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Elastin assay kit

Manufactured by Biocolor
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Sourced in China, United States, Ireland

The Elastin Assay Kit is a laboratory tool used to measure the amount of elastin in biological samples. It provides a quantitative assessment of elastin content through a colorimetric reaction. The kit includes necessary reagents and instructions to perform the analysis.

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Lab products found in correlation

Ccd 966sk, by American Type Culture Collection (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Ncounter platform, by NanoString (1 mentions) Mitochondrial membrane potential detection kit, by BD (1 mentions) Collagen assay kit, by Merck Group (1 mentions) Papain type 3, by Worthington (1 mentions) Gag assay kit, by Biocolor (1 mentions)

Spelling variants of this product

Fastin elastin assay kit (39 citations)

3 protocols using elastin assay kit

1

Bioactive Collagen Drink Impacts Skin Fibroblasts

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Elastin Collagen Peptide Complex Juice Drink (YAMII, Zhejiang Kazman Biotechnology Co., China; ingredients: water, fish collagen peptide powder (12%), banana powder, apple essence, cherry essence, γ-aminobutyric acid, grape powder, alma powder, acai powder, perilla seed powder, elderberry powder, brown rice powder, honey, vitamin C, erythritol, citric acid, fructose syrup, sucrose, flavor), human skin fibroblast (CCD-966Sk; ATCC®, CRL-1881), minimum essential medium with 10% FBS, 1% penicillin/streptomycin, and 1 mM sodium pyruvate (Gibco), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Amresco), phosphate-buffered saline (PBS, Gibco), DMSO (ECHO), collagen assay kit (Sircol), mitochondrial membrane potential detection kit (BD), 2′,7′-dichlorofluorescin diacetate (DCFH-DA, Sigma-Aldrich), elastin assay kit (Biocolor), RNA extraction kit (Genaid Biotech), nCounter® platform (NanoString Technologies), flow cytometry (BD) ELISA reader (BioTek).
Lin P., Hua N., Hsu Y.C., Kan K.W., Chen J.H., Lin Y.H., Lin Y.H, & Kuan C.M. (2020). Oral Collagen Drink for Antiaging: Antioxidation, Facilitation of the Increase of Collagen Synthesis, and Improvement of Protein Folding and DNA Repair in Human Skin Fibroblasts. Oxidative Medicine and Cellular Longevity, 2020, 8031795.
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2

Quantification of ECM Components in Tissue Constructs

Cited 1 time
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For collagen quantification, the lyophilized tissue construct samples (n=5 for each) were hydrolyzed in 12N HCL at 120°C for 3 hr. The hydrolyzed solution was then treated with a collagen assay kit (Sigma Aldrich, USA) following the manufacturer’s protocol to find the collagen amount in the samples (Jana and Lerman, 2019 (link), Jana, Lerman and Simari, 2015 (link)).
For glycosaminoglycan (GAG) quantification, the lyophilized tissue construct samples (n=5 for each) were digested in papain type III (Worthington Biochemical, USA) solution. The digested solution was then treated with a GAG assay kit (Blyscan, Biocolor, USA) following the manufacturer’s protocol to find the GAG amount in the samples (Jana and Lerman, 2019 (link), Jana, Lerman and Simari, 2015 (link)).
For elastin quantification, the lyophilized tissue construct samples (n=5 for each) were digested in 0.25M oxalic acid at 100°C for 1 hr. The digested solution was then treated with an elastin assay kit (Blyscan, Biocolor, USA) following the manufacturer’s protocol to find the elastin amount in the samples (Jana and Lerman, 2019 (link), Jana, Lerman and Simari, 2015 (link)).
Jana S, & Lerman A. (2020). Trilayered tissue construct mimicking the orientations of three layers of a native heart valve leaflet. Cell and tissue research, 382(2), 321-335.
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3

Quantifying Scaffold Elastin Release

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An elastin assay can be performed by using an Elastin Assay Kit (FastinTM, Biocolor Ltd., Belfast, Northern Ireland, UK) in which the amount of elastin being released from the scaffold can be measured. The scaffold was first degraded by soaking the scaffold in DPBS at 37 °C at different time points. The DPBS containing degraded contents of the scaffold was then collected and underwent the elastin assay by using the FastinTM Elastin Assay Kit. The kit contains a few reagents and steps which involve elastin isolation, recovery of the elastin–dye complex, release of the elastin bound dye and, lastly, the elastin measurement, which is at 513 nm.
Zulkiflee I., Amirrah I.N., Fadilah N.I., Wee M.F., Yusop S.M., Maarof M, & Fauzi M.B. (2023). Characterization of Dual-Layer Hybrid Biomatrix for Future Use in Cutaneous Wound Healing. Materials, 16(3), 1162.
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