P. falciparum 3D7 parasites were cultured as previously described (101 (link)). In short, parasites were cultured in human AB+ erythrocytes (Interstate Blood Bank, Memphis, TN, USA) at 3%–10% parasitemia in a complete culture medium (5% hematocrit). Complete culture medium consisted of RPMI 1,640 medium (Gibco #32404014) supplemented with gentamicin (45 µg/mL final concentration; Gibco #15710064), HEPES (40 mM; Fisher #BP3101), NaHCO3 (1.9 mg/mL; Sigma #SX03201), NaOH (2.7 mM; Fisher #SS266-1), hypoxanthine (17 µg/mL; Alfa Aesar #A11481-06), L-glutamine (2.1 mM; Corning #25005 Cl), D-glucose (2.1 mg/mL; Fisher #D16-1), and 10% (vol/vol) human AB+ serum (Valley Biomedical #HP1022). Parasites were cultured at 37°C in an atmosphere of 5% O2, 5% CO2, and 90% N2.
Ss266 1
Manufactured by Thermo Fisher Scientific
The SS266-1 is a stainless steel floor stand designed for laboratory use. It provides a sturdy and adjustable platform to support various types of laboratory equipment. The product dimensions and specifications are available upon request.
Automatically generated - may contain errors
2 protocols using ss266 1
1
Plasmodium falciparum 3D7 Culture Protocol
2
Culturing P. falciparum 3D7 Parasites
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P. falciparum 3D7 parasites were cultured as previously described (101 (link)). In short, parasites were cultured in human AB+ erythrocytes (Interstate Blood Bank, Memphis, TN, USA) at 3 – 10% parasitemia in complete culture medium (5% hematocrit). Complete culture medium consisted of RPMI 1640 medium (Gibco #32404014) supplemented with gentamicin (45 µg/ml final concentration; Gibco #15710064), HEPES (40 mM; Fisher #BP3101), NaHCO3 (1.9 mg/ml; Sigma #SX03201), NaOH (2.7 mM; Fisher #SS266-1), hypoxanthine (17 µg/ml; Alfa Aesar #A11481-;06), L-glutamine (2.1 mM; Corning #25005Cl), D-glucose (2.1 mg/ml; Fisher #D16-1), and 10% (vol/vol) human AB+ serum (Valley Biomedical #HP1022). Parasites were cultured at 37°C in an atmosphere of 5% O2, 5% CO2, and 90% N2.
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