Anti cd8a percp cy5.5 hit8a

Manufactured by BioLegend

Sourced in United States

Anti-CD8a PerCP/Cy5.5 (HIT8a) is a fluorescently-labeled monoclonal antibody that binds to the CD8a protein, which is expressed on the surface of certain T cells. This product is intended for use in flow cytometry applications to identify and characterize CD8a-positive cells.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd197 ccr7 pe, by BioLegend (1 mentions) Anti cd3 fitc clone ucht1, by BD (1 mentions) Anti cd4 v500 rpa t4, by BD (1 mentions) Facsaria 3, by BD (1 mentions)

Close spelling variants of this product

PerCP-Cy5.5–anti-CD8a PerCP/Cy5.5-CD8a Anti-CD8a-PerCP/Cy5.5 CD8a-PerCp-Cy5.5 CD8a-PerCP PerCP-Cy5.5 HIT8a Anti-CD8a

2 protocols using anti cd8a percp cy5.5 hit8a

Multiparameter Immunophenotyping of T-cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescein-conjugated anti-human mAbs, including anti-CD3 FITC (clone: UCHT1), anti-CD4 V500 (RPA-T4), anti-CD8 APC/Cy7 (SK1), anti-CD56 PE (B159), and anti-CD279 (PD-1) PE (EH12.1, also known as EH12) were purchased from BD Pharmingen. Meanwhile, the other anti-human mAbs including anti-CD3 APC (HIT3a), anti-CD8a PerCP/Cy5.5 (HIT8a), anti-CD38 FITC (HIT2), anti-CD63 FITC (H5C6), anti-CD69 APC (FN50), anti-CD122 (IL-2Rβ) APC (TU27), anti-CD183 (CXCR3) PE/Cy7 (G025H7), anti-CD183 (CXCR3) APC (G025H7), anti-CD192 (CCR2) APC/Cy7 (K036C2), anti-CD196 (CCR6) PE (G034E3), anti-CD197 (CCR7) PE (G043H7), anti-CD276 (B7-H3) PE (DCN.70), anti-CD45RO FITC (UCHL1), anti-CD197 (CCR7) PE/Cy7 (G043H7), anti-IFN-γ APC/Cy7 (4S.B3), anti-IL-4 PE/Cy7 (MP4-25D2), anti-IL-17A FITC (BL168), and anti-FOXP3 PE (206D) were purchased from BioLegend.

Zeng W., Liu Z., Liu X., Zhang S., Khanniche A., Zheng Y., Ma X., Yu T., Tian F., Liu X.R., Fan J, & Lin Y. (2017). Distinct Transcriptional and Alternative Splicing Signatures of Decidual CD4+ T Cells in Early Human Pregnancy. Frontiers in Immunology, 8, 682.

+ Open protocol

+ Expand

Flow Cytometric Isolation of CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human DMCs and PBMCs were incubated with fluorescein-conjugated anti-human monoclonal antibodies (mAbs) including anti-CD3 FITC (clone: UCHT1; BD Biosciences, USA), anti-CD4 V500 (RPA-T4; BD Biosciences, USA), and anti-CD8a PerCP/Cy5.5 (HIT8a; BioLegend, USA) in 1 mL PBS containing 3% (v/v) fetal bovine serum (FBS) at 4°C for 30 min. Paired dCD8 and pCD8 T cells were isolated from the DMCs and PBMCs, respectively, by sorting on an FACSAria III (BD Biosciences, USA) based on the surface expression of CD3, CD4, and CD8 (CD3⁺CD8⁺CD4⁻) with a purity always greater than 95%.

Zeng W., Liu X., Liu Z., Zheng Y., Yu T., Fu S., Li X., Zhang J., Zhang S., Ma X., Liu X.R., Qin X., Khanniche A., Zhang Y., Tian F, & Lin Y. (2018). Deep Surveying of the Transcriptional and Alternative Splicing Signatures for Decidual CD8+ T Cells at the First Trimester of Human Healthy Pregnancy. Frontiers in Immunology, 9, 937.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!