Anti-EGR1 (22008-1-AP) rabbit polyclonal antibody (pAb), anti-Lamin B (66095-1-Ig), and anti-tubulin (66031-1-Ig) were purchased from ProteinTech (Rosemont, IL, USA). Antibodies against IκBα (4814S), p-IκBα (Ser32) (2859S), and p-p65 (8242S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-p65 rabbit pAb (ab7970) and anti-Tax mouse monoclonal antibody (mAb, sc-57872) were bought from Abcam (Cambridge, MA, USA) and Santa Cruz Biotechnology (Santa Cruz, CA, USA), respectively. The anti-β-actin mouse mAb (60008-1-Ig), as well as the following immunoglobulin G (IgG) reagents: horseradish peroxidase-linked goat anti-mouse (00001-1) and goat anti-rabbit (00001-2), were purchased from ProteinTech. The NF-κB inhibitor, Bay 11-7082 (B5556), and phytohemagglutinin (PHA; L8902) were purchased from Sigma (St Louis, MO, USA). 5-(and-6)-Carboxy fluorescein diacetate, succinimidyl ester (CFSE) (C1157), EGR1 siRNA (1299001), and Lipofectamine 2000 (11668-019) were purchased from Invitrogen (Carlsbad, CA, USA). Cycloheximide (CHX; C8030) was purchased from Solarbio (Beijing, China).
Anti tubulin 66031 1 ig
Manufactured by Proteintech
Sourced in United States, China
Anti-tubulin (66031-1-Ig) is a primary antibody that specifically binds to the tubulin protein. Tubulin is a core component of microtubules, which are essential cytoskeletal structures responsible for various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000 11668 019, by Thermo Fisher Scientific (1 mentions)
Bay 11 7082 b5556, by Merck Group (1 mentions)
Horseradish peroxidase conjugated anti rabbit or mouse igg h l, by Proteintech (1 mentions)
Anti cyclin e1 11554 1 ap, by Proteintech (1 mentions)
Tissuelyser 2, by Qiagen (1 mentions)
Chemidoc touch imaging system, by Bio-Rad (1 mentions)
Mouse monoclonal antibody to ha 901503, by BioLegend (1 mentions)
Anti rabbit secondary antibody a4914, by Merck Group (1 mentions)
Rabbit anti ha 3724, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca kit, by Solarbio (1 mentions)
Ipfl00010, by Merck Group (1 mentions)
Anti β actin 66009 1 ig, by Proteintech (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
4 protocols using anti tubulin 66031 1 ig
1
Antibody Panel for NF-κB Signaling
2
Liver Carcinoma Protein Expression Analysis
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Twelve human liver carcinomas and adjacent normal tissues were obtained from the Tumor Tissue Bank of the National Translational Science Centre for Molecular Medicine. The tissues were obtained with informed consent, but clinical information was not available over time. Tissue samples were pretreated with TissueLyser II (Qiagen, Shanghai, China), and the supernatants were collected by centrifugation. Western blotting was performed with the corresponding primary antibodies (anti-LCK antibody, 2417S, CST; anti-CDK2 antibody, 10122-1-AP, Proteintech; anti-CDK4 antibody, 11026-1-AP, Proteintech; anti-cyclin E1, 11554-1-AP, Proteintech; anti-cyclin D1, 26939-1-AP, Proteintech; anti-tubulin, 66031-1-Ig, Proteintech, Wuhan, China). Horseradish peroxidase-conjugated anti-rabbit or mouse IgG (H + L) (Proteintech) was used to detect the primary antibodies and incubated at room temperature for 1 h. All antibodies were diluted using antibody dilution buffer (WB100D, NCM Biotech, Suzhou, China). Western blotting images were captured using a ChemiDocTMTouch Imaging System (Bio-Rad, Hercules, CA, USA).
3
Antibody Validation for Western Blotting
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All primary antibodies were used at a dilution of 1:1000–3000 in TBST buffer with 5% non-fat milk for Western blotting. Anti-PRMT5 (79998), anti-TRAF6 (8028S), anti-MEP50 (2018S), rabbit anti-Myc-tag (2278), and rabbit anti-HA (3724) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibody to HA (901503) was purchased from Biolegend (San Diego, CA, USA). Rabbit anti-Flag (F7425), mouse anti-Flag (F3165), peroxidase-conjugated anti-mouse secondary antibody (A4416), and anti-rabbit secondary antibody (A4914) were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-H4R3me2s (A-3718-100) was purchased from EpigenTek (Farmingdale, NY, USA). Anti-Tubulin (66031-1-Ig) and anti-H4 (16047-1-AP) were purchased from Proteintech (Rosemont, IL, USA).
4
Western Blotting Assay for Autophagy and Apoptosis Markers
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Western blot experiments were performed as previously described [51 (link)]. In brief, whole cell or tissue extracts were prepared with RIPA lysis buffer (Beyotime, P0013B) supplemented with phosphatase inhibitors and protease inhibitors. After lysate incubation for 30 min on ice, supernatant protein was quantified using a BCA kit (Solarbio, PC0020). Equal amounts of protein were subjected to SDS-PAGE and then transferred to nitrocellulose membranes (Millipore, IPFL00010). Membranes were blocked at room temperature for 2 h in 5% milk in TBST, followed by incubation with primary antibodies in 4 °C overnight. Signals were visualized by the imaging system after incubation with secondary antibody.
Antibodies to SQSTM1/P62(#5114), LC3A/B (#12741), Beclin-1(#3495), Phospho-ULK1(Ser757) (#6888), ULK1 (#8054), Phospho-mTOR (Ser2448) (#5536), mTOR (#2983), Bax(#14796), BCL2(#3498), Cleaved Caspase-3(#9664), GAPDH(#2118) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin (#66009-1-Ig) and anti-tubulin (#66031-1-Ig) were purchased from ProteinTech (Wuhan, China). Anti-KLF6 (#sc-365633) was obtained from SantaCruz (Dallas, TX, USA).
Antibodies to SQSTM1/P62(#5114), LC3A/B (#12741), Beclin-1(#3495), Phospho-ULK1(Ser757) (#6888), ULK1 (#8054), Phospho-mTOR (Ser2448) (#5536), mTOR (#2983), Bax(#14796), BCL2(#3498), Cleaved Caspase-3(#9664), GAPDH(#2118) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin (#66009-1-Ig) and anti-tubulin (#66031-1-Ig) were purchased from ProteinTech (Wuhan, China). Anti-KLF6 (#sc-365633) was obtained from SantaCruz (Dallas, TX, USA).
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