Gsk2606414 hy 18072

Antibodies against GRP78 (ab21685), ATF6 (ab122897), p-PERK (ab192591) and p-eIF2α (Ser51) (ab32157) were obtained from Abcam (Cambridge, UK). Antibodies against total PERK (sc-13073) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against p-IRE1 (S724) (CY5605), eIF2α (AB3335), and β-actin (AB0061) were purchased from Abways Technology (Shanghai, China). Antibodies against total IRE1 (3294S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Thapsigargin (Tg) (Abcam, ab120286), GSK2606414 (HY-18072) (MedChem Express, Monmouth Junction, NJ, USA), APY29 (HY-17537) (MedChem Express, Monmouth Junction, NJ, USA) and 4μ8C (HY-19707) (MedChem Express, Monmouth Junction, NJ, USA) were dissolved in DMSO.

Human chordoma cell line CM319^{2 (link),58 (link)} was a gift from Dr. Wen Yan-hua (Department of Orthopedic Oncology, Tang Du Hospital, Xi’an, China) and was maintained at 37 °C under 5% CO₂ in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 units/ml), and streptomycin (100 μg/ml). Human chordoma cell line UCH1^{13 (link),39 (link),40 (link)} was obtained from American Type Culture Collection (#CRL­3217) and was maintained in rat tail type I collagen (BD Biosciences)-coated flasks at 37 °C under 5% CO₂ in Iscove/RPMI (4:1) medium (Gibco, USA), supplemented with 10% FBS (Gibco, USA), penicillin (100 units/ml), streptomycin (100 μg/), and 1% (v/v) l-glutamine (Gibco, USA). Doxo (#HY-15142A), Irino (#HY-16562), and GSK2606414 (#HY-18072) were purchased from MedChemExpress (Shanghai, China). The final concentration of dimethylsulfoxide is <0.05% (v/v). No mycoplasma was detected in the CM319 and UCH1 cell lines.