Completeultra mini tablets

Manufactured by Roche

Sourced in Switzerland, United States

The COmpleteUltra Mini tablets are a product offered by Roche for laboratory equipment. The tablets serve as a core function, but a detailed unbiased description cannot be provided while maintaining conciseness.

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Lab products found in correlation

Hiseq platform, by Illumina (1 mentions) Miseq platform, by Illumina (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Tbs blocking buffer, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Anti h3cit, by Abcam (1 mentions)

2 protocols using completeultra mini tablets

Transcriptomic and Proteomic Analysis of Xanthosoma tulumensis

Cited 1 time

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Specimens of X. tulumensis were collected in 2012 and 2014 for transcriptomic and proteomic analyses in cave diving expeditions to the Yucatan, Mexico, see also [12 (link)]. Several complete individuals were dissected in TBE buffer and preserved in RNAlater to sequence a body transcriptome (BodyT) on the Illumina HiSeq platform (San Diego, USA). The venom delivery systems of 25 specimens (including venom gland, venom duct and venom reservoir) were dissected and preserved in RNAlater to generate the venom gland transcriptome (VgT) on the MiSeq Illumina platform (San Diego, CA, USA). For proteomic work venom delivery systems of 25 specimens were dissected in sterile PBS buffer and preserved in a protease inhibitor solution using cOmpleteUltra Mini tablets (Roche, Basel, Switzerland) dissolved in PBS and RNAse free HCP water following the manufacturer’s protocol, see Figure 9 for complete work flow.

von Reumont B.M., Undheim E.A., Jauss R.T, & Jenner R.A. (2017). Venomics of Remipede Crustaceans Reveals Novel Peptide Diversity and Illuminates the Venom’s Biological Role. Toxins, 9(8), 234.

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Western Blot Analysis of Protein Expression

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Protein was isolated from IVC segments or thrombus using RIPA buffer (ThermoScientific, Rockford, Ill., USA) with dissolved cOmplete ULTRA mini tablets (Roche, Mannheim, Germany). Proteins were electrophoretically separated on NuPAGE 4–12% Bis Tris gels (Invitrogen, Carlsbad, Calif., USA) and blotted onto PVDF membranes (Millipore, Billerica, Mass., USA). Nonspecific binding was blocked with starting block (TBS) blocking buffer (ThermoScientific). Antibodies used included: anti-H₃-Cit (1/500 dil., Abcam, Cambridge, Mass., USA), anti-cathepsin G (1/20,000 dil., Novus, Littleton, Colo., USA), anti-TFPI (2 μg/ml dil., Novus), anti-GAPDH (1/1,000 dil., Santa Cruz, Dallas, Tex., USA), anti-fibrin (clone 59D8, 1/1,000 dil., a gift from Dr. Charles Esmon, Oklahoma Medical Research Foundation, Oklahoma City) [25 (link)]. Immunoreactive bands were visualized with SuperSignal West Pico.
Chemiluminescent Substrate (ThermoScientific) and densitometry was performed using Image J software. Optical densities were summed and normalized to GAPDH.

Obi A.T., Andraska E., Kanthi Y., Luke C.E., Elfline M., Madathilparambil S., Siahaan T.J., Jaffer F.A., Wakefield T.W., Raghavendran K, & Henke P.K. (2016). Gram-Negative Pneumonia Alters Large-Vein Cell-Adhesion Molecule Profile and Potentiates Experimental Stasis Venous Thrombosis. Journal of vascular research, 53(3-4), 186-195.

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