Cell id kit

SERPINE1 expression and proliferation, migration and apoptosis assays were performed using six human head and neck squamous cell carcinoma cell lines (UM-SCC-22A, UM-SCC-22B, UM-SCC-74B, SCC9, SCC25 and FaDu). 293T cells were only used to generate lentivirus-containing supernatants.
UM-SCC-22A, UM-SCC-22B, UM-SCC-74B [58 (link)] and 293T (ATCC^® CRL-3216™; ATCC;http://www.lgcstandards-atcc.org) cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 100 U/mL streptomycin / penicillin and 2 mM glutamine (Life Technologies Ltd, UK). SCC-9 (ATCC^® CRL-1629™) and SCC-25 (ATCC^® CRL-1628™) HNSCC cell lines were grown in DMEM/F12 (1:1) containing 10% FBS, 100 U/mL streptomycin/penicillin, 2 mM glutamine and 0.4 μg/mL of hydrocortisone. FaDu (ATCC^® HTB-43™) HNSCC cell line from ATCC was grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 100 U/mL streptomycin/penicillin and 2 mM glutamine. All cell lines were cultured in a humidified atmosphere at 37°C and 5% of CO₂. Cell lines were authenticated comparing the STR profiles obtained using the Cell ID kit (Promega Corporation, Madison, WI) with the original STR profiles previously described (Supplementary files, table S2) [58 (link), 59 (link)].

Cell lines (of the serous subgroup) used for the assays were JC and JC- pl [13 (link)], OVCAR-3 and SK-OV-3 (both from the American Type Culture Collection (ATCC)). Normal human skin fibroblasts were provided by the department of Dermatology, LUMC, The Netherlands. The cell lines were grown in RPMI-1640, supplemented with 2 mM L-Glutamine (Gibco, Invitrogen, UK), 10% heat inactivated fetal calf serum (FCS) (Sigma), penicillin (50 units/mL) and streptomycin (50 μg/mL) (Invitrogen, UK). Normal skin fibroblasts were grown in Dulbecco’s modified Eagle medium (DMEM) (Invitrogen, UK), also supplemented with L-Glutamine and 10% FCS. The cultures were maintained in an incubator with humidified atmosphere at 37° C with 9% CO₂. The four human ovarian cancer cell lines were tested for their identity profile (ID) using a Cell ID™ kit from Promega. SK-OV-3 and OVCAR-3 were compared to their known profile of the ATCC and JC and JC-pl were cross referenced.