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Jetquick blood cell culture dna spin kit

Manufactured by Genomed
Sourced in Germany

The JETQUICK Blood & Cell Culture DNA Spin Kit is a laboratory product designed for the rapid extraction and purification of genomic DNA from various sample types, including whole blood, buffy coat, and cultured cells. The kit utilizes a spin column-based method to efficiently capture and purify DNA, making it suitable for downstream applications such as PCR, sequencing, and other molecular biology techniques.

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2 protocols using jetquick blood cell culture dna spin kit

1

DNA Extraction and Genotyping from Whole Blood

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Genomic DNA was purified from whole blood using JETQUICK Blood & Cell Culture DNA Spin Kit (Genomed GmbH, Löhne, Germany) according to the manufacturer's protocol. Resulting DNA samples were aliquoted and stored at −80°C. For further analysis, DNA was normalized to 50 ng/μl with an automated robotic platform (Microlab Star, Hamilton, Bonaduz, Switzerland). For quality control, each sample was analyzed with a 0.8% agarose gel. Genotyping was performed using SimpleProbes (TIB Molbiol, Berlin, Germany) on LightCycler 480 (Roche Diagnostics, Basel, Switzerland), according to the manufacturer's instructions.
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2

Saliva DNA Extraction and FUT2 Genotyping

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Host DNA was extracted from saliva samples using the JETQUICK Blood & cell culture DNA spin kit (Genomed) following the manufacturer instructions. The secretor status was investigated by genotyping the FUT2 gene by PCR-RFLP as described previously25 (link).
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