metaphysis of the tibia, starting 0.43 mm from an anatomic landmark in the growth plate and extending 1.720 mm (200 tomograms) distally.
Tajik N., Frech M., Schulz O., Schälter F., Lucas S., Azizov V., Dürholz K., Steffen F., Omata Y., Rings A., Bertog M., Rizzo A., Iljazovic A., Basic M., Kleyer A., Culemann S., Krönke G., Luo Y., Überla K., Gaipl U.S., Frey B., Strowig T., Sarter K., Bischoff S.C., Wirtz S., Cañete J.D., Ciccia F., Schett G, & Zaiss M.M. (2020). Targeting zonulin and intestinal epithelial barrier function to prevent onset of arthritis. Nature Communications, 11, 1995.
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