Anti granzymeb pe cyanine7

Mouse or human cells were stained with the following antibodies at a dilution ratio of 1:50: anti-CD45 PerCP-Cy5.5 (BD biosciences, 550994), anti-CD11b FITC (Biolegend, 101206), anti-CD86 APC (Biolegend, 105011), anti-CD206 PE (Biolegend, 141706), anti-CD8a FITC (Biolegend, 100706), anti-CD4 APC (Biolegend, 100516), anti-MHCI PE (Biolegend, 114607), anti-Foxp3 PE (BD biosciences, 563101), anti-IFN-γ PE (BD biosciences, 554412), anti-TNF-α PE/Cyanine7 (Biolegend, 506324), anti-Granzyme B PE/Cyanine7 (Biolegend, 396410), anti-PD1 PE/Cyanine7 (Biolegend, 109110), anti-Tim3 PE (Biolegend, 134003), anti-CTLA4 PE (Biolegend, 106305) and anti-LAG3 PE (Biolegend, 125207). For intracellular staining, the cells were fixed in Fixation and Permeabilization kit (eBioscience, 00-5521-00). After washed with Fixation and Permeabilization buffer, the cells were stained intracellularly for 30 min in the dark. All samples were acquired on BD LSRFortessa (BD Biosciences) or ACEA NovoCyte (ACEA Biosciences), and were analyzed using FlowJo (FlowJo LLC) or NovoExpress software (ACEA Biosciences).