Elx50 automatic microplate strip washer

The SpectraMax® M5 microplate reader, manufactured by Molecular Devices, LLC in San Jose, California, USA, offers multiple detection modes including absorbance, fluorescence, fluorescence polarization, time-resolved fluorescence, and chemiluminescence. It has a wide light reading range of 200 to 1000 nm with 1 nm increments. The reader can accommodate various microplate formats, from standard 6-well to 384-well, and its 4-zone system allows for precise temperature control up to 50 °C, ensuring excellent stability for temperature-sensitive assays. Additionally, the instrument features an internal shaker with three different speed settings (low, medium, and high) for efficient solution mixing. The reader is controlled and operated using the SoftMax^® Pro Enterprise software, which is included with the device. Other laboratory equipment used in the study includes the ELx50 automatic microplate strip washer from Bio-Tek Instruments Inc. in Winooski, USA, the Milli-Q water purification system from Labo, Millipore Ltd in Bedford, USA, the microprocessor laboratory pH meter from Mettler-Toledo International Inc. in Zürich, Switzerland, the Clifton Cyclone CM1 vortex mixer from Weston, England, and the Sanyo MDF-U5312 biomedical freezer from Onoda, Japan.

The SpectraMax^® M5 microplate reader Molecular Devices, LLC, (San Jose, California, USA) was used. It has multi-detection modes (absorbance, fluorescence, fluorescence polarization, time-resolved fluorescence, and chemiluminescence). The reader can read lights in the range of 200–1000 nm with 1 nm increments. The reader can adapt microplates ranging from standard 6-well to 384-well, and its 4-zone system can control temperatures up to 50 °C, allowing temperature-sensitive assays to be performed with excellent stability. The instrument also featured an internal shaker with three different speeds (low, medium, and high) for mixing solutions. The reader is controlled and operated by the SoftMax^® Pro Enterprise software, provided with the reader. ELx50 automatic microplate strip washer was a product of Bio-Tek Instruments Inc. (Winooski, USA). An incubator (model MINI/18) was obtained from Genlab Ltd. (Widnes, UK). Milli-Q water purification system (Labo, Millipore Ltd., Bedford, USA). Microprocessor laboratory pH meter (Mettler-Toledo International Inc., Zürich, Switzerland). Vortex (Clifton cyclone CM1: Weston, England). Biomedical freezer (Sanyo MDF-U5312: Onoda, Japan).