Quantum studio 6 flex

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Quantum Studio 6 flex is a real-time PCR system designed for gene expression and genotyping analysis. It features a 6-channel optical system and supports multiple sample formats, including 96-well plates, 8-tube strips, and individual tubes. The system provides precise temperature control and uniform thermal cycling for reliable and reproducible results.

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2 protocols using quantum studio 6 flex

Validating DEGs Expression by qRT-PCR

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We used quantitative reverse transcription (qRT)-PCR to verify the expression level of DEGs identified by RNA-Seq. The primers used are shown in Table 1 (39 (link), 44 (link)–50 (link)). Channel catfish EF-1 α was used as the internal parameter to homogenize the expression levels. The qRT-PCR was performed using quantum Studio 6 flex (life technologies, USA), and melting curves were generated at the end of the run to confirm the specificity. The 2^−ΔΔCt method was used to calculate the relative levels of DEGs (51 (link)).

Yang Y., Zhu X., Huang Y., Zhang H., Liu Y., Xu N., Fu G, & Ai X. (2022). RNA-Seq and 16S rRNA Analysis Revealed the Effect of Deltamethrin on Channel Catfish in the Early Stage of Acute Exposure. Frontiers in Immunology, 13, 916100.

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Validating RNA-seq Findings using qRT-PCR

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qRT-PCR was used to confirm the expression levels of DEGs identified by RNA-seq analysis. Primers used are listed in Table 1 (43 (link)–48 (link)). In channel catfish, EF-1 α was used as an endogenous control whereas gyrB was used for Y. ruckeri. qRT-PCR was performed using quantum Studio 6 flex (life technologies, USA) and melting curves were generated at the end of the run to confirm specificity. The 2^−ΔΔCt method was used to calculate the relative levels of DEGs (49 (link)).

Yang Y., Zhu X., Zhang H., Chen Y., Song Y, & Ai X. (2021). Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions. Frontiers in Immunology, 12, 775708.

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