Lead chromate

Sodium chromate, lead chromate, colcemid, and potassium chloride were purchased from Sigma-Aldrich (St. Louis, MO). Gurr’s buffer, trypsin/EDTA, sodium pyruvate, penicillin/streptomycin, and L-Glutamine were purchased from Invitrogen (Carlsbad, CA). Giemsa stain was purchased from Biomedical Specialties (Santa Monica, CA). Sodium dodecyl sulfate (SDS) was purchased from American Bioanalytical (Natick, MA). Crystal violet, methanol and acetone were purchased from J.T. Baker (Phillipsburg, NJ). Dulbecco’s minimal essential medium and Ham’s F-12 (DMEM/F-12) were purchased from Mediatech (Herndon, VA). Cosmic calf serum (CCS) was purchased from Hyclone (Logan, UT). Tissue culture dishes, flasks, and plasticware were purchased from Corning (Acton, MA)

RPMI was purchased from Mediatech (Manassas, VA). Penicillin/streptomycin, Gurr’s buffer, and trypsin/EDTA were purchased from Invitrogen Corporation (Grand Island, NY). Crystal violet, acetic acid, and methanol were purchased from J.T. Baker (Phillipsburg, NJ) and Fetal Bovine Serum (FBS) was purchased from Gibco Life Technologies (Grand Island, NY). Tissue culture dishes, flasks, and plasticware were purchased from BD (Franklin Lakes, NJ). Lead chromate, sodium chromate, potassium chloride (KCl) and demecolchicine were purchased from Sigma/Aldrich. Giemsa stain was purchased from Biomedical Specialties Inc. (Santa Monica, CA).

All plasticware was purchased from BD Falcon. Dulbecco’s Phosphate-Buffered Saline (DPBS), RPMI media with Glutagro was purchased from Corning. Potassium chloride, demecolcine, lead chromate, and sodium chromate were purchased from Sigma/Aldrich. Crystal violet, methanol and acetic acid were purchased from JT Baker. Microscope slides were purchased from Thermo Scientific. Giemsa stain was manufactured by Rica Chemical Co. Fetal Bovine Serum (FBS), Gurr’s buffer, trypsin, penicillin-streptomycin and sodium pyruvate was purchased from GIBCO Invitrogen Corporation.